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master's thesis
Određivanje toksičnosti uzoraka sedimenta potoka Gorjak biotestovima in vitro
Zagreb: University of Zagreb, Faculty of Science, 2009. urn:nbn:hr:217:311475
Šestak, Ivana
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Šestak, I. (2009). Određivanje toksičnosti uzoraka sedimenta potoka Gorjak biotestovima in vitro (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:311475

Šestak, Ivana. "Određivanje toksičnosti uzoraka sedimenta potoka Gorjak biotestovima in vitro." Master's thesis, University of Zagreb, Faculty of Science, 2009. https://urn.nsk.hr/urn:nbn:hr:217:311475

Šestak, Ivana. "Određivanje toksičnosti uzoraka sedimenta potoka Gorjak biotestovima in vitro." Master's thesis, University of Zagreb, Faculty of Science, 2009. https://urn.nsk.hr/urn:nbn:hr:217:311475

Šestak, I. (2009). 'Određivanje toksičnosti uzoraka sedimenta potoka Gorjak biotestovima in vitro', Master's thesis, University of Zagreb, Faculty of Science, accessed 27 December 2024, https://urn.nsk.hr/urn:nbn:hr:217:311475

Šestak I. Određivanje toksičnosti uzoraka sedimenta potoka Gorjak biotestovima in vitro [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2009 [cited 2024 December 27] Available at: https://urn.nsk.hr/urn:nbn:hr:217:311475

I. Šestak, "Određivanje toksičnosti uzoraka sedimenta potoka Gorjak biotestovima in vitro", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2009. Available at: https://urn.nsk.hr/urn:nbn:hr:217:311475

Metadata
TitleOdređivanje toksičnosti uzoraka sedimenta potoka Gorjak biotestovima in vitro
Title (english)Determination of sediment toxicity of Gorjak stream by bioassays in vitro
AuthorIvana Šestak
MentorTvrtko Smital (mentor)
MentorGoran Klobučar (mentor)
Committee memberTvrtko Smital (predsjednik povjerenstva)
Committee memberGoran Klobučar (član povjerenstva)
Committee memberMirjana Pavlica (član povjerenstva)
Committee memberŽeljka Vidaković Cifrek (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2009, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Brojna teško razgradiva organska zagađivala (eng. Persistent Organic Pollutants; POPs) poput polikloriranih dibenzo-p-dioksina i furana (PCDD/F), polikloriranih bifenila (PCB), polikloriranih naftalena (PCN) i policikličkih aromatskih ugljikovodika (PAH) izrazito su toksična. POP-sovi u okoliš najčešće dospijevaju industrijskim otpadnim vodama. Ispuštanjem nepročišćenih industrijskih otpadnih voda u potok Gorjak, tvrtke Pliva Hrvatska d.d. i Kvasac d.o.o. desetljećima predstavljaju problem za širu zajednicu. Cilj ovog istraživanja bio je odrediti razinu toksičnosti sedimenta potoka Gorjak pomoću dva biotesta: (1) testa kronične toksičnosti (inhibicija rasta zelene alge Scenedesmus subspicatus); te (2) EROD testa određivanja aktivnosti staničnog detoksikacijskog sustava oksidaza miješanih funkcija. Testom kronične toksičnosti mjerili smo razrjeđenja uzoraka sedimenta pri kojima je dosegnuta 50%-tna inhibicije rasta alge S. subspicatus (IC50) u odnosu na kontrolnu grupu. Odredili smo EC50 vrijednosti koje predstavljaju ekvivalentnu količinu sedimenta, te izračunali toksične jedinice (TJ) dobivene prema izrazu TJ = 100 / EC50. Izvođenjem EROD testa izračunali smo TCDD toksične ekvivalente (TCDD TEQ). Rezultati pokazuju visoku toksičnost sedimenata na svim lokacijama, uz izrazito visoke vrijednosti neposredno nakon ispusta industrijskih otpadnih voda spomenutih tvrtki.
Abstract (english)
Numerous persistent organic pollutants (POPs), such as polychlorinated dibenzo-p-dioxins and furans (PCDD/F), polychlorinated biphenyls (PCBs), polychlorinated naphthalenes (PCNs) and polyaromatic hydrocarbons (PAHs) are highly toxic. POPs get into environment mostly by industrial wastewaters. By releasing untreated industrial wastewater into the Gorjak stream, Industries Pliva Hrvatska d.d. and Kvasac d.o.o. for decades represent a problem for local community. The purpose of this research was to determine ecotoxicity of Gorjak stream sediment samples, using two bioassays: (1) chronic toxicity test (growth inhibition test with green algae Scenedesmus subspicatus), and (2) EROD test for determination of the activity of the mixed function oxygenases cellular detoxification system. Using chronic toxicity test we measured sample dilutions at which 50% growth inhibion of the algae was achieved (IC50) in comparison to the control group. EC50 values representing corresponding amount of sediment were determined and toxicity units (TU) obtained recalculated by expression TU = 100 / EC50. For the EROD bioassay we calculated TCDD toxicity equivalents (TCDD TEQ). Results showed high sediment toxicity at each location, with the highest values determined at location closest to the wastewater outlet of the above mentioned industrial facilities.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:311475
Study programmeTitle: Biology; specializations in: Ecology
Course: Ecology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra biologije (magistar/magistra biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2018-03-21 09:19:01