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master's thesis
Produkcija onkoproteina E6 i E7 humanog papiloma virusa u bakteriji Escherichia coli
Zagreb: University of Zagreb, Faculty of Science, 2010. urn:nbn:hr:217:386600
Jerković, Ivana
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Jerković, I. (2010). Produkcija onkoproteina E6 i E7 humanog papiloma virusa u bakteriji Escherichia coli (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:386600

Jerković, Ivana. "Produkcija onkoproteina E6 i E7 humanog papiloma virusa u bakteriji Escherichia coli." Master's thesis, University of Zagreb, Faculty of Science, 2010. https://urn.nsk.hr/urn:nbn:hr:217:386600

Jerković, Ivana. "Produkcija onkoproteina E6 i E7 humanog papiloma virusa u bakteriji Escherichia coli." Master's thesis, University of Zagreb, Faculty of Science, 2010. https://urn.nsk.hr/urn:nbn:hr:217:386600

Jerković, I. (2010). 'Produkcija onkoproteina E6 i E7 humanog papiloma virusa u bakteriji Escherichia coli', Master's thesis, University of Zagreb, Faculty of Science, accessed 25 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:386600

Jerković I. Produkcija onkoproteina E6 i E7 humanog papiloma virusa u bakteriji Escherichia coli [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2010 [cited 2024 November 25] Available at: https://urn.nsk.hr/urn:nbn:hr:217:386600

I. Jerković, "Produkcija onkoproteina E6 i E7 humanog papiloma virusa u bakteriji Escherichia coli", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2010. Available at: https://urn.nsk.hr/urn:nbn:hr:217:386600

Metadata
TitleProdukcija onkoproteina E6 i E7 humanog papiloma virusa u bakteriji Escherichia coli
Title (english)Production of human papilloma virus E6 and E7 oncoproteins in Escherichia coli
AuthorIvana Jerković
MentorMagdalena Grce (mentor)
MentorMaja Matulić (sumentor)
Committee memberMagdalena Grce (predsjednik povjerenstva)
Committee memberMaja Matulić (član povjerenstva)
Committee memberGordana Lacković-Venturin (član povjerenstva)
Committee memberMirna Ćurković-Perica (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2010, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Humani papiloma virus (HPV) spada u obitelj Papilomavirusa. U 1970‐ima dokazano je kako infekcija čovjeka ovim virusom kod žena može dovesti do razvoja raka vrata maternice. Za onkogenična svojstva HPV‐a odgovorni su E6 i E7 geni, odnosno njihovi proteini. Cilj ovog istraživanja bio je eksprimirati E6 i E7 gene tako da dobijemo nativne i topive proteine koji se mogu koristiti za daljnje istraživanje. Uvidom u slijed nukleotida genoma HPV 16 odabrane su početnice za umnažanje E6 i E7 gena. Pomoću vektorskog sustava prenešeni su u bakteriji E. coli gdje se odvijala ekspresija. U ovom radu smo, mijenjali uvjete ekspresije kako bi našli optimalne za dobivanje nativnih proteina. Iako je ekspresija bila uspješna, nijedan od promijenjenih parametara nije utjecao na topivost proteina. Kao alternativni pristup, proteine smo izolirali u denaturirajućim uvjetima.
Abstract (english)
Human papilloma virus (HPV) belongs to Papillomavirus family. In the 1970s it has been demonstrated that human infection by this virus in women may lead to the development of cervical cancer. The oncogenic properties of HPV are E6 and E7 genes. The aim of this study was the expression of E6 and E7 genes in order to obtain native, productive proteins for further research. Based on the genome sequence of HPV type 16, specific primers for E6 and E7 amplification were designed. Using a vector system the amplicon were transferred into E. coli bacteria where they were expressed. In this study, we have changed the conditions of the expression in order to find the optimal condition. Although the expression was successful, the proteins remained unsoluble under every tested change. Therefore, the alternative approach that we applied was to extract the proteins in denaturating conditions.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:386600
Study programmeTitle: Biology; specializations in: Molecular biology
Course: Molecular biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra biologije (magistar/magistra biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2018-03-23 13:11:13