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undergraduate thesis
Glikozilacija terapeutskih proteina
Zagreb: University of Zagreb, Faculty of Science, 2012. urn:nbn:hr:217:244346
Galić, Hrvoje
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Galić, H. (2012). Glikozilacija terapeutskih proteina (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:244346

Galić, Hrvoje. "Glikozilacija terapeutskih proteina." Undergraduate thesis, University of Zagreb, Faculty of Science, 2012. https://urn.nsk.hr/urn:nbn:hr:217:244346

Galić, Hrvoje. "Glikozilacija terapeutskih proteina." Undergraduate thesis, University of Zagreb, Faculty of Science, 2012. https://urn.nsk.hr/urn:nbn:hr:217:244346

Galić, H. (2012). 'Glikozilacija terapeutskih proteina', Undergraduate thesis, University of Zagreb, Faculty of Science, accessed 25 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:244346

Galić H. Glikozilacija terapeutskih proteina [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Science; 2012 [cited 2024 November 25] Available at: https://urn.nsk.hr/urn:nbn:hr:217:244346

H. Galić, "Glikozilacija terapeutskih proteina", Undergraduate thesis, University of Zagreb, Faculty of Science, Zagreb, 2012. Available at: https://urn.nsk.hr/urn:nbn:hr:217:244346

Metadata
TitleGlikozilacija terapeutskih proteina
Title (english)Glycosylation of therapeutic proteins
AuthorHrvoje Galić
MentorIta Gruić-Sovulj (mentor)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2012, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Mnoga impresivna dostignuća terapeutskih proteina u liječenju teških bolesti, ne bi bila moguća bez glikoinženjeringa. Terapeutski proteini su sami po sebi kratkog vijeka u organizmu i ograničene su biološke aktivnosti, stoga je nužno uvesti dodatne glikozilacijske mehanizme koji će poboljšati farmakodinamička i farmakokinetička svojstva terapeutskih proteina. U ovom radu su ukratko izloženi osnovni principi glikozilacije proteina u sve tri domene života. Poseban naglasak je stavljen na važnost samog procesa i njegovog utjecaja na stabilnost, strukturu i funkciju (terapeutskih) proteina. Detaljnije su obrađeni načini proizvodnje terapeutskih proteina, problematika glikozilacije humanih proteina u drugim organizmima, te načini postizanja optimalne glikozilacije i dobivanja proteina željene glikoforme. Navedeni su brojni primjeri poboljšanja in vivo biološke aktivnosti proteina glikoinženjeringom, od hiperglikoziliranog analoga humanog rekombinantnog eritropoetina (NESP-a) do terapeutskih protutijela i β-glukocerbrozidaze.
Abstract (english)
Many impressive achievements of therapeutic proteins in treating serious illnesses would not be possible without glycoengineering. Therapeutic proteins have a short serum half-life and limited biological activity. That is why it's necessary to include more glycosylation mechanisms which will consequentially enhance the pharmacokinetic and pharmacodynamic properties of therapeutic proteins. In this paper, the basic glycosylation principles in all domains of life are presented. A special emphasis was given to the importance of the glycosylation process itself on the stability, structure and function of (therapeutic) proteins. In more detail were explained the various methods of synthesis, the problem of glycosylation of human proteins in other organisms and various ways to obtain the wanted glycoform of a therapeutic protein. There were given many examples of in vivo enhancements of protein bioactivity via glycoengineering, from the hyperglycosylated analog of the human recombinant erythropoietin (NESP) to therapeutic antibodies and β-glucocerebrosidase.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:244346
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) molekularne biologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2018-04-16 09:34:39