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master's thesis
Protutumorski učinak kombinirane primjene dvolančane RNA i kemoterapeutika
Zagreb: University of Zagreb, Faculty of Science, 2010. urn:nbn:hr:217:275171
Kirinec, Gabriela
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Kirinec, G. (2010). Protutumorski učinak kombinirane primjene dvolančane RNA i kemoterapeutika (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:275171

Kirinec, Gabriela. "Protutumorski učinak kombinirane primjene dvolančane RNA i kemoterapeutika." Master's thesis, University of Zagreb, Faculty of Science, 2010. https://urn.nsk.hr/urn:nbn:hr:217:275171

Kirinec, Gabriela. "Protutumorski učinak kombinirane primjene dvolančane RNA i kemoterapeutika." Master's thesis, University of Zagreb, Faculty of Science, 2010. https://urn.nsk.hr/urn:nbn:hr:217:275171

Kirinec, G. (2010). 'Protutumorski učinak kombinirane primjene dvolančane RNA i kemoterapeutika', Master's thesis, University of Zagreb, Faculty of Science, accessed 25 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:275171

Kirinec G. Protutumorski učinak kombinirane primjene dvolančane RNA i kemoterapeutika [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2010 [cited 2024 November 25] Available at: https://urn.nsk.hr/urn:nbn:hr:217:275171

G. Kirinec, "Protutumorski učinak kombinirane primjene dvolančane RNA i kemoterapeutika", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2010. Available at: https://urn.nsk.hr/urn:nbn:hr:217:275171

Metadata
TitleProtutumorski učinak kombinirane primjene dvolančane RNA i kemoterapeutika
Title (english)Antitumor activity of combined application of double stranded RNA and chemotherapeutics
AuthorGabriela Kirinec
MentorJasminka Pavelić (mentor)
Committee memberJasminka Pavelić (predsjednik povjerenstva)
Committee memberMaja Matulić (član povjerenstva)
Committee memberMirta Tkalec (član povjerenstva)
Committee memberDomagoj Đikić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2010, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Toll-like receptor 3, uz ostale receptore "Toll-like" (TLR, od engl. Toll-like receptors), ima ključnu ulogu u urođenoj imunosti. Nedavna istraživanja pokazala su da se TLR3, kao i ostali srodni receptori, nalazi izražen i u stanicama određenih vrsta tumora, te da njegova aktivacija potiče apotozu stanica tumora i inhibira njihovu proliferaciju. Također je otkriveno da aktivacija TLR3 u kombinaciji s protutumorskim terapeuticima povećava citotoksični učinak agonista TLR3 na stanice tumora. To dovodi do zaključka da se agonisti TLR3 mogu koristiti kao protutumorski agensi zbog svojeg citostatskog i citotoksičnog djelovanja na stanice tumora. U ovom istraživanju ispitali smo djelovanje liganda TLR3, poliriboinozilne:poliribocitidilne kiseline (poly(I:C)), u kombinaciji s kemoterapeuticima 5-fluorouracilom i metotreksatom, na stanice primarnog i metastatskog tumora ždrijela. Svrha ovog rada jest ispitati mogućnost kombinirane primjene poly(I:C) i kemoterapeutika kao protutumorske terapije. Prema dobivenim rezultatima ustanovili smo da kombinirana primjena poly(I:C) i kemoterapeutika ima značajno veći inhibitorni učinak na stanice nego zasebni tretman s poly(I:C), tj. da poly(I:C) i kemoterapeutici 5-fluorouracil i metotreksat djeluju sinergistički. Također smo utvrdili da je inhibitorni učinak kombiniranog tretmana s poly(I:C) i kemoterapeuticima posljedica poly(I:C)-aktivirane apoptoze ovisne o TLR3-signalnom putu.
Abstract (english)
Toll-like receptor 3, as well as other TLRs, plays a key role in innate immunity. Recent studies have shown that TLR3 is expressed on certain types of tumor cells and that its activation stimulates apotosis of tumor cells and inhibits their proliferation. Also it has been found that activation of TLR3 in combination with antitumor therapeutics increases the cytotoxic effect of TLR3 agonist on tumor cells. This leads to the conclusion that the TLR3 agonists can be used as antitumor agents because of their citostatic and cytotoxic effects on tumor cells. In this study, we investigated the effect of TLR3 ligand, polyinosinic:polycytidylic acid (poly(I:C)), in combination with chemotherapeutics 5-fluorouracil and methotrexate on primary and metastatic pharyngeal carcinoma cell lines. The purpose of this study was to examine the possibility of combined application of poly(I:C) and chemotherapeutics as an antitumor therapy. We have found that combined application of poly(I:C) and chemotherapeutics has a significantly greater inhibitory effect on cell growth than the separate treatment with poly(I:C), i.e. poly(I:C) and chemotherapeutics act synergistically. We also found that the inhibitory effect of combined treatment with poly(I:C) and chemotherapeutics is a result of TLR3-dependent apoptosis.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:275171
Study programmeTitle: Biology; specializations in: Molecular biology
Course: Molecular biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra biologije (magistar/magistra biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2018-03-23 13:53:18