Temporary rivers and streams are dynamic ecosystems in which water stops flowing periodically and flows become terrestrial habitats that can contain isolated pools and lakes. This research was conducted along a temporary karst river Krčić, situated beneath the Dinara Mountain. Krčić is a small river which usually dries up during summer and early autumn months. It is minimally influenced by anthropogenic activities and can thus be regarded as a reference site of temporary rivers in this region. The sampling was performed in 2016 during the flowing phase; in June and December, on the upper, middle and lower reaches. The sampling encompassed all major microhabitat types (moss, fital, megalital, makrolital, mikrolital and xylal). On each site the physico-chemical parameters were measured and pH value and conductivity had the highest influence on the community. Community composition and structure recorded were typical for temporary rivers, with Chironomidae as the most abundant insect group (14 taxa), followed by Plecoptera (4 taxa), Ephemeroptera and Trichoptera (3 taxa). Generally, high diversity and evenness of the community was observed. The results of Cluster analyses and MDS showed macroinvertebrate assemblage separation by sampling seasons, not by macrohabitats. These results highlight the importance of seasonality in temporary rivers and streams, why it is necessary to perform seasonal monitoring at least twice a year.