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undergraduate thesis
Uloga transpozona u genomskom doziranju kod vrste Arabidopsis thaliana
Zagreb: University of Zagreb, Faculty of Science, 2018. urn:nbn:hr:217:774849
Ručević, Leda
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Ručević, L. (2018). Uloga transpozona u genomskom doziranju kod vrste Arabidopsis thaliana (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:774849

Ručević, Leda. "Uloga transpozona u genomskom doziranju kod vrste Arabidopsis thaliana." Undergraduate thesis, University of Zagreb, Faculty of Science, 2018. https://urn.nsk.hr/urn:nbn:hr:217:774849

Ručević, Leda. "Uloga transpozona u genomskom doziranju kod vrste Arabidopsis thaliana." Undergraduate thesis, University of Zagreb, Faculty of Science, 2018. https://urn.nsk.hr/urn:nbn:hr:217:774849

Ručević, L. (2018). 'Uloga transpozona u genomskom doziranju kod vrste Arabidopsis thaliana', Undergraduate thesis, University of Zagreb, Faculty of Science, accessed 17 May 2024, https://urn.nsk.hr/urn:nbn:hr:217:774849

Ručević L. Uloga transpozona u genomskom doziranju kod vrste Arabidopsis thaliana [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Science; 2018 [cited 2024 May 17] Available at: https://urn.nsk.hr/urn:nbn:hr:217:774849

L. Ručević, "Uloga transpozona u genomskom doziranju kod vrste Arabidopsis thaliana", Undergraduate thesis, University of Zagreb, Faculty of Science, Zagreb, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:217:774849

Metadata
TitleUloga transpozona u genomskom doziranju kod vrste Arabidopsis thaliana
Title (english)Role of transposons in genome dosage response in Arabidopsis thaliana
AuthorLeda Ručević
MentorIvana Ivančić Baće (mentor)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2018, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Nizom istraživanja na Arabidopsis thaliana i drugim biljnim modelnim organizmima utvrđeno je da se s očinske strane triploidna barijera uspostavlja nakupljanjem easiRNA, čija je relativna količina ovisna o genomskoj dozi. U tome važnu ulogu igraju miRNA, to jest specifično miR845a i miR845b, koje su visoko očuvane u većini biljnih porodica, koje stupaju u interakciju s mjestom vezanja početnice LTR retrotranspozona te služe kao okidač za sintezu easiRNA. Činjenica da prilikom križanja jedinki divljeg tipa i nereduciranih mutanata postoji mala količina vijabilnog sjemena ukazuje na to da postoje prirodne varijacije u količini miR845 u populaciji koje omogućuju da događaji poliploidizacije u prirodi dovedu do specijacije i stvaranja novih biljnih vrsta. Isto tako, činjenica da postotak smanjenja aktivnosti miR845 ne korelira direktno s postotkom vijabilnog sjemena ukazuje na postojanje drugih, još neistraženih puteva uspostavljanja triploidne barijere te da samo djelovanje miR845 nije dovoljno kako bi se uspostavila triploidna barijera.
Abstract (english)
For the longest time, the mechanisms preventing the crossbreeding of certain plant species eluded us. However, a wealth of research conducted in the recent years has shed substantial light on the topic. It has been concluded that the triploid block on the paternal side is established via a kind of counting of chromosomes. An important role in this counting is played by miRNA (specifically miR845a and miR845b in Arabidopsis thaliana), which triggers the dose-dependent acummulation of easiRNA (epigenetically-activated small interfering RNA). The counting process is conducted in the endosperm of the seed. Owing to the way it is formed, the ratio of maternal to paternal genetic material in the endosperm should be 2:1. If this ratio is disturbed (e.g. an excess of paternal genetic material is detected), the seed aborts. However, these easiRNA play only a small part in establishing the triploid block. This is demonstrated in part by the small amount of wild type seeds that remain viable despite the triploid block being in place. What’s more, a large part of seeds remain non-viable, even when the activity of miR845 is downregulated. This could in part be due to the role of maternal small RNAs, whose part in establishing the triploid block is still poorly understood.
Keywords
Languagecroatian
URN:NBNurn:nbn:hr:217:774849
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) molekularne biologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2018-10-15 12:23:56