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doctoral thesis
Učinak pentadekapeptida BPC 157 na agregaciju trombocita i viskoelastična svojstva krvnog ugruška u štakora
Zagreb: University of Zagreb, Faculty of Science, 2017. urn:nbn:hr:217:304258
Konosić, Sanja
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Konosić, S. (2017). Učinak pentadekapeptida BPC 157 na agregaciju trombocita i viskoelastična svojstva krvnog ugruška u štakora (Doctoral thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:304258

Konosić, Sanja. "Učinak pentadekapeptida BPC 157 na agregaciju trombocita i viskoelastična svojstva krvnog ugruška u štakora." Doctoral thesis, University of Zagreb, Faculty of Science, 2017. https://urn.nsk.hr/urn:nbn:hr:217:304258

Konosić, Sanja. "Učinak pentadekapeptida BPC 157 na agregaciju trombocita i viskoelastična svojstva krvnog ugruška u štakora." Doctoral thesis, University of Zagreb, Faculty of Science, 2017. https://urn.nsk.hr/urn:nbn:hr:217:304258

Konosić, S. (2017). 'Učinak pentadekapeptida BPC 157 na agregaciju trombocita i viskoelastična svojstva krvnog ugruška u štakora', Doctoral thesis, University of Zagreb, Faculty of Science, accessed 06 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:304258

Konosić S. Učinak pentadekapeptida BPC 157 na agregaciju trombocita i viskoelastična svojstva krvnog ugruška u štakora [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Science; 2017 [cited 2024 November 06] Available at: https://urn.nsk.hr/urn:nbn:hr:217:304258

S. Konosić, "Učinak pentadekapeptida BPC 157 na agregaciju trombocita i viskoelastična svojstva krvnog ugruška u štakora", Doctoral thesis, University of Zagreb, Faculty of Science, Zagreb, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:217:304258

Metadata
TitleUčinak pentadekapeptida BPC 157 na agregaciju trombocita i viskoelastična svojstva krvnog ugruška u štakora
Title (english)Effect of pentadecapeptide BPC 157 on platelet aggregation and viscoelastic properties of blood clot in rats
AuthorSanja Konosić
MentorPredrag Sikirić (mentor)
MBZ: 117454
Committee memberAleksandar Vojta (predsjednik povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2017, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Universal decimal classification
(UDC)		577 - Biochemistry. Molecular biology. Biophysics
Abstract
Prethodna istraživanja pokazala su da BPC 157 sprječava nastanak okluzivnog tromba i ubrzava razgradnju već stvorenog ugruška nakon formiranja anastomoze aorte, a da, s druge strane, smanjuje vrijeme krvarenja kod štakora tretiranih varfarinom, heparinom i aspirinom. Temeljem toga, nametnula se potreba određivanja njegova utjecaja na agregaciju trombocita i viskoelastična svojstva krvnog ugruška. Za ispitivanje eventualne uključenosti NO sustava u djelovanje BPC 157 korišten je selektivni inhibitor topljive gvanilil ciklaze, ODQ. Životinje (n=60) su najprije podijeljene u skupine ovisno o tome koji antiagregacijski lijek primaju (1.aspirin, 2.klopidogrel, 3.cilostazol), a te skupine su tada nasumično podijeljene u četiri podskupine koje su primale a) fiziološku otopinu, b) BPC 157, c) ODQ, d) BPC 157 + ODQ. Mjerenja su izvršena impendancijskom agregometrijom s 4 različita agonista (ADP, AA, AA/PGE1, kolagen), te rotacijskom tromboelastometrijom s 3 različita agonista (preko vanjskog i unutarnjeg puta zgrušavanja, te bez doprinosa trombocita). Iz rezultata dobivenih usporedbom podskupina a i b, te b i d unutar svake skupine možemo zaključiti da BPC 157 oporavlja inhibiranu agregaciju trombocita, ali da nema utjecaja na viskoelastična svojstva krvnog ugruška u ispitivanih štakora.
Abstract (english)
As a natural extension of previous research that confirmed the role of BPC 157 in the prevention of obstructive thrombus formation and rapid destruction of an already formed one after aortic anastomosis, but also shortening of the bleeding time in rats treated with anticoagulants and aspirin, there was a necessity to determine how BPC 157 influences platelet aggregation and viscoelastic properties of the blood clot. To assess its relation to NO system, sCG selective inhibitor (ODQ) was used. Rats (n=60) were divided into groups depending on the antiaggregatory drug they were treated with (1.aspirin, 2.clopidogrel, 3.cilostazol). Groups were further divided into four subgroups treated with a) normal saline, b) BPC 157, c) ODQ, d) BPC 157 + ODQ. Impendance aggregomery measurements with four agonists (ADP, AA, AA/PGE1 and collagen), and also rotational thromboelastometric measurements with 3 agonists (for initiating external and internal coagulation pathway and without platelet contribution) were performed. Based on the results obtained by comparing subgroups a versus b, and b versus d in each group, we can conclude that BPC 157 rescues inhibited platelet aggregation, but it has no effect on viscoelastic properties of the blood clot.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:304258
Study programmeTitle: Biology
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija (doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-03-20 11:44:50