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master's thesis
Ekspresija proteina djelomično neuređene tercijarne strukture u normalnim i stresnim uvjetima
Zagreb: University of Zagreb, Faculty of Science, 2014. urn:nbn:hr:217:055537
Rončević, Tomislav
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Rončević, T. (2014). Ekspresija proteina djelomično neuređene tercijarne strukture u normalnim i stresnim uvjetima (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:055537

Rončević, Tomislav. "Ekspresija proteina djelomično neuređene tercijarne strukture u normalnim i stresnim uvjetima." Master's thesis, University of Zagreb, Faculty of Science, 2014. https://urn.nsk.hr/urn:nbn:hr:217:055537

Rončević, Tomislav. "Ekspresija proteina djelomično neuređene tercijarne strukture u normalnim i stresnim uvjetima." Master's thesis, University of Zagreb, Faculty of Science, 2014. https://urn.nsk.hr/urn:nbn:hr:217:055537

Rončević, T. (2014). 'Ekspresija proteina djelomično neuređene tercijarne strukture u normalnim i stresnim uvjetima', Master's thesis, University of Zagreb, Faculty of Science, accessed 04 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:055537

Rončević T. Ekspresija proteina djelomično neuređene tercijarne strukture u normalnim i stresnim uvjetima [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2014 [cited 2024 November 04] Available at: https://urn.nsk.hr/urn:nbn:hr:217:055537

T. Rončević, "Ekspresija proteina djelomično neuređene tercijarne strukture u normalnim i stresnim uvjetima", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2014. Available at: https://urn.nsk.hr/urn:nbn:hr:217:055537

Metadata
TitleEkspresija proteina djelomično neuređene tercijarne strukture u normalnim i stresnim uvjetima
Title (english)Expression of intrinsically disordered proteins in normal and stressful conditions
AuthorTomislav Rončević
MentorDubravko Pavoković (mentor)
Committee memberDubravko Pavoković (član povjerenstva)
Committee memberDraginja Mrvoš-Sermek (član povjerenstva)
Committee memberInes Radanović (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2014, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Proteini djelomično neuređene tercijarne strukture (IDP, intrinsically disordered proteins) su uključeni u regulaciju, signalizaciju i kontrolne putove, te odgovore na razne vrste abiotičkog i biotičkog stresa. Ovo istraživanje je provedeno na N, HO i HNO linijama šećerne repe (Beta vulgaris L.). IDP su uspješno odvojeni od globularnih proteina, te su razdvojeni korištenjem modificirane poliakrilamidne gel elektroforeze. Ekspresija IDP se razlikovala između staničnih linija što ukazuje da su neki IDP regulirani razvojnim stadijem stanice. IDP iz sve tri stanične linije šećerne repe podvrgnuti su provjeri N-glikana. Rezultati upućuju da IDP nisu glikozilirani, iako to valja dodatno potvrditi. Odabrana je linija HO šećerne repe, tretirana sa 300 mM manitolom i kadmijem u tri različite koncentracije (10 μM, 40 μM i 100 μM CdCl2) kako bi izazvali vodni stres, odnosno stres teškim metalima. Tretiranje manitolom izazvalo je promjene na fenotipskoj razini, manji prirast biljnog tkiva, povećanje aktivnosti peroksidaze, te različitu ekspresiju IDP u usporedbi sa kontrolom. Tretiranje linije HO sa 100 μM kadmijem također je dovelo do odgovora na fenotipskoj razini, povećanu aktivnost peroksidaze i različitu ekspresiju IDP u usporedbi s kontrolom. Niže koncentracije kadmija nisu bile dovoljne da bi izazvale značajniji odgovor na stres izazvan teškim metalima.
Abstract (english)
Intrinsically disordered proteins (IDPs) are involved in metabolic regulation, signalization, controlling pathways and in various types of biotic and abiotic stress. This study was conducted using N, HO and HNO sugar beet (Beta vulgaris L.) lines. We have successfully separated IDPs from globular proteins, and IDPs have been further analyzed using modified polyacrylamide gel electrophoresis. IDPs expression differed between cell lines indicating some IDPs are regulated by cell developmental stage. Isolated IDPs from the cell lines were tested whether they are N–glycosylated. Results are indicating that IDPs are not glycosylated, although this remains to be verified. HO line was chosen and treated with 300 mM mannitol and cadmium in three different concentrations (10 μM, 40 μM and 100 μM CdCl 2) to cause water deficit and heavy metal stress. Mannitol treatment caused phenotype changes, slower cell growth, increase of peroxidase activity, as well as a change in IDP expression compared with control. Highest cadmium treatment also led to responses on a phenotype level, increase of peroxidase activity and different protein expression compared with control. Lower cadmium concentrations were not sufficient to produce significant response to heavy metal phytotoxicity.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:055537
Study programmeTitle: Biology and Chemistry
Study programme type: university
Study level: graduate
Academic / professional title: profesor biologije i kemije (profesor biologije i kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2018-04-26 12:38:27