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doctoral thesis
Pročišćavanje i svojstva virusa zaušnjaka i ospica
Zagreb: University of Zagreb, Faculty of Science, 2018. urn:nbn:hr:217:593988
Sviben, Dora
University of Zagreb
Faculty of Science
Department of Chemistry

Institutional repository: Repository of the Faculty of Science

Cite this document

Sviben, D. (2018). Pročišćavanje i svojstva virusa zaušnjaka i ospica (Doctoral thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:593988

Sviben, Dora. "Pročišćavanje i svojstva virusa zaušnjaka i ospica." Doctoral thesis, University of Zagreb, Faculty of Science, 2018. https://urn.nsk.hr/urn:nbn:hr:217:593988

Sviben, Dora. "Pročišćavanje i svojstva virusa zaušnjaka i ospica." Doctoral thesis, University of Zagreb, Faculty of Science, 2018. https://urn.nsk.hr/urn:nbn:hr:217:593988

Sviben, D. (2018). 'Pročišćavanje i svojstva virusa zaušnjaka i ospica', Doctoral thesis, University of Zagreb, Faculty of Science, accessed 09 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:593988

Sviben D. Pročišćavanje i svojstva virusa zaušnjaka i ospica [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Science; 2018 [cited 2024 November 09] Available at: https://urn.nsk.hr/urn:nbn:hr:217:593988

D. Sviben, "Pročišćavanje i svojstva virusa zaušnjaka i ospica", Doctoral thesis, University of Zagreb, Faculty of Science, Zagreb, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:217:593988

Metadata
TitlePročišćavanje i svojstva virusa zaušnjaka i ospica
Title (english)Purification and properties of mumps and measles virus
AuthorDora Sviben
MentorBeata Halassy (mentor)
MentorMarija Brgles (komentor)
Committee memberMarko Močibob (predsjednik povjerenstva)
Committee memberBranimir Bertoša (član povjerenstva)
Committee memberAndreja Ambriović Ristov (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Chemistry)
Zagreb
Defense date and country2018-06-27, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Universal decimal classification
(UDC)		54 - Chemistry. Crystallography. Mineralogy
Abstract
Virusi zaušnjaka (MuV) i ospica (MeV) uzrokuju istoimene bolesti u ljudi koje se mogu spriječiti cijepljenjem, a dobivaju na značaju i u onkolitičkoj viroterapiji. Cjelovitost i čistoća virusnih čestica osiguravaju učinkovitost i sigurnost ovakvih proizvoda, stoga je njihovo pročišćavanje i koncentriranje izuzetno važno u biotehnologiji, ali i temeljnim istraživanjima. Ciljevi ovog rada su ispitati pročišćavanje MuV-a i MeV-a kromatografijom hidrofobnih interakcija (HIC) i ionske izmjene (IEX), imunoafinitetnom kromatografijom (IAC), ultracentrifugiranjem (UC) i dijafiltracijom (DF), ispitati stabilnost virusa u uvjetima pročišćavanja i čuvanja te istražiti njihove lipidome i proteome. Rezultati pokazuju da IEX nije prikladan za pročišćavanje MuV-a i MeV-a, dok HIC i IAC daju relativno visoke prinose infektivnih virusa (60-70%). Na kromatografske prinose značajno utječe promjenjiva heterogenost početnih virusnih uzoraka. Analiza proteoma virusa potvrdila je prisutnost svih strukturnih virusnih proteina te staničnih proteina aktina, aneksina, ciklofilina A, integrina β1 i moezina, a njihov značaj u virionima tek treba utvrditi.
Abstract (english)
Mumps (MuV) and measles (MeV) virus cause mumps and measles in humans, both preventable by vaccination, but they are gaining importance in oncolytic virotherapy. Viral particle integrity and purity ensure efficacy and safety of such products, therefore their purification and concentration is exceptionally important both in biotechnology and basic research. The aims of this work are to investigate purification of MuV and MeV by hydrophobic interaction (HIC), ion-exchange (IEX) and immunoaffinity chromatography (IAC), ultracentrifugation (UC) and diafiltration (DF), investigate virus stability under used purification and storage conditions and investigate their lipidomes and proteomes. Results deem IEX unsuitable for MuV and MeV purification, while HIC and IAC yield relatively high recoveries of infective viruses (60-70%). Chromatographic yields are significantly influenced by initial virus sample heterogeneity. Analysis of virus proteomes confirmed the presence of all structural viral proteins and cellular proteins actin, annexins, cyclophilin A, integrin β1 and moesin, but their significance in virions remains to be determined.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:593988
Study programmeTitle: Doctoral study in chemistry
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje kemija (doktor/doktorica znanosti, područje prirodnih znanosti, polje kemija)
Type of resourceText
Extent226 str. ; 30 cm
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2018-07-10 09:52:13