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master's thesis
Određivanje haplotipova HLA-DRB analizom mikrosatelita D6S2878 u hrvatskoj populaciji
Zagreb: University of Zagreb, Faculty of Science, 2009. urn:nbn:hr:217:383030
Bašić, Marijana
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Bašić, M. (2009). Određivanje haplotipova HLA-DRB analizom mikrosatelita D6S2878 u hrvatskoj populaciji (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:383030

Bašić, Marijana. "Određivanje haplotipova HLA-DRB analizom mikrosatelita D6S2878 u hrvatskoj populaciji." Master's thesis, University of Zagreb, Faculty of Science, 2009. https://urn.nsk.hr/urn:nbn:hr:217:383030

Bašić, Marijana. "Određivanje haplotipova HLA-DRB analizom mikrosatelita D6S2878 u hrvatskoj populaciji." Master's thesis, University of Zagreb, Faculty of Science, 2009. https://urn.nsk.hr/urn:nbn:hr:217:383030

Bašić, M. (2009). 'Određivanje haplotipova HLA-DRB analizom mikrosatelita D6S2878 u hrvatskoj populaciji', Master's thesis, University of Zagreb, Faculty of Science, accessed 25 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:383030

Bašić M. Određivanje haplotipova HLA-DRB analizom mikrosatelita D6S2878 u hrvatskoj populaciji [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2009 [cited 2024 November 25] Available at: https://urn.nsk.hr/urn:nbn:hr:217:383030

M. Bašić, "Određivanje haplotipova HLA-DRB analizom mikrosatelita D6S2878 u hrvatskoj populaciji", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2009. Available at: https://urn.nsk.hr/urn:nbn:hr:217:383030

Metadata
TitleOdređivanje haplotipova HLA-DRB analizom mikrosatelita D6S2878 u hrvatskoj populaciji
Title (english)Definition of HLA-DRB haplotypes by microsatellite D6S2878 analysis in a Croatian population
AuthorMarijana Bašić
MentorZorana Grubić (mentor)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2009, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
U ovom radu analizirali smo alele mikrosatelita D6S2878, u svrhu određivanja haplotipova HLA-DRB, unutar skupine od 53 nesrodne zdrave osobe. Broj alela mikrosatelita D6S2878 u jednom haplotipu ovisi o tome koliki je broj gena DRB (aktivnih i pseudogena) u pojedinom haplotipu HLA. Analizom haplotipova HLA-DR1, DR10 te haplotipova DR52 uočili smo po dva alela mikrosatelita D6S2878. Kraći alel D6S2878 u haplotipu HLA-DR1 (alel D6S2878-136pb) odgovarao je alelu na pseudogenu DRB6, a duži alelima na lokusu DRB1 (alel D6S2878-154pb i alel D6S2878-161pb). U haplotipu HLA-DR52 alel D6S2878 koji je odgovarao alelima lokusa DRB1 (aleli D6S2878 dužine od 172pb do 206pb) bio je kraći od alela za lokus DRB3 (aleli D6S2878 dužine od 180pb do 214pb). Na haplotipovima HLA-DR51 i HLADR53 uočili smo po 3 alela mikrosatelita D6S2878. Kod haplotipova HLA-DR51 najkraći alel D6S2878 odgovarao je pseudogenu DRB6 (aleli D6S2878-144pb, 152pb, 154pb i 174pb) dok su duži aleli (od 176pb do 204pb) odgovarali alelima lokusa DRB1, odnosno aleli D6S2878 dužine od 180pb do 220pb alelima lokusa DRB5. Pseudogen DRB7 u haplotipovima HLA-DR53 imao je najkraće alele D6S2878 od 135pb, dok su dužine alela D6S2878 od 170pb do 194pb odgovarale alelima DRB1, a aleli D6S2878 od 176pb do 182pb alelima DRB4. Kod alela DRB1*08 uočen je samo jedan alel D6S2878 i to dužine od 170pb i 176pb.
Abstract (english)
In this study, we analyzed alleles at D6S2878 microsatellite among 53 unrelated subjects in order to define HLA-DRB haplotypes. Number of D6S2878 alleles detected in one haplotype depends on the number of DRB genes (active and pseudogenes) contained by a specific haplotype. Analysis of HLA-DR1, and HLA-DR52 haplotypes revealed two D6S2878 alleles. Shorter allele in HLA-DR1 haplotype (D6S2878-136bp) matched the allele on pseudogene DRB6, while longer matched the alleles on DRB1 locus (D6S2878- 154bp and D6S2878-161bp). In HLA-DR52 haplotype D6S2878 allele which matched alleles on DRB1 locus (172bp-206bp) was shorter than allele which matched DRB3 locus (180bp-214bp). We observed three D6S2878 microsatellite alleles HLA-DR51 and HLADR53 haplotypes. The shortest allele in HLA-DR51 haplotype matched the allele on pseudogene DRB6 (D6S2878-144bp, 152bp, 154bp and 174bp), while the longer alleles (176bp-204pb) corresponded to alleles at DRB1 locus or in the case of alleles of the lenght 180bp-220bp to the alleles at DRB5 locus. Pseudogene DRB7 in HLA-DR53 haplotypes had the shortest D6S2878 allele (D6S2878-135bp), while alleles D6S2878 of the lenght 170bp-194bp matched alleles at DRB1 locus and alleles D6S2878 of the lenght 176bp-182bp matched alleles at DRB4 locus. Allele DRB1*08 displayed only one D6S2878 allele which could be either 170bp or 176bp long.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:383030
Study programmeTitle: Integrated undergraduate and graduate university study of Biology and Chemistry Education; specializations in: Integrated undergraduate and graduate university study of Biology and Chemistry Education
Course: Integrated undergraduate and graduate university study of Biology and Chemistry Education
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: magistar/magistra edukacije biologije i kemije (magistar/magistra edukacije biologije i kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2018-03-06 09:42:52