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master's thesis
Zakorjenjivanje i aklimatizacija crnoplodne aronije (Aronia melanocarpa Michx.) razmnožene u uvjetima in vitro
Zagreb: University of Zagreb, Faculty of Science, 2009. urn:nbn:hr:217:690919
Črnjević, Silvija
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Črnjević, S. (2009). Zakorjenjivanje i aklimatizacija crnoplodne aronije (Aronia melanocarpa Michx.) razmnožene u uvjetima in vitro (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:690919

Črnjević, Silvija. "Zakorjenjivanje i aklimatizacija crnoplodne aronije (Aronia melanocarpa Michx.) razmnožene u uvjetima in vitro." Master's thesis, University of Zagreb, Faculty of Science, 2009. https://urn.nsk.hr/urn:nbn:hr:217:690919

Črnjević, Silvija. "Zakorjenjivanje i aklimatizacija crnoplodne aronije (Aronia melanocarpa Michx.) razmnožene u uvjetima in vitro." Master's thesis, University of Zagreb, Faculty of Science, 2009. https://urn.nsk.hr/urn:nbn:hr:217:690919

Črnjević, S. (2009). 'Zakorjenjivanje i aklimatizacija crnoplodne aronije (Aronia melanocarpa Michx.) razmnožene u uvjetima in vitro', Master's thesis, University of Zagreb, Faculty of Science, accessed 20 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:690919

Črnjević S. Zakorjenjivanje i aklimatizacija crnoplodne aronije (Aronia melanocarpa Michx.) razmnožene u uvjetima in vitro [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2009 [cited 2024 November 20] Available at: https://urn.nsk.hr/urn:nbn:hr:217:690919

S. Črnjević, "Zakorjenjivanje i aklimatizacija crnoplodne aronije (Aronia melanocarpa Michx.) razmnožene u uvjetima in vitro", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2009. Available at: https://urn.nsk.hr/urn:nbn:hr:217:690919

Metadata
TitleZakorjenjivanje i aklimatizacija crnoplodne aronije (Aronia melanocarpa Michx.) razmnožene u uvjetima in vitro
Title (english)Rooting and acclimatization of black chokeberry (Aronia melanocarpa Michx.) shoots
AuthorSilvija Črnjević
MentorBranka Pevalek-Kozlina (mentor)
MentorMarija Babić (pomoć pri izradi, neposredni voditelj)
Committee memberBranka Pevalek-Kozlina (predsjednik povjerenstva)
Committee memberDubravka Matković-Čalogović (član povjerenstva)
Committee memberBiserka Prugovečki (član povjerenstva)
Committee memberInes Radanović (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2009, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Crnoplodna aronija (Aronia melanocarpa Michx.) je listopadni grm iz porodice Rosaceae. Zbog izvanrednih antioksidacijskih svojstava plodova, vrsta je uvedena u kulturu in vitro. U ovom radu prikazani su rezultati istraživanja zakorjenjivanja i aklimatizacije crnoplodne aronije na hranjivoj podlozi MS s različitim koncentracijama makroelemenata (MS, ½ MS i ¼ MS). Najveća stopa zakorjenjivanja (85,4%) te najbolje morfološke značajke biljaka postignute su na podlozi ½ MS. Istraživanje je nastavljeno na ½ MS uz dodatak auksina α- naftalenoctene kiseline (NAA) i indol-3-maslačne kiseline (IBA). Dodani auksini inhibirali su stvaranje primarnog te potaknuli stvaranje adventivnog korijenja iz prethodno nastalog kalusnog tkiva. Na podlozi s 1,5 mg/L NAA stopa adventivnog zakorjenjivanja iznosila je 95,8%, a na podlozi s 1,0 mg/L IBA postotak adventivnog zakorjenjivanja iznosio je 100%. Aklimatizacija in vitro zakorjenjenih biljaka bila je podjednako uspješna (u 80-90% biljka) na komercijalnom (COMPO SANA) i na pripremljenom supstratu (mješavina pumice, tenisit i treseta u omjeru 3:3:1). Zakorjenjivanje izdanaka ex vitro provedeno je na istim supstratima uz istovremenu aklimatizaciju, te se pokazalo manje uspješnim (u 50-60% biljaka) od aklimatizacije biljaka prethodno zakorjenjenih u uvjetima in vitro (u 80-90% biljka).
Abstract (english)
Black chokeberry (Aronia melanocarpa Michx.) is a deciduous bush-like plant which belongs to the Rosaceae family. The species was introduced in in vitro culture due to high atioxidative activity of their fruits. Rooting and subsequent acclimatization of black chokeberry has been investigated on MS nutrient media containing different concentration of macronutrients (MS, ½ MS, ¼ MS). The highest rooting percentage (85.4%) and the best morphological characteristics were obtained on ½ MS medium. Therefore, the research was continued on ½ MS, supplemented with auxins α- naphthaleneacetic acid (NAA) or indole-3-butyric acid (IBA). Auxins NAA or IBA inhibited the main root induction, but stimulated adventitious root formation from the callus. Maximum adventitious rooting occured in shoots grown on ½ MS containing 1.5 mg/L NAA (95.8%) or 1.0 mg/L IBA (100%). Acclimatization of in vitro rooted microcuttings was equally successful (80-90%) in plants potted to commercial peat substrate (COMPO SANA) and mixture of pumice, tenisit and peat (3:3:1). Aronia melanocarpa microcuttings without roots exhibited poor ex vitro rooting and low acclimatization percentage (50-60%).
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:690919
Study programmeTitle: Biology and Chemistry
Study programme type: university
Study level: graduate
Academic / professional title: profesor biologije i kemije (profesor biologije i kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2018-03-21 08:53:24