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master's thesis
Preusmjeravanje adenovirusa tipa 5 na integrin α4β1
Zagreb: University of Zagreb, Faculty of Science, 2009. urn:nbn:hr:217:228201
Jelušić, Tihana
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Jelušić, T. (2009). Preusmjeravanje adenovirusa tipa 5 na integrin α4β1 (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:228201

Jelušić, Tihana. "Preusmjeravanje adenovirusa tipa 5 na integrin α4β1." Master's thesis, University of Zagreb, Faculty of Science, 2009. https://urn.nsk.hr/urn:nbn:hr:217:228201

Jelušić, Tihana. "Preusmjeravanje adenovirusa tipa 5 na integrin α4β1." Master's thesis, University of Zagreb, Faculty of Science, 2009. https://urn.nsk.hr/urn:nbn:hr:217:228201

Jelušić, T. (2009). 'Preusmjeravanje adenovirusa tipa 5 na integrin α4β1', Master's thesis, University of Zagreb, Faculty of Science, accessed 26 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:228201

Jelušić T. Preusmjeravanje adenovirusa tipa 5 na integrin α4β1 [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2009 [cited 2024 November 26] Available at: https://urn.nsk.hr/urn:nbn:hr:217:228201

T. Jelušić, "Preusmjeravanje adenovirusa tipa 5 na integrin α4β1", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2009. Available at: https://urn.nsk.hr/urn:nbn:hr:217:228201

Metadata
TitlePreusmjeravanje adenovirusa tipa 5 na integrin α4β1
Title (english)Retargeting adenovirus type 5 to integrin α4β1
AuthorTihana Jelušić
MentorAndreja Ambriović Ristov (mentor)
Committee memberAndreja Ambriović Ristov (predsjednik povjerenstva)
Committee memberDubravka Hranilović (član povjerenstva)
Committee memberMladen Krajačić (član povjerenstva)
Committee memberMaja Matulić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2009, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Gensko preusmjeravanje Adenovirusa tipa 5 (Ad5) ugradnjom sljedova u vlakno bi trebalo omogućiti ciljani unos gena. Cilj je ovog istraživanja konstruirati replikacijski defektan adenovirusni vektor uvođenjem ciljnog liganda CPLDIDFYC za integrin α4β1 u HI petlju glave vlakna, kako bi se preusmjerio Ad5 na integrin α4β1 (poznat i pod imenom VLA-4, engl. very late antigen). Rekombinantni Ad5VLA-4 virus konstruiran je manipulacijom cjelovitog Ad5 genoma kao plazmida u E. coli, pri čemu je korištena homologna rekombinacija u bakterijama. Ad5VLA-4 i kontrolni virus Ad5wt bez ciljnog slijeda, umnoženi su u kulturi bubrežnih stanica ljudskog embrija 293 i pročišćeni u gradijentu cezijeva klorida. Koristeći model ljudskih stanica rabdomiosarkoma, koje eksprimiraju integrin α4β1, pokazali smo povećanu djelotvornost transdukcije s Ad5VLA-4 u usporedbi s Ad5wt. Prema tome Ad5VLA-4 bi se mogao koristiti za gensku terapiju tumora koji eksprimiraju integrin α4β1.
Abstract (english)
Genetic retargeting of Adenovirus type 5 (Ad5) through insertion of sequences in the fiber protein should permit targeted gene delivery. The aim of this research is to construct replication defective adenoviral vector by incorporating α4β1 integrin-targeting ligand CPLDIDFYC into the HI-loop of the fiber protein, in order to retarget Ad5 to integrin α4β1 (also known as very late antigen 4, VLA-4). Construction of Ad5VLA-4 was performed by manipulation of the full length Ad5 genome as a stable plasmid in E. coli, using the bacterial homologous recombination machinery. Ad5VLA-4 and a control virus Ad5wt lacking targeting sequence, were multiplied in human embryonic kidney cell line-293 and purified by bending in CsCl gradients. On the rhabdomyosarcoma (RD) cell line, which express α4β1 integrin, Ad5VLA-4 exhibited increased transduction efficacy in comparison to Ad5wt. Therefore, Ad5VLA-4 could be suitable vector for gene therapy of tumors expressing α4β1 integrin.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:228201
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2018-03-12 11:11:36