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master's thesis
Neurodegenerativne promjene u mozgu štakora izazvane sevofluranom i željezo-dekstranom
Zagreb: University of Zagreb, Faculty of Science, 2018. urn:nbn:hr:217:201945
Pamučar, Biljana
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Pamučar, B. (2018). Neurodegenerativne promjene u mozgu štakora izazvane sevofluranom i željezo-dekstranom (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:201945

Pamučar, Biljana. "Neurodegenerativne promjene u mozgu štakora izazvane sevofluranom i željezo-dekstranom." Master's thesis, University of Zagreb, Faculty of Science, 2018. https://urn.nsk.hr/urn:nbn:hr:217:201945

Pamučar, Biljana. "Neurodegenerativne promjene u mozgu štakora izazvane sevofluranom i željezo-dekstranom." Master's thesis, University of Zagreb, Faculty of Science, 2018. https://urn.nsk.hr/urn:nbn:hr:217:201945

Pamučar, B. (2018). 'Neurodegenerativne promjene u mozgu štakora izazvane sevofluranom i željezo-dekstranom', Master's thesis, University of Zagreb, Faculty of Science, accessed 23 December 2024, https://urn.nsk.hr/urn:nbn:hr:217:201945

Pamučar B. Neurodegenerativne promjene u mozgu štakora izazvane sevofluranom i željezo-dekstranom [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2018 [cited 2024 December 23] Available at: https://urn.nsk.hr/urn:nbn:hr:217:201945

B. Pamučar, "Neurodegenerativne promjene u mozgu štakora izazvane sevofluranom i željezo-dekstranom", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:217:201945

Metadata
TitleNeurodegenerativne promjene u mozgu štakora izazvane sevofluranom i željezo-dekstranom
Title (english)Neurodegenerative changes in the rat brain induced by sevoflurane and iron-dextran
AuthorBiljana Pamučar
MentorNada Oršolić (mentor)
Committee memberNada Oršolić (predsjednik povjerenstva)
Committee memberRenata Matoničkin Kepčija (član povjerenstva)
Committee memberBiljana Balen (član povjerenstva)
Committee memberDuje Lisičić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2018-01-30, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Inhalacijski anestetici su lako hlapljive tekućine, a služe za uvođenje ili održavanje anestezije pri operativnim zahvatima nepredvidljivog trajanja. U ovom radu proučavan je utjecaj sevoflurana, samog ili združenog s željezo-dekstranom, na nastanak i razvoj neurodegenerativnih promjena na tkivu mozga štakora. Neurodegeneracija je mjerena: (i) promjenama oksidacijskog statusa mjerenjem razine malondialdehida (MDA), glutationa (GSH), te enzimatske aktivnosti katalaze (CAT) i superoksid dismutaze (SOD); (ii) (anti)genotoksičnog odgovora u limfocitima štakora, pokazujući razinu oštećenja DNA; (iii) procjenom razine neuroupale prema relativnoj težini mozga u odnosu na zdravu kontrolu; (iv) procjenom toksičnog učinka na hematološke i biokemijske odrednice u krvi, te osmotsku fragilnost eritrocita. Rezultati pokazuju da željezo-dekstran uzrokuje značajnu neuroupalu, te povećanu razinu oksidacijskog stresa: povećava razinu MDA, mijenja aktivnosti antioksidativnih enzima (SOD, CAT) i snižava GSH. Sevofluran u kombinaciji sa Fe-dekstranom pokazuje izraženi gubitak moždanih stanica i prividno niske razine MDA te antioksidansa, kao i značajne toksične učinke na krvne odrednice. Comet test pokazuje najveća oštećenja DNA limfocita pri izlaganju sevofluranu združenim sa željezo-dekstranom. Temeljem rezultata može se zaključiti kako jaka neuroupala i pojava lipidne peroksidacije u mozgu životinja obrađenih Fe-dekstranom ukazuju na neurotoksičnost, dok kombinacija sa sevofluranom uzrokuje najteža oštećenja mozga i trajno narušava homeostazu organizma.
Abstract (english)
Inhalation anaesthetics, such as sevoflurane, are commonly used in modern medicine. They are volatile gases that easily diffuse through cell membranes, used as the introduction to anaesthesia or for maintaining it in procedures of unpredictable duration. Aim of this study was to study the effects of sevoflurane, alone or in combination with Fe-dextran, on development of neurodegenerative changes in the rat brain tissue. Neurodegeneration was evaluated by: (i) (anti)oxidative status of brain tissue, by measuring the malondialdehyde (MDA) and glutathione (GSH) concentrations, catalase (CAT) and superoxide dismutase (SOD) enzymatic activities; (ii) (anti)genotoxic response in rat lymphocytes, indicating extent of DNA damage; (iii) neuroinflammation, evaluated with relative brain weight compared to healthy control; (iv) toxic effects on haematological and biochemical blood parameters, and osmotic fragility of erythrocytes. Results show that Fe-dextran causes neuroinflammation and oxidative stress: it increases MDA concentration, SOD and CAT activity and decreases GSH. Sevoflurane combined with Fe-dextran causes extensive neuroapoptosis and seemingly low MDA and antioxidant concentrations, with the highest blood toxicity and DNA damage. In summary, severe neuroinflammation and lipid peroxidation in brain of Fe-dextran treated animals indicate neurotoxicity, whereas combination with sevoflurane causes severe neurodegeneration and permanently affects whole body homeostasis.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:201945
Study programmeTitle: Experimental Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2020-01-30
Terms of useLicence In copyright icon
Created on2018-03-15 11:30:11