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master's thesis
Regulacija aktivnosti urokinaznog sustava u ljudskim stanicama glioblastoma
Zagreb: University of Zagreb, Faculty of Science, 2011. urn:nbn:hr:217:236261
Požgaj, Lidija
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Požgaj, L. (2011). Regulacija aktivnosti urokinaznog sustava u ljudskim stanicama glioblastoma (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:236261

Požgaj, Lidija. "Regulacija aktivnosti urokinaznog sustava u ljudskim stanicama glioblastoma." Master's thesis, University of Zagreb, Faculty of Science, 2011. https://urn.nsk.hr/urn:nbn:hr:217:236261

Požgaj, Lidija. "Regulacija aktivnosti urokinaznog sustava u ljudskim stanicama glioblastoma." Master's thesis, University of Zagreb, Faculty of Science, 2011. https://urn.nsk.hr/urn:nbn:hr:217:236261

Požgaj, L. (2011). 'Regulacija aktivnosti urokinaznog sustava u ljudskim stanicama glioblastoma', Master's thesis, University of Zagreb, Faculty of Science, accessed 03 January 2025, https://urn.nsk.hr/urn:nbn:hr:217:236261

Požgaj L. Regulacija aktivnosti urokinaznog sustava u ljudskim stanicama glioblastoma [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2011 [cited 2025 January 03] Available at: https://urn.nsk.hr/urn:nbn:hr:217:236261

L. Požgaj, "Regulacija aktivnosti urokinaznog sustava u ljudskim stanicama glioblastoma", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2011. Available at: https://urn.nsk.hr/urn:nbn:hr:217:236261

Metadata
TitleRegulacija aktivnosti urokinaznog sustava u ljudskim stanicama glioblastoma
Title (english)Regulation of urokinase activation system in human glioblastoma cells
AuthorLidija Požgaj
MentorMaja Matulić (mentor)
MentorJosip Madunić (pomoć pri izradi, neposredni voditelj)
Committee memberMaja Matulić (član povjerenstva)
Committee memberMirta Tkalec (član povjerenstva)
Committee memberNada Oršolić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2011, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Sustav aktivacije plazminogena proteolitički je sustav koji ima veliku ulogu u fiziološkim i patološkim procesima u kojima dolazi do pregradnje izvanstaničnog matriksa. Sustav aktivacije plazminogena reguliran je na razini ekspresije urokinaznog i tkivnog tipa plazminogenskog aktivatora (uPA i tPA), inhibitora plazminogenskog aktivatora (PAI-1 i PAI-2) i receptora za uPA (uPAR). Osnovni je princip djelovanja sustava aktivacija plazminogena i nastanak plazmina, proteaze širokog spektra djelovanja. Cilj ovog istraživanja bio je istraživanje mehanizma regulacije sustava plazminogenske aktivacije kod stanica glioblastoma A1235, nakon alkilacijskog oštećenja, te je li u te procese uključena aktivacija MAP-kinaze JNK. Stanična linija glioblastoma A1235, kojoj nedostaje ekspresija enzima popravka O6metilguanin-metiltransferaze, nakon alkilirajućeg oštećenja inducira porast urokinazne aktivnosti. Inhibitor JNK nije pokazao znatnu inhibiciju indukcije aktivnosti uPA u stanicama A1235, nakon alkilirajućeg oštećenja. Sva ova istraživanja ukazuju na to da je regulacija urokinazne aktivnosti složen sustav, a signalni putevi indukcije ovisni o tipu stanica.
Abstract (english)
Plasminogen activation system is a proteolytic system that plays an important role in physiological and pathological processes in which extracellular matrix remodelling occurs. Plasminogen activation system is regulated on the level of transcription of urokinase-type and tissue-type plasminogen activator (uPA and tPA), plasminogen activator inhibitors (PAI-1 i PAI-2) and uPA receptor (uPAR). The aim of this study was to investigate the regulation of urokinase activation system in A1235 human glioblastoma cells after alkylation treatment and to determine whether this induction of urokinase activity was associated with activation of JNK MAP-kinase. A1235 cells, lacking the expression of the O6metilguanin methyltransferase, after alkylation damage induced increase in urokinase activity. Inhibitor JNK didn´t show significant inhibition of uPA activity in A1235 cells after alkylation damage. The experiments indicate the existance of complex pathways regulating urokinase activity, showing the cell type specificity.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:236261
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2018-04-10 09:15:23