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master's thesis
Uloga gena HOXB13 i njegovih supstrata u progresiji karcinoma jajnika
Zagreb: University of Zagreb, Faculty of Science, 2009. urn:nbn:hr:217:118361
Šuštić, Tonći
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Šuštić, T. (2009). Uloga gena HOXB13 i njegovih supstrata u progresiji karcinoma jajnika (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:118361

Šuštić, Tonći. "Uloga gena HOXB13 i njegovih supstrata u progresiji karcinoma jajnika." Master's thesis, University of Zagreb, Faculty of Science, 2009. https://urn.nsk.hr/urn:nbn:hr:217:118361

Šuštić, Tonći. "Uloga gena HOXB13 i njegovih supstrata u progresiji karcinoma jajnika." Master's thesis, University of Zagreb, Faculty of Science, 2009. https://urn.nsk.hr/urn:nbn:hr:217:118361

Šuštić, T. (2009). 'Uloga gena HOXB13 i njegovih supstrata u progresiji karcinoma jajnika', Master's thesis, University of Zagreb, Faculty of Science, accessed 23 December 2024, https://urn.nsk.hr/urn:nbn:hr:217:118361

Šuštić T. Uloga gena HOXB13 i njegovih supstrata u progresiji karcinoma jajnika [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2009 [cited 2024 December 23] Available at: https://urn.nsk.hr/urn:nbn:hr:217:118361

T. Šuštić, "Uloga gena HOXB13 i njegovih supstrata u progresiji karcinoma jajnika", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2009. Available at: https://urn.nsk.hr/urn:nbn:hr:217:118361

Metadata
TitleUloga gena HOXB13 i njegovih supstrata u progresiji karcinoma jajnika
Title (english)HOXB13 and its downstream targets in ovarian cancer progression
AuthorTonći Šuštić
MentorNada Oršolić (mentor)
Committee memberGordana Lacković-Venturin (predsjednik povjerenstva)
Committee memberMaja Matulić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2009, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
HOX geni se povezuju s različitim aspektima neoplastične progresije, budući da ektotopsko izražavanje nekih HOX gena promovira staničnu transformaciju in vitro i tumorogenezu in vivo. Pojačano izražavanje gena HOXB13 primijećeno je kod visokog postotka tumora dojke i jajnika te se povezuje s izrazito agresivnim kliničkim tijekom. Početni cilj ovog istraživanja je ispitati utjecaj ektotopskog izražavanja gena HOXB13 u genetički definiranim modelnim staničnim linijama karcinoma jajnika. Western analizama posredno je identificirano nekoliko gena čije je izražavanje u visokoj pozitivnoj ili negativnoj korelaciji s izražavanjem gena HOXB13. Među ovim genima, posebno se istaknuo klasterin, gen čiji se proteinski produkt (CLU) javlja u dvije izoforme. sCLU posjeduje signalnu sekvencu za sekreciju van stanice te mu se pripisuje citoprotektivna uloga, dok je nCLU lokaliziran u citoplazmi, a u slučaju oštećenja stanice odlazi u jezgru gdje obavlja proapoptotsku ulogu. Kao dio projekta identifikacije novih molekularnih meta koje bi bile podložne terapeutskoj intervenciji u karcinomu jajnika, ovaj rad opisuje proces dobivanja dviju novih staničnih linija koje predstavljaju primjere međusobno disjunktnih uloga klasterina. Dobivene modelne linije analizirane su in vitro i in vivo, te je dokazano stabilno ektotopsko izražavanje obiju klasterinskih izoformi.
Abstract (english)
The HOX genes have been implicated in different aspects of the oncogenic process, as ectopic expression of some HOX genes promotes cellular transformation in vitro and tumorigenesis in vivo. The observation that HOXB13 is expressed in a high percentage of breast and ovarian cancer cell lines and tumors, and that the expression of HOXB13 is associated with a more aggressive clinical course, led us to investigate the ectopic expression of HOXB13 in genetically defined ovarian cancer cell lines. We report identification of several putative HOXB13 targets among which clusterin takes the most prominent role. Clusterin is present in two distinct isoforms, as secreted clusterin (sCLU) and as nuclear clusterin (nCLU). While the former is associated with cell survival, the latter is pro-apoptotic. As a part of the project aiming to identify new therapeutic targets for ovarian cancer, here we describe the generation of clusterinoverexpressing cell lines, representing two contradictory functions of clusterin. These cell lines have subsequently been analyzed using both in vitro and in vivo assays. Consequently, stable ectopic expression of both clusterin isoforms has been demonstrated.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:118361
Study programmeTitle: Biology; specializations in: Molecular biology
Course: Molecular biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra biologije (magistar/magistra biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2018-03-21 12:53:04