Concentration of total coliform bacteria from surface water in area of city of Zagreb, was investigated on sample of clean culture of Escherichia coli and 19 samples of water, collected at the lake of Botanical Garden, five lakes of Maksimir and at seven brooks of Zagreb. Four commercial solid media were used for cultivation of total coliform bacteria, and they are: ECX Gluc agar, Endo agar, m-Faecal Coliform agar and MacConkey agar. Samples was inoculated on each media with Plate Strake Method. The referred number of total coliform bacteria and the percentage of E.coli in the population of total coliform in the same sample was significantly different on various media. Comparing the dana optained from counting down overgrown colonies of coliform bacteria, was established that the concentratoin of total coliform bacteria differ according to the sample of the primary location. Their biggest concentration was determined in nonstagnant waters, which primarily includes brook of Vugrov, Vrapčak, Črnomerec and Srednjak. Lower concentration of total coliform bacteria was determined in the lake of Botanic garden, the Fifth lake of Maksimir and in the brooks of Maksimir, Bliznec and Maksimirec. The water quality was ratified on the base of the number of total coliform bacteria and the Water classification regulation of the Republic of Croatia (˝Narodne novine˝ 77/98). The V class of water was defined in 6 out of 19 analysed samples, whereas other samples showed the IV class of water.