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master's thesis
Kompleksi lipida i DNK
Zagreb: University of Zagreb, Faculty of Science, 2016. urn:nbn:hr:217:948850
Poljak, Frano
University of Zagreb
Faculty of Science
Department of Physics

Institutional repository: Repository of the Faculty of Science

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Poljak, F. (2016). Kompleksi lipida i DNK (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:948850

Poljak, Frano. "Kompleksi lipida i DNK." Master's thesis, University of Zagreb, Faculty of Science, 2016. https://urn.nsk.hr/urn:nbn:hr:217:948850

Poljak, Frano. "Kompleksi lipida i DNK." Master's thesis, University of Zagreb, Faculty of Science, 2016. https://urn.nsk.hr/urn:nbn:hr:217:948850

Poljak, F. (2016). 'Kompleksi lipida i DNK', Master's thesis, University of Zagreb, Faculty of Science, accessed 23 December 2024, https://urn.nsk.hr/urn:nbn:hr:217:948850

Poljak F. Kompleksi lipida i DNK [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2016 [cited 2024 December 23] Available at: https://urn.nsk.hr/urn:nbn:hr:217:948850

F. Poljak, "Kompleksi lipida i DNK", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2016. Available at: https://urn.nsk.hr/urn:nbn:hr:217:948850

Metadata
TitleKompleksi lipida i DNK
Title (english)Lipids-DNA complexes
AuthorFrano Poljak
MentorTomislav Vuletić (mentor)
MentorDamir Bosnar (mentor)
Committee memberDamir Bosnar (predsjednik povjerenstva)
Committee memberTomislav Vuletić (član povjerenstva)
Committee memberMatko Glunčić (član povjerenstva)
Committee memberNenad Pavin (član povjerenstva)
Committee memberMario Basletić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Physics)
Zagreb
Defense date and country2016-02-22, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Physics
Abstract
Sastavljen je mjerni uređaj za fluorescencijsku korelacijsku spektroskopiju i kalibriran je koristeći se poznatim parametrima fluorofore Cy5. Određen je efektivni konfokalni volumen. Odredeni su koeficijenti difuzije DNK15 i DNK60 molekula, eksperimentalno i prema teorijskom modelu. Kao test reproducibilnosti uređaja provedeno je mjerenje ovisnosti difuzijskog vremena DNK15 o viskoznosti okolnog medija. Dobiveni rezultati dobro reproduciraju očekivanu ovisnost. Sintetizirani su različiti liposomski uzorci te su provedena mjerenja autokorelacija fluorescentno označenih DNK pomiješanih s liposomima u svrhu opažanja kompleksacije DNK s lipidima. Nestabilni lipopleksi opaženi su pri miješanju DNK15 s kationskim liposomima, dok kompleksacija nije uočena pri dodavanju liposoma s ugrađenim adamantan-aminogvanidinom. Opsežniji eksperimenti trebaju biti provedeni u svrhu unapređivanja mjernog uređaja i proučavanja liposomske transfekcije u genskoj terapiji.
Abstract (english)
Using known parameters for Cy5 fluorophore, a fluorescence correlation spectroscopy instrument was installed. The effective confocal volume was determined. Diffusion coefficients of DNA15 and DNA60 molecules were determined, both experimentally and according to the theoretical model. As a reproducibility test, the diffusion time versus medium viscosity measurement for DNA15 was conducted. The results show a good agreement with the expected dependency. A various liposome samples were synthesized and the autocorrelation measurements of a fluorescently labelled DNA mixed with liposomes were performed, in order to detect their complexation. When cationic liposomes were added, unstable lipoplexes were detected, while complexation was not observed in case of adamantyl aminoguanidineincorporated liposomes. Further experiments need to be conducted to improve the instrument and to better understand the liposome transfection in gene therapy.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:948850
Study programmeTitle: Physics; specializations in: Research
Course: Research
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: magistar/magistra fizike (magistar/magistra fizike)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-03-05 10:57:31