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master's thesis
Povezanost koncentracije faktora rasta trombocita-AA u serumu i oštećenja jetre kod infekcije virusom hepatitisa C
Zagreb: University of Zagreb, Faculty of Science, 2018. urn:nbn:hr:217:997431
Parać, Petra
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Parać, P. (2018). Povezanost koncentracije faktora rasta trombocita-AA u serumu i oštećenja jetre kod infekcije virusom hepatitisa C (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:997431

Parać, Petra. "Povezanost koncentracije faktora rasta trombocita-AA u serumu i oštećenja jetre kod infekcije virusom hepatitisa C." Master's thesis, University of Zagreb, Faculty of Science, 2018. https://urn.nsk.hr/urn:nbn:hr:217:997431

Parać, Petra. "Povezanost koncentracije faktora rasta trombocita-AA u serumu i oštećenja jetre kod infekcije virusom hepatitisa C." Master's thesis, University of Zagreb, Faculty of Science, 2018. https://urn.nsk.hr/urn:nbn:hr:217:997431

Parać, P. (2018). 'Povezanost koncentracije faktora rasta trombocita-AA u serumu i oštećenja jetre kod infekcije virusom hepatitisa C', Master's thesis, University of Zagreb, Faculty of Science, accessed 25 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:997431

Parać P. Povezanost koncentracije faktora rasta trombocita-AA u serumu i oštećenja jetre kod infekcije virusom hepatitisa C [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2018 [cited 2024 November 25] Available at: https://urn.nsk.hr/urn:nbn:hr:217:997431

P. Parać, "Povezanost koncentracije faktora rasta trombocita-AA u serumu i oštećenja jetre kod infekcije virusom hepatitisa C", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:217:997431

Metadata
TitlePovezanost koncentracije faktora rasta trombocita-AA u serumu i oštećenja jetre kod infekcije virusom hepatitisa C
Title (english)Correlation between platelet derived growth factor-AA concentration in serum and liver damage in hepatitis C virus infection
AuthorPetra Parać
MentorSnježana Židovec Lepej (mentor)
Committee memberSnježana Židovec Lepej (član povjerenstva)
Committee memberSilvija Černi (član povjerenstva)
Committee memberDomagoj Đikić (član povjerenstva)
Committee memberPetar Kružić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2018-03-09, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Virus hepatitisa C (HCV) je RNA-virus iz porodice Flaviviridae i jedan je od vodećih uzroka pobola i smrti u svijetu. Kronična infekcija virusom HCV povezana je s nastankom fibroze jetre, ciroze te hepatocelularnog karcinoma. Faktori rasta trombocita (PDGF) reguliraju proliferaciju, migraciju, preživljavanje, kemotaksiju stanica i angiogenezu. Homodimer faktora rasta PDGF-AA važan je regulator uloge fibroblasta u zacjeljivanju rana. Cilj ovog istraživanja bio je odrediti koncentraciju faktora rasta PDGF-AA u serumu osoba zaraženih virusom HCV i zdravih kontrola te usporediti koncentraciju ovog faktora rasta u osoba s različitim stupnjevima tvrdoće jetre (LMS) i fibroze. Analizirana je povezanost između koncentracije faktora rasta PDGF-AA i stupnja fibroze, koncentracije jetrenih enzima, lipida i proteina u serumu te kinetike virusne replikacije. Za kvantifikaciju faktora rasta PDGF-AA korišten je enzimski imunotest, a za kvantifikaciju RNA virusa HCV korištena je kvantitativna lančana reakcija polimerazom u stvarnom vremenu. Rezultati ovog istraživanja pokazali su statistički značajnu razliku u koncentraciji faktora rasta PDGF-AA u zdravih osoba i osoba s infekcijom virusom HCV te značajnu korelaciju između koncentracije faktora rasta PDGF-AA i triglicerida, albumina te kolesterola LDL. Dokazana je pozitivna korelacija između LMS i vrijednosti enzima jetre AST, ALT, GGT i bilirubina, a negativna korelacija između LMS i koncentracije kolesterola, albumina i kolesterola LDL. Rezultati ovog istraživanja ukazuju na moguću važnost faktora rasta PDGF-AA u patogenezi kroničnog hepatitisa C.
Abstract (english)
Hepatitis C virus (HCV) is an RNA-virus belonging to Flaviviridae family and is one of the leading causes of death and morbidity in the world. Chronic hepatitis C infection is related to the occurence of liver fibrosis, cirrhosis and hepatocellular carcinoma. Platelet derived growth factors (PDGF) regulate cell proliferation, migration, survival, chemotaxis and angiogenesis. Homodimer PDGF-AA is an important regulator of fibroblasts during wound healing. The aim of this study was to determine concentrations of PDGF-AA in serum of patients with HCV infection and healthy controls and to compare concentrations of PDGF-AA with the difference in levels of liver stiffness (LMS) and fibrosis. Connection between PDGF-AA concentration and fibrosis stage, concentration of liver enzymes, lipids and proteins in the serum as well as viral replication kinetics has been analyzed. Enzyme immunotest was used for the quantification of PDGF-AA while quantitative real time polymerase chain reaction was used to quantify HCV RNA. The results of this research showed a statistically significant difference in the concentration of PDGF-AA in healthy people and people infected with HCV and significant correlation between the concentration of PDGF-AA and triglycerides, albumins and LDLs. Positive correlation was found between LMS and serum levels of AST, ALT, GGT and bilirubin and negative correlation was found between LMS and serum levels of cholesterol, albumin and LDL. The resuts of this research show a possible part PDGF-AA might play in the pathogenesis of chronic hepatitis C.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:997431
Study programmeTitle: Experimental Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2018-05-04 11:38:07