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master's thesis
Inhibicija kolinesterazâ karbamatom albuterola
Zagreb: University of Zagreb, Faculty of Science, 2018. urn:nbn:hr:217:394151
Kerep, Robert
University of Zagreb
Faculty of Science
Department of Chemistry

Institutional repository: Repository of the Faculty of Science

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Kerep, R. (2018). Inhibicija kolinesterazâ karbamatom albuterola (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:394151

Kerep, Robert. "Inhibicija kolinesterazâ karbamatom albuterola." Master's thesis, University of Zagreb, Faculty of Science, 2018. https://urn.nsk.hr/urn:nbn:hr:217:394151

Kerep, Robert. "Inhibicija kolinesterazâ karbamatom albuterola." Master's thesis, University of Zagreb, Faculty of Science, 2018. https://urn.nsk.hr/urn:nbn:hr:217:394151

Kerep, R. (2018). 'Inhibicija kolinesterazâ karbamatom albuterola', Master's thesis, University of Zagreb, Faculty of Science, accessed 20 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:394151

Kerep R. Inhibicija kolinesterazâ karbamatom albuterola [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2018 [cited 2024 November 20] Available at: https://urn.nsk.hr/urn:nbn:hr:217:394151

R. Kerep, "Inhibicija kolinesterazâ karbamatom albuterola", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:217:394151

Metadata
TitleInhibicija kolinesterazâ karbamatom albuterola
Title (english)Inhibition of cholinesterases by albuterol carbamate
AuthorRobert Kerep
MentorAnita Bosak (mentor)
Committee memberInes Primožič (predsjednik povjerenstva)
Committee memberIva Juranović Cindrić (član povjerenstva)
Committee memberMarko Močibob (član povjerenstva)
Committee memberIvan Kodrin (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Chemistry)
Zagreb
Defense date and country2018-02-26, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
OdreĎene su konstante brzine inhibicije dviju butirilkolinesteraza (BChE; EC 3.1.1.8) i dviju acetilkolinesteraza (AChE; EC 3.1.1.7) s racemičnim, (R)- i (S)- N,N-dimetilkarbamatom albuterola (4-(2-(tert-butilamino)-1-hidroksietil)-2-(hidroksimetil)fenol. Korištene su ljudska (hBChE) i BChE izolirana iz seruma konja (hoBChE), rekombinantna ljudska AChE (hAChE) i AChE izolirana iz električnog organa jegulje (eeAChE). Karbamat albuterola je progresivno inhibirao sve ispitivane kolinesteraze s konstantama brzine inhibicije reda veličine 103-106 dm3mol-1min-1, pri čemu je najbrţe inhibirao hBChE. Ispitivani karbamat se pokazao selektivnim inhibitorom koji hBChE inhibira 8 puta brţe od hAChE. TakoĎer, karbamat albuterola razlikuje kolinesteraze različitih vrsta, budući da hBChE inhibira 1,7 puta brţe od hoBChE, dok hAChE inhibira 34 puta brţe od eeAChE. Ljudske BChE i AChE su stereoselektivni enzimi koji imaju 13, odnosno 4 puta veći afinitet prema (R)-karbamatu albuterola. Inhibicijski potencijal karbamata albuterola, kao i njegova selektivnost prema hBChE u odnosu na AChE, manja je od do sada ispitivanih karbamata slične strukture.
Abstract (english)
Inhibition rate constants of two butyrylcholinesterases (BChE, EC 3.1.1.8) and two acetylcholinesterases (AChE; EC 3.1.1.7) with racemic, (R)- and (S)- albuterol carbamate (4- (2-(tert-butylamino )-1-hydroxyethyl)-2-(hydroxymethyl)phenyl dimethylcarbamate) were determined. Human (hBChE) and BChE from the horse serum (hoBChE), recombinant human AChE (hAChE) and AChE from the eel (eeAChE) were used. Albuterol carbamate progressively inhibited all cholinesterases with inhibition rate constants ranged from 103-106 dm3mol-1min-1, wherefrom inhibition of hBChE was the fastest. Tested carbamate is a selective BChE inhibitor inhibiting hBChE 8 times faster than hAChE. Also, albuterol carbamate clearly distinguishes cholinesterases of different species, since it inhibits hBChE 1.7 times faster than hoBChE, and hAChE 34 times faster than eeAChE. Human BChE and AChE are stereoselective enzymes having 13 or 4 times higher affinity to (R)-carbamate albuterol. The inhibitory potential of albuterol carbamate, as well as its selectivity toward hBChE relative to AChE, is lower than that of other carbamates with the similar structure.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:394151
Study programmeTitle: Graduate university studies in chemistry; specializations in: Research programme
Course: Research programme
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2019-02-26
Terms of useLicence In copyright icon
Created on2018-03-27 09:41:56