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master's thesis
Tumor supresorski učinak Sirt3 i hiperoksije na stanice tumora dojke MCF-7
Zagreb: University of Zagreb, Faculty of Science, 2018. urn:nbn:hr:217:459040
Davidović, Grazia
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Davidović, G. (2018). Tumor supresorski učinak Sirt3 i hiperoksije na stanice tumora dojke MCF-7 (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:459040

Davidović, Grazia. "Tumor supresorski učinak Sirt3 i hiperoksije na stanice tumora dojke MCF-7." Master's thesis, University of Zagreb, Faculty of Science, 2018. https://urn.nsk.hr/urn:nbn:hr:217:459040

Davidović, Grazia. "Tumor supresorski učinak Sirt3 i hiperoksije na stanice tumora dojke MCF-7." Master's thesis, University of Zagreb, Faculty of Science, 2018. https://urn.nsk.hr/urn:nbn:hr:217:459040

Davidović, G. (2018). 'Tumor supresorski učinak Sirt3 i hiperoksije na stanice tumora dojke MCF-7', Master's thesis, University of Zagreb, Faculty of Science, accessed 23 December 2024, https://urn.nsk.hr/urn:nbn:hr:217:459040

Davidović G. Tumor supresorski učinak Sirt3 i hiperoksije na stanice tumora dojke MCF-7 [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2018 [cited 2024 December 23] Available at: https://urn.nsk.hr/urn:nbn:hr:217:459040

G. Davidović, "Tumor supresorski učinak Sirt3 i hiperoksije na stanice tumora dojke MCF-7", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:217:459040

Metadata
TitleTumor supresorski učinak Sirt3 i hiperoksije na stanice tumora dojke MCF-7
Title (english)Tumor suppressive effect of Sirt3 and hyperoxia on MCF-7 breast cancer cells
AuthorGrazia Davidović
MentorSandra Sobočanec (mentor)
MentorDomagoj Đikić (mentor)
Committee memberDomagoj Đikić (član povjerenstva)
Committee memberTomislav Ivanković (član povjerenstva)
Committee memberŽeljka Vidaković-Cifrek (član povjerenstva)
Committee memberInga Marijanović (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2018-04-18, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Oksidacijski stres i visoke razine reaktivnih kisikovih radikala izazivaju oštećenja DNA i genomsku nestabilnost, koje dovode do promjene staničnog metabolizma i do gubitka kontrole staničnog ciklusa. Takvi uvjeti podržavaju stalni rast i proliferaciju, glavno obilježje tumorskih stanica. Iako se dugo raspravljalo o djelovanju čistog kisika (hiperoksije) kao promotoru malignih bolesti, danas se hiperoksija koristi kao rutinska terapija u liječenju upravo takvih oboljenja. Sirtuin 3 (Sirt3) je glavna mitohondrijska NAD+ - ovisna deacetilaza koja učinkovito sudjeluje u održavanju energetske homeostaze u stanicama. U ovom radu pokazan je tumor supresorski učinak Sirt3 u kombinaciji s hiperoksijom na humanim stanicama MCF-7. Pokazano je da de novo ekspresija Sirt3 u uvjetima normoksije uzrokuje tumor-supresivni fenotip smanjujući metaboličku aktivnost, stanični rast i proliferaciju tumorskih stanica. To je dodatno posredovano smanjenom ekspresijom pro-angiogenih gena i gena uključenih u epitelno mezenhimalnu tranziciju, povećanjem mitohondrijske funkcije, te smanjenjem broja tumorskih stanica i njihovim potencijalom za stvaranje kolonija. Dok tretman hiperoksijom samostalno inhibira rast tumorskih stanica, učinak Sirt3 kao tumor supresora u tim je uvjetima još izraženiji. Kombinirani učinak Sirt3 i hiperoksije na tumorske stanice MCF-7 ukazuje da bi njihov sinergistički učinak na lokalizaciju tumora i smanjivanje metastatskog potencijala trebalo dodatno istražiti kao potencijalnu terapiju za liječenje malignih oboljenja.
Abstract (english)
Oxidative stress and high levels of reactive oxygen species cause DNA damage and genomic instability, thus leading to changes in cell metabolism and loss of the cell cycle control. These conditions support continuous growth and proliferation, the main characteristics of tumor cells. Although the role of pure oxygen (hyperoxia) as a promoter of malignant diseases has long been debated, today hyperoxia is used as a routine therapy to treat such diseases. Sirtuin 3 (Sirt3) is the major mitochondrial NAD+-dependent deacetylase which keeps the cell in energetic homeostasis. This thesis presents tumor suppressing effect of Sirt3 combined with hyperoxia in human MCF-7 cells. De novo expressed Sirt3 in normoxia causes a tumor-suppressive phenotype that reduces metabolic activity, growth and proliferation of tumor cells, expression of pro-angiogenic genes and genes involved in epithelial mesenchymal transition, increases mitochondrial function, decreases number of tumor cells and potential for colony creation. Whilst hyperoxia alone inhibits the growth of tumor cells, Sirt3 exhibits even more pronounced tumor suppressing effects under these conditions. The combined effect of Sirt3 and hyperoxia on MCF-7 cells suggests that their synergistic effect on tumor localization and reduction of metastatic potential should be further explored as a potential therapy for malignant diseases.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:459040
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2018-05-16 08:41:51