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master's thesis
Vezanje proteina CTCF i BORIS na promotor gena TOP2A u stanicama Cal 27
Zagreb: University of Zagreb, Faculty of Science, 2009. urn:nbn:hr:217:874330
Ćuk, Katarina
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Ćuk, K. (2009). Vezanje proteina CTCF i BORIS na promotor gena TOP2A u stanicama Cal 27 (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:874330

Ćuk, Katarina. "Vezanje proteina CTCF i BORIS na promotor gena TOP2A u stanicama Cal 27." Master's thesis, University of Zagreb, Faculty of Science, 2009. https://urn.nsk.hr/urn:nbn:hr:217:874330

Ćuk, Katarina. "Vezanje proteina CTCF i BORIS na promotor gena TOP2A u stanicama Cal 27." Master's thesis, University of Zagreb, Faculty of Science, 2009. https://urn.nsk.hr/urn:nbn:hr:217:874330

Ćuk, K. (2009). 'Vezanje proteina CTCF i BORIS na promotor gena TOP2A u stanicama Cal 27', Master's thesis, University of Zagreb, Faculty of Science, accessed 23 December 2024, https://urn.nsk.hr/urn:nbn:hr:217:874330

Ćuk K. Vezanje proteina CTCF i BORIS na promotor gena TOP2A u stanicama Cal 27 [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2009 [cited 2024 December 23] Available at: https://urn.nsk.hr/urn:nbn:hr:217:874330

K. Ćuk, "Vezanje proteina CTCF i BORIS na promotor gena TOP2A u stanicama Cal 27", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2009. Available at: https://urn.nsk.hr/urn:nbn:hr:217:874330

Metadata
TitleVezanje proteina CTCF i BORIS na promotor gena TOP2A u stanicama Cal 27
Title (english)Binding of CTCF and BORIS to TOP2A promoter in Cal 27 cells
AuthorKatarina Ćuk
MentorRenata Novak Kujundžić (mentor)
MentorNada Oršolić (sumentor)
Committee memberNada Oršolić (predsjednik povjerenstva)
Committee memberRenata Novak Kujundžić (član povjerenstva)
Committee memberMaja Matulić (član povjerenstva)
Committee memberGordana Rusak (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2009, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Topoizomeraza IIα (kodirana genom TOP2A) je enzim neophodan za preživljavanje stanica. Razina tog enzima je često povišena u zloćudnim tumorima i povezuje se sa osjetljivošću stanica na citotoksične lijekove. Protein CTCF i njegov paralog BORIS, koji se ispoljava samo u subpopulaciji spermatocita te aberantno u brojnim tumorima, imaju važnu ulogu u uspostavljanju i održavanju metilacije DNK i regulaciji transkripcije brojnih gena. S obzirom da se ova dva proteina mogu vezati na ista mjesta u genomu, ali imaju različitu ulogu u regulaciji transkripcije, cilj ovog rada bio je utvrditi da li se oni vežu na promotor gena TOP2A. Tretmanom 3- aminobenzamidom sam potaknula ispoljavanje proteina BORIS u stanicama Cal 27 te metodom kromatinske imunoprecipitacije pokazala da se proteini CTCF i BORIS vežu na navedeni promotor. Rezultati ovog istraživanja će olakšati upoznavanje uloge ovih proteina u regulaciji transkripcije gena TOP2A i primjenjivosti njezine modulacije u nalaženju novih pristupa u liječenju tumora.
Abstract (english)
The enzyme topoisomerase IIα (encoded by TOP2A) is essential for cell survival. Its expression is often upregulated in malignant tumors and has been implicated in cellular sensitivity to cytotoxic drugs. Protein CTCF and its paralogue BORIS, which is normally expressed only in a subpopulation of spermatocytes and aberrantly in various tumors, have important roles in the establishment and maintenance of DNA methylation and transcriptional regulation. Since those two proteins bind to the same DNA motifs, but have different roles in transcriptional regulation, the aim of this study was to investigate their association with the TOP2A promoter. With an 3-aminobenzamide treatment I have induced BORIS in Cal 27 cells and by chromatin immunoprecipitation demonstrated that CTCF and BORIS bind to the TOP2A promoter. Results of this study facilitate further investigation of their role in the regulation of TOP2A transcription and the applicability of its modulation in the therapy of malignant tumors.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:874330
Study programmeTitle: Biology; specializations in: Molecular biology
Course: Molecular biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra biologije (magistar/magistra biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2018-03-20 13:32:40