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undergraduate thesis
REPAIR - novi alat za mijenjanje genoma pomoću Cas13
Zagreb: University of Zagreb, Faculty of Science, 2018. urn:nbn:hr:217:431661
Majsec, Petra
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Majsec, P. (2018). REPAIR - novi alat za mijenjanje genoma pomoću Cas13 (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:431661

Majsec, Petra. "REPAIR - novi alat za mijenjanje genoma pomoću Cas13." Undergraduate thesis, University of Zagreb, Faculty of Science, 2018. https://urn.nsk.hr/urn:nbn:hr:217:431661

Majsec, Petra. "REPAIR - novi alat za mijenjanje genoma pomoću Cas13." Undergraduate thesis, University of Zagreb, Faculty of Science, 2018. https://urn.nsk.hr/urn:nbn:hr:217:431661

Majsec, P. (2018). 'REPAIR - novi alat za mijenjanje genoma pomoću Cas13', Undergraduate thesis, University of Zagreb, Faculty of Science, accessed 25 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:431661

Majsec P. REPAIR - novi alat za mijenjanje genoma pomoću Cas13 [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Science; 2018 [cited 2024 November 25] Available at: https://urn.nsk.hr/urn:nbn:hr:217:431661

P. Majsec, "REPAIR - novi alat za mijenjanje genoma pomoću Cas13", Undergraduate thesis, University of Zagreb, Faculty of Science, Zagreb, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:217:431661

Metadata
TitleREPAIR - novi alat za mijenjanje genoma pomoću Cas13
Title (english)REPAIR - new tool for RNA editing with Cas13
AuthorPetra Majsec
MentorIvana Ivančić Baće (mentor)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2018, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Istraživači su nedavno razvili alat REPAIR koji se sastoji od spojene katalitičke domene deaminaze ADAR i katalitički mrtvog Cas13b, iz obitelji proteina Cas13. Tako napravljen enzim ima mogućnost specifičnog uređivanja molekule RNA od interesa u ljudskim stanicama. Enzim ADAR, adenozin deaminaza djelujuća na RNA, pretvara adenozin u inozin - nukleotid koji je funkcionalni ekvivalent gvanozinu. Alat je uspješno korišten za revertiranje dviju mutacija G-u-A povezanih nastankom bolesti na mRNA – jedna mutacija uzrokuje X-vezani nefrogeni dijabetes, a druga uzrokuje Fanconi anemiju.
Abstract (english)
Researchers have created a fusion protein between the catalytic domain of deaminase ADAR and a catalytically inert Cas13b, member of Cas13 protein family, with the ability to target any RNA of interest in human cells creating REPAIR system. Enzyme ADAR, which is adenosine deaminases acting on RNA converts adenosine into inosine - a nucleotide that is functionally equivalent to guanosin. They used it to revert two disease-associated G-to-A mutations, one that causes a form of diabetes, and another that causes Fanconi anemia, in two separate messenger RNAs.
Keywords
Languagecroatian
URN:NBNurn:nbn:hr:217:431661
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) molekularne biologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2018-10-10 09:16:27