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master's thesis
Mehanizam djelovanja krunastog etera 613 na stanične linije dojke
Zagreb: University of Zagreb, Faculty of Science, 2018. urn:nbn:hr:217:596076
Gabrilo, Jelena
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Gabrilo, J. (2018). Mehanizam djelovanja krunastog etera 613 na stanične linije dojke (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:596076

Gabrilo, Jelena. "Mehanizam djelovanja krunastog etera 613 na stanične linije dojke." Master's thesis, University of Zagreb, Faculty of Science, 2018. https://urn.nsk.hr/urn:nbn:hr:217:596076

Gabrilo, Jelena. "Mehanizam djelovanja krunastog etera 613 na stanične linije dojke." Master's thesis, University of Zagreb, Faculty of Science, 2018. https://urn.nsk.hr/urn:nbn:hr:217:596076

Gabrilo, J. (2018). 'Mehanizam djelovanja krunastog etera 613 na stanične linije dojke', Master's thesis, University of Zagreb, Faculty of Science, accessed 03 May 2024, https://urn.nsk.hr/urn:nbn:hr:217:596076

Gabrilo J. Mehanizam djelovanja krunastog etera 613 na stanične linije dojke [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2018 [cited 2024 May 03] Available at: https://urn.nsk.hr/urn:nbn:hr:217:596076

J. Gabrilo, "Mehanizam djelovanja krunastog etera 613 na stanične linije dojke", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:217:596076

Metadata
TitleMehanizam djelovanja krunastog etera 613 na stanične linije dojke
Title (english)Mechanism of action of crown ether compound 613 in breast cell lines
AuthorJelena Gabrilo
MentorKatja Ester (mentor)
MentorInga Marijanović (mentor)
Committee memberInga Marijanović (predsjednik povjerenstva)
Committee memberNada Oršolić (član povjerenstva)
Committee memberMartina Šeruga Musić (član povjerenstva)
Committee memberMaja Matulić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2018-02-22, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Prijašnja istraţivanja su pokazala kako krunasti eter 613 ima inhibitorni učinak na rast nekoliko tumorskih staničnih linija te da bi, s obzirom na strukturu, mogao djelovati kao mobilni nosač za ione natrija i kalija. Stoga se u sklopu ovog rada ispitivalo kako krunasti eter 613 djeluje na stanične linije tumora dojke i tumorske matične stanice dojke. Korištenjem anionske boje DiBAC4(3) utvrĎeno je da spoj značajno modulira membranski potencijal navedenih stanica, odnosno depolarizira ih. Testom MTT je pokazan i njegov antiproliferativni učinak na svim ispitivanim staničnim linijama. Kako bi se odredilo inducira li krunasti eter 613 apoptozu ili stanice umiru nekrozom, proveden je test Annexin V-FITC. Dobiveni rezultati upućuju na zaključak da je depolarizacija stanične membrane uzrokovana poremećajem homeostaze Na+, što izaziva stres za stanice na više razina te u konačnici uzrokuje smrt stanica. Ovi rezultati ukazuju na nove smjernice u istraţivanjima i primjeni krunastih etera u terapiji tumora.
Abstract (english)
Previous research has shown that crown ether compound 613 has an growth-inhibitory effect in different tumor cell lines. Considering its structure, it could act as a potassium and sodium ionophore. In this graduation thesis we tested its mechanism of action in breast tumor cell lines and breast cancer stem cell model. It was shown that the compound modulates cell membrane potential significantly, i.e. depolarizes cells. This was estimated using anionic dye DiBAC4(3). Its antiproliferative effect in tested cell lines was determined by the MTT assay. Annexin V-FITC test was performed in order to determine if cells die by apoptosis or necrosis. Our results indicate that cell membrane depolarization caused by disruption of sodium ion homeostasis leads to cellular stress and cell death eventually. These results give new directions in research and application of crown ethers in cancer therapy.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:596076
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2018-03-14 11:51:02