Research of the native flora in the settlement Bregana in the northwest Croatia, Zagreb County, was carried out during the year 2017. In the area of 2.8 km², for which so far there is no information about the flora, a total of 232 taxa of vascular plants from 178 genera and 75 families were found. Of the identified taxa, most belonged to Asteraceae (9.8%), Lamiaceae (7.5%), Fabaceae (7,5%), Ranunculaceae (5,1%), Rosaceae (4.6%), Poaceae (4,6%) and Cichoriaceae (4,2%). Regarding the life form spectrum hemicryptophytes (53%) were dominant, followed by phanerophytes and geophytes (15,1%), and therophytes (11,6%). Phytogeographical analysis showed that investigated area belonged to the Euro-Siberian-North American region. According to IUCN Red list, one of recorded taxa belongs to EN category, one to VU category, six to NT category and two belong to LC category. Helleborus atrorubens Waldst. et Kit is the only recorded endemic species. Also, 29 allochthonous plants were recorded from which ten were invasive alien species.