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master's thesis
Umnažanje ljudskih mezenhimskih matičnih stanica in vitro u prisustvu lizata trombcita
Zagreb: University of Zagreb, Faculty of Science, 2010. urn:nbn:hr:217:616081
Klanfar, Tomislav
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Klanfar, T. (2010). Umnažanje ljudskih mezenhimskih matičnih stanica in vitro u prisustvu lizata trombcita (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:616081

Klanfar, Tomislav. "Umnažanje ljudskih mezenhimskih matičnih stanica in vitro u prisustvu lizata trombcita." Master's thesis, University of Zagreb, Faculty of Science, 2010. https://urn.nsk.hr/urn:nbn:hr:217:616081

Klanfar, Tomislav. "Umnažanje ljudskih mezenhimskih matičnih stanica in vitro u prisustvu lizata trombcita." Master's thesis, University of Zagreb, Faculty of Science, 2010. https://urn.nsk.hr/urn:nbn:hr:217:616081

Klanfar, T. (2010). 'Umnažanje ljudskih mezenhimskih matičnih stanica in vitro u prisustvu lizata trombcita', Master's thesis, University of Zagreb, Faculty of Science, accessed 23 December 2024, https://urn.nsk.hr/urn:nbn:hr:217:616081

Klanfar T. Umnažanje ljudskih mezenhimskih matičnih stanica in vitro u prisustvu lizata trombcita [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2010 [cited 2024 December 23] Available at: https://urn.nsk.hr/urn:nbn:hr:217:616081

T. Klanfar, "Umnažanje ljudskih mezenhimskih matičnih stanica in vitro u prisustvu lizata trombcita", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2010. Available at: https://urn.nsk.hr/urn:nbn:hr:217:616081

Metadata
TitleUmnažanje ljudskih mezenhimskih matičnih stanica in vitro u prisustvu lizata trombcita
Title (english)Expansion of human mesenchymal stem cells in platelet lysate-containing medium
AuthorTomislav Klanfar
MentorMirna Golemović (mentor)
MentorNada Oršolić (sumentor)
Committee memberMirna Golemović (predsjednik povjerenstva)
Committee memberNada Oršolić (član povjerenstva)
Committee memberMaja Matulić (član povjerenstva)
Committee memberPerica Mustafić (član povjerenstva)
Committee memberZoran Tadić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2010, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Mezenhimske matične stanice (MMS) su nekrvotvorne multipotentne matične stanice koje su od svih njihovih tkivnih izvora najzastupljenije u koštanoj srži (KS). MMS ispoljavaju imunomodulatorna svojstva in vitro što im daje potencijalnu primjenu u staničnoj terapiji. MMS su klinički primjenjivane pri transplantacijama krvotvornih matičnih stanica i liječenju reacije pesatka-protiv-primatelja. Učestalost MMS u KS vrlo je mala te ih je za potrebe kliničke primjene neophodno umnožiti ex vivo. Mi smo umnažali MMS in vitro u kultivacijskom mediju koji je bio oplemenjen s 10% ljudskog lizata trombocita (LT) tako izbjegavajući upotrebu suplemenata životinjskog podrijetla. MMS su bile izolirane iz KS zdravih dobrovoljnih davatelja i uspješno uzgojene u mediju oplemenjenim s LT. Tijekom cijelog perioda uzgoja in vitro, stanice su ispoljavale mezenhimske karakteristike; prihvaćale su se na plastičnu podlogu, imale vretenasti fibroblastni oblik, ispoljavale imunofenotip mezenhimskih stanica i formirale fibroblastne kolonije. Ovi nalazi daju uporište za daljnja ispitivanja upotrebe LT u postupcima umnažanja MMS.
Abstract (english)
Mesenchymal stem cells (MSCs) are nonhematopoietic multipotent stem cells mainly residin in the bone marrow (BM). MSCs show immunomodulatory properties in vitro that make them intersting in the field of cellular therapy. MSCs have ben clinically applied to improve engraftment in hematopoietic stem cell transplantation and to treat acute graft versus host disease. Incidence of MSCs in BM is very low and ex vivo expansion of MSCs is necessary. We expanded MSCs in vitro in media supplemented with 10% human plateet lysate (PL) thus avoiding standard use of animal origin supplements. MSCs isolated from BM samples of healthy donors were sucessfully cultured in medium containing PL. During propagation period of 35 days, MSCs continuously expressed mesenchymal characteristics: they adhered to plastic surface shoing typical firoblastod shape, they expressed mesenchymal immunophenotype and formed fibroblast colonies. These findings give basis for further efforts in evaluating use of PL i MSCs expansion methods.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:616081
Study programmeTitle: Biology; specializations in: Molecular biology
Course: Molecular biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra biologije (magistar/magistra biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2018-03-26 07:45:28