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master's thesis
Izražaj gena TLR9 u tumorima ljudi
Zagreb: University of Zagreb, Faculty of Science, 2009. urn:nbn:hr:217:655311
Pongrac, Igor
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Pongrac, I. (2009). Izražaj gena TLR9 u tumorima ljudi (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:655311

Pongrac, Igor. "Izražaj gena TLR9 u tumorima ljudi." Master's thesis, University of Zagreb, Faculty of Science, 2009. https://urn.nsk.hr/urn:nbn:hr:217:655311

Pongrac, Igor. "Izražaj gena TLR9 u tumorima ljudi." Master's thesis, University of Zagreb, Faculty of Science, 2009. https://urn.nsk.hr/urn:nbn:hr:217:655311

Pongrac, I. (2009). 'Izražaj gena TLR9 u tumorima ljudi', Master's thesis, University of Zagreb, Faculty of Science, accessed 23 December 2024, https://urn.nsk.hr/urn:nbn:hr:217:655311

Pongrac I. Izražaj gena TLR9 u tumorima ljudi [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2009 [cited 2024 December 23] Available at: https://urn.nsk.hr/urn:nbn:hr:217:655311

I. Pongrac, "Izražaj gena TLR9 u tumorima ljudi", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2009. Available at: https://urn.nsk.hr/urn:nbn:hr:217:655311

Metadata
TitleIzražaj gena TLR9 u tumorima ljudi
Title (english)Expression level of TLR9 gene in human tumour tissues
AuthorIgor Pongrac
MentorJasminka Pavelić (mentor)
MentorNada Oršolić (sumentor)
Committee memberJasminka Pavelić (predsjednik povjerenstva)
Committee memberNada Oršolić (član povjerenstva)
Committee memberMirjana Kalafatić (član povjerenstva)
Committee memberZlatko Liber (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2009, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Toll-like receptori (TLR) prepoznaju patogenima pridružene molekularne sljedove i omogućuju urođeni imunološki odgovor. Izraženi su i na stanicama tumora različitih tkiva što otvara mogućnost da aktivacija prijenosa signala toll-like receptorima u stanicama tumora igra značajnu ulogu u izbjegavanju imunološkog odgovora. Cilj ovog istraživanja bio je ispitati, primjenom metode real-time PCR, postoji li i koja je razina izražaja gena TLR9 u različitim vrstama tumora hipofize, te postoji li izražaj gena TLR9 u tkivima tumora pluća, i mijenja li se i kako u odnosu na zdravo tkivo. U analizu je uključeno 45 uzoraka tkiva tumora hipofize, 20 uzoraka tkiva tumora pluća i 20 uzoraka zdravog tkiva pluća. Izražaj gena TLR9 određen je korištenjem tehnologije TaqMan proba. Rezultati analize 45 uzoraka tkiva tumora hipofize pokazali su prisutnost različitih razina izražaja gena TLR9; u rasponu od izrazito visoke (2 uzorka; 4,4%) do izrazito niske (9 uzoraka; 20%). Rezultati analize 20 uzoraka tkiva tumora pluća i pripadajućih zdravih tkiva pokazali su u 8 (40%) uzoraka tkiva tumora povećan izražaj gena TLR9 u odnosu na zdravo tkivo, u 8 (40%) analiziranih uzoraka izražaj gena je bio smanjen, a u 4 uzorka (20%) nepromijenjen.
Abstract (english)
Toll-like receptors (TLRs) recognize pathogen-associated molecular patterns and enable innate immune responses. They are also expressed on tumour cells from a wide variety of tissues, suggesting that TLR activation may be an important event in tumour cell immune evasion. The aim of this study was to determine whether TLR9 expression is associated with disease manifestation in lung and hypophyseal tumours. Gene TLR9 expression was evaluated by real-time quantitative RT-PCR. Total mRNA from 45 hypophyseal tumour tissue specimen, 20 lung tumour and 20 normal lung tissue specimen was isolated, purified and retrotranscribed. cDNA was analysed measuring TLR9 relative expression using TaqMan probe technology. Results of the analysis of hypophyseal tumor tissue samples showed the presence of different levels of TLR9 gene expression: ranging from very high in 2 samples (4,4%) to distinctively low in 9 samples (20%). Results of the analysis of 20 lung tumor tissue samples and associated healthy tissues showed higher expression of TLR9 gene in 8 (40%) tumor tissues, in 8 samples (40%) the expression was decreased and in 4 samples (20%) it was unchanged, compared to healthy tissues.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:655311
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2018-03-15 10:30:41