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master's thesis
Povezanost BRAF mutacije, histološkog gradusa i mikrosatelitske nestabilnosti s progresijom papilarnog mikrokarcinoma štitnjače
Zagreb: University of Zagreb, Faculty of Science, 2018. urn:nbn:hr:217:594754
Liber, Viktorija
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Liber, V. (2018). Povezanost BRAF mutacije, histološkog gradusa i mikrosatelitske nestabilnosti s progresijom papilarnog mikrokarcinoma štitnjače (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:594754

Liber, Viktorija. "Povezanost BRAF mutacije, histološkog gradusa i mikrosatelitske nestabilnosti s progresijom papilarnog mikrokarcinoma štitnjače." Master's thesis, University of Zagreb, Faculty of Science, 2018. https://urn.nsk.hr/urn:nbn:hr:217:594754

Liber, Viktorija. "Povezanost BRAF mutacije, histološkog gradusa i mikrosatelitske nestabilnosti s progresijom papilarnog mikrokarcinoma štitnjače." Master's thesis, University of Zagreb, Faculty of Science, 2018. https://urn.nsk.hr/urn:nbn:hr:217:594754

Liber, V. (2018). 'Povezanost BRAF mutacije, histološkog gradusa i mikrosatelitske nestabilnosti s progresijom papilarnog mikrokarcinoma štitnjače', Master's thesis, University of Zagreb, Faculty of Science, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:594754

Liber V. Povezanost BRAF mutacije, histološkog gradusa i mikrosatelitske nestabilnosti s progresijom papilarnog mikrokarcinoma štitnjače [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2018 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:217:594754

V. Liber, "Povezanost BRAF mutacije, histološkog gradusa i mikrosatelitske nestabilnosti s progresijom papilarnog mikrokarcinoma štitnjače", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:217:594754

Metadata
TitlePovezanost BRAF mutacije, histološkog gradusa i mikrosatelitske nestabilnosti s progresijom papilarnog mikrokarcinoma štitnjače
Title (english)Correlation between BRAF mutation, histological grade and microsatellite instability with progression of papillary thyroid microcarcinoma
AuthorViktorija Liber
MentorFabijan Knežević (mentor)
MentorVesna Benković (mentor)
Committee memberVesna Benković (predsjednik povjerenstva)
Committee memberBiljana Balen (član povjerenstva)
Committee memberGoran Kovačević (član povjerenstva)
Committee memberRenata Šoštarić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2018-02-22, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Papilarni mikrokarcinom štitnjače (PTMC) definira se kao tumor manji od 1 cm koji je građen od resica obloženim atipičnim tireocitima. PTMC većinom ima dobru prognozu, no neki PTMC-ovi pokazuju intraglandularnu diseminaciju, metastaze u regionalnim limfnim čvorovima vrata, a rijetko i udaljene metastaze. Cilj ovog istraživanja bio je ustanoviti postoji li povezanost proliferacijskog indeksa Ki-67 (histološki gradus), mikrosatelitske nestabilnosti (MSI), i mutacije BRAF kod PTMC-a s progresijom tumorske bolesti. Imunohistokemijom se na 41 uzorku biopsija tumora PTMC-a odredilo prisustvo mutacije u proteinu BRAF te postojanje MSI, a ostali podaci su preuzeti iz patohistoloških nalaza arhive Zavoda za patologiju i citologiju KB "Sveti Duh". Svi podaci analizirani su deskriptivnom statistikom i neparametrijskim korelacijskim testom Spearman. Bolesnici stariji od 50 godina imali su izraženiji protein MLH1. Metastaze u limfne čvorove vrata pojavile su se češće kod većih tumora. Kod nižih vrijednosti indeksa Ki-67 tumor je češće pokazivao mikrosatelitsku nestabilnost. Mutacija BRAF češće je detektirana u mlađih bolesnika, kod tumora histološkog gradusa 2 te kod većih tumora. Može se zaključiti da je veličina PTMC-a povezana s mutacijom BRAF, MSI te pojavom metastaza.
Abstract (english)
Papillary thyroid microcarcinoma is defined as tumor measuring less than 1 cm; it consists of papillae which are coated with atypical thyreocytes. PTMC predominantly has a good prognosis, but some cases show intraglandular dissemination, metastasis in regional lymph node and rarely distant metastasis. The aim of this study was to determine whether there is a correlation between the proliferation index Ki-67 (histological grade), microsatellite instability (MSI) and BRAF mutation in PTMC, with progression of tumor disease. Immunohistochemistry was used to determine the presence of BRAF mutated proteins and the existence of MSI in the 41 biopsy samples of the PTMC tumor, rest of the data were taken from the pathohistological findings in the archives of the Department of Pathology and Cytology "Sveti Duh". All data were analyzed by descriptive statistics and nonparametric Spearman correlation test. Patients over the age of 50 had more expressed MLH1 protein. Metastases in lymph nodes appeared more frequently in larger tumors. At lower values of the Ki-67 index the tumor showed microsatellite instability. Most BRAF mutations were detected in younger patients, in patients with histological tumor grade 2 and larger tumors. We can conclude that the size of PTMC is associated with BRAF mutations, MSI and the occurrence of metastasis.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:594754
Study programmeTitle: Experimental Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2018-03-15 13:10:27