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master's thesis
Uloga šaperona Hsp42 u metabolizmu kvasca Saccharomyces cerevisiae
Zagreb: University of Zagreb, Faculty of Science, 2016. urn:nbn:hr:217:376368
Kurtović, Matea
University of Zagreb
Faculty of Science
Department of Chemistry

Institutional repository: Repository of the Faculty of Science

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Kurtović, M. (2016). Uloga šaperona Hsp42 u metabolizmu kvasca Saccharomyces cerevisiae (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:376368

Kurtović, Matea. "Uloga šaperona Hsp42 u metabolizmu kvasca Saccharomyces cerevisiae." Master's thesis, University of Zagreb, Faculty of Science, 2016. https://urn.nsk.hr/urn:nbn:hr:217:376368

Kurtović, Matea. "Uloga šaperona Hsp42 u metabolizmu kvasca Saccharomyces cerevisiae." Master's thesis, University of Zagreb, Faculty of Science, 2016. https://urn.nsk.hr/urn:nbn:hr:217:376368

Kurtović, M. (2016). 'Uloga šaperona Hsp42 u metabolizmu kvasca Saccharomyces cerevisiae', Master's thesis, University of Zagreb, Faculty of Science, accessed 21 December 2024, https://urn.nsk.hr/urn:nbn:hr:217:376368

Kurtović M. Uloga šaperona Hsp42 u metabolizmu kvasca Saccharomyces cerevisiae [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2016 [cited 2024 December 21] Available at: https://urn.nsk.hr/urn:nbn:hr:217:376368

M. Kurtović, "Uloga šaperona Hsp42 u metabolizmu kvasca Saccharomyces cerevisiae", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2016. Available at: https://urn.nsk.hr/urn:nbn:hr:217:376368

Metadata
TitleUloga šaperona Hsp42 u metabolizmu kvasca Saccharomyces cerevisiae
Title (english)The role of molecular chaperone Hsp42 in metabolism regulation of Saccharomyces cerevisiae
AuthorMatea Kurtović
MentorIta Gruić Sovulj (mentor)
Committee memberIta Gruić Sovulj (predsjednik povjerenstva)
Committee memberIva Juranović Cindrić (član povjerenstva)
Committee memberIvica Đilović (član povjerenstva)
Committee memberJasmina Rokov Plavec (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Chemistry)
Zagreb
Defense date and country2016-10-10, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
Stanica aktivno održava uravnotežen odnos između sinteze proteina, održavanja njihove konformacije i degradacije oštećenih proteina. Molekularni šaperoni su proteini čija je važna uloga održavanje homeostaze proteina (proteostaze). U ovom radu konstruirana su dva nova kvaščeva soja,hsp42snf1 i hsp42zwf1. U oba soja deletiran je gen za šaperon Hsp42 te su deletirani ciljani geni koji kodiraju proteine metabolizma glukoze; kinaza Snf1 i glukoza-6-fosfat-dehidrogenaza (zwf1). Za dobivene sojeve mjerena je količina staničnog ATP-a, stupanj karbonilacije proteina, razina reaktivnih kisikovih vrsta, frekvencija mutacija i kronološki životni vijek. Rezultati pokazuju da umjereni stres poput delecije jednog šaperona produžuje kronološki životni vijek i inducira toleranciju na stres, dok je u soju hsp42snf1 kronološki životni vijek skraćen. Iako su novokontruirani mutanti izloženi proteotoksičnom stresu takav oblik stresa nije u korelaciji s proizvodnjom ATP-a u sojevima hsp42zwf1 i hsp42snf1. Pokazano je i da je u novokonstruiranim sojevima značajno povećana frekvencija mutacija.
Abstract (english)
The cell actively maintains a balanced relation between protein synthesis, preservation of their stability and conformation, and degradation of damaged proteins. Molecular chaperons are proteins with the important role of maintaining protein homeostasis (proteostasis). In this thesis two yeast strais were newly constructed, hsp42snf1 and hsp42zwf1.In both strains a gene for molecular chaperone Hsp42 was deleted, along with the target genes which encode for proteins that participate in glucose metabolism; Snf1 kinase and glucose-6-phosphate dehidrogenase (zwf1). Measurments of the ATP levels, protein carbonylation levels, ROS levels, frequency of mutation and chronological lifespan were performed. The results demonstrate that a mild stress like the chaperone deletion extends the chronological lifespan and induces stress tolerability, while the chronological lifespan is shortened in the strain hsp42snf1.It is also shown that frequency of mutation in the above mentioned strains is highly increased.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:376368
Study programmeTitle: Graduate Studies in Chemistry; specializations in: Research (scientific-professional) programme
Course: Research (scientific-professional) programme
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-01-26 10:50:46