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master's thesis
Određivanje genotoksičnog učinka talijeva(I) acetata u vodenoj leći Lemna minor L. komet-testom
Zagreb: University of Zagreb, Faculty of Science, 2009. urn:nbn:hr:217:140032
Brnjilović, Petar
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Brnjilović, P. (2009). Određivanje genotoksičnog učinka talijeva(I) acetata u vodenoj leći Lemna minor L. komet-testom (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:140032

Brnjilović, Petar. "Određivanje genotoksičnog učinka talijeva(I) acetata u vodenoj leći Lemna minor L. komet-testom." Master's thesis, University of Zagreb, Faculty of Science, 2009. https://urn.nsk.hr/urn:nbn:hr:217:140032

Brnjilović, Petar. "Određivanje genotoksičnog učinka talijeva(I) acetata u vodenoj leći Lemna minor L. komet-testom." Master's thesis, University of Zagreb, Faculty of Science, 2009. https://urn.nsk.hr/urn:nbn:hr:217:140032

Brnjilović, P. (2009). 'Određivanje genotoksičnog učinka talijeva(I) acetata u vodenoj leći Lemna minor L. komet-testom', Master's thesis, University of Zagreb, Faculty of Science, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:140032

Brnjilović P. Određivanje genotoksičnog učinka talijeva(I) acetata u vodenoj leći Lemna minor L. komet-testom [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2009 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:217:140032

P. Brnjilović, "Određivanje genotoksičnog učinka talijeva(I) acetata u vodenoj leći Lemna minor L. komet-testom", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2009. Available at: https://urn.nsk.hr/urn:nbn:hr:217:140032

Metadata
TitleOdređivanje genotoksičnog učinka talijeva(I) acetata u vodenoj leći Lemna minor L. komet-testom
Title (english)Determination of thallium(I) acetate genotoxicity in common duckweed Lemna minor L. by comet assay
AuthorPetar Brnjilović
MentorMirjana Pavlica (mentor)
Committee memberMirjana Pavlica (predsjednik povjerenstva)
Committee memberDavor Kovačević (član povjerenstva)
Committee memberDubravka Matković-Čalogović (član povjerenstva)
Committee memberInes Radanović (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2009, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Koncentracija teškog metala talija u okolišu kontinuirano raste, a kako ga biljke mogu apsorbirati tako talij putem hranidbenih lanaca postaje dostupan životinjama i čovjeku. Cilj ovog istraživanja bio je pomoću komet-testa kao nespecifičnog biomarkera genotoksičnosti odrediti genotoksičan učinak talijeva(I) acetata u vodenoj leći Lemna minor L. Jezgre stanica vodene leće su prvo u uvjetima in vitro tretirane vodikovim peroksidom kao modelnim genotoksikantom čime je potvrđena osjetljivost komet-testa za utvrđivanje direktne genotoksičnosti na vodenoj leći. Vodena leća je sedam dana izlagana u uvjetima in vivo različitim koncentracijama talijeva(I) acetata. Rezultati komet-testa pokazali su blagi genotoksični učinak dvaju najviših koncentracija talija. Kako bi utvrdili djeluje li talij direktno ili indirektno na molekulu DNA izveden je i acelularni komet-test u uvjetima in vitro na jezgrama vodene leće. Rezultati dobiveni celularnim i acelularnim komet-testom ukazuju na indirektno oštećenje DNA uzrokovano talijevim(I) acetatom.
Abstract (english)
Concentration of the heavy metal thallium in the environment has been continuously increasing. Since thallium can be absorbed by plants it becomes available for the higher organisms including men through food chains. The main goal of this work was to determine genotoxicity of thallium(I) acetate by comet assay as a non-specific genotoxicity biomarker in common duckweed Lemna minor L. Duckweed cell nuclei in in vitro conditions were treated with hydrogen peroxide as a model genotoxicant in order to verify the comet assay sensitivity. Results are showing direct genotoxic effect. Common duckweed plants were in vivo exposed to different thallium(I) acetate concentrations for seven days. The results of comet assay showed a mild genotoxicity of two highest concentrations of thallium. In order to determine whether thallium directly or indirectly affect DNA an acellular comet assay was performed in vitro on common duckweed nuclei. Results following both cellular and acellular comet assays implies indirect DNA damage caused by thallium(I) acetate.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:140032
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2018-03-07 09:21:27