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master's thesis
Ekspanzijska mikroskopija u proučavanju arhitekture diobenog vretena
Zagreb: University of Zagreb, Faculty of Science, 2018. urn:nbn:hr:217:502098
Ponjavić, Ivana
University of Zagreb
Faculty of Science
Department of Biology
University of Zagreb
Faculty of Science
Department of Chemistry

Institutional repository: Repository of the Faculty of Science

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Ponjavić, I. (2018). Ekspanzijska mikroskopija u proučavanju arhitekture diobenog vretena (Master's thesis). Zagreb: University of Zagreb, Faculty of Science; Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:502098

Ponjavić, Ivana. "Ekspanzijska mikroskopija u proučavanju arhitekture diobenog vretena." Master's thesis, University of Zagreb, Faculty of Science; University of Zagreb, Faculty of Science, 2018. https://urn.nsk.hr/urn:nbn:hr:217:502098

Ponjavić, Ivana. "Ekspanzijska mikroskopija u proučavanju arhitekture diobenog vretena." Master's thesis, University of Zagreb, Faculty of Science; University of Zagreb, Faculty of Science, 2018. https://urn.nsk.hr/urn:nbn:hr:217:502098

Ponjavić, I. (2018). 'Ekspanzijska mikroskopija u proučavanju arhitekture diobenog vretena', Master's thesis, University of Zagreb, Faculty of Science; University of Zagreb, Faculty of Science, accessed 29 December 2024, https://urn.nsk.hr/urn:nbn:hr:217:502098

Ponjavić I. Ekspanzijska mikroskopija u proučavanju arhitekture diobenog vretena [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; Zagreb: University of Zagreb, Faculty of Science; 2018 [cited 2024 December 29] Available at: https://urn.nsk.hr/urn:nbn:hr:217:502098

I. Ponjavić, "Ekspanzijska mikroskopija u proučavanju arhitekture diobenog vretena", Master's thesis, University of Zagreb, Faculty of Science, Zagreb; University of Zagreb, Faculty of Science, Zagreb, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:217:502098

Metadata
TitleEkspanzijska mikroskopija u proučavanju arhitekture diobenog vretena
Title (english)Expansion microscopy in exploring the architecture of the mitotic spindle
AuthorIvana Ponjavić
MentorIva Tolić (mentor)
MentorMirjana Pavlica (mentor)
Committee memberMirjana Pavlica (predsjednik povjerenstva)
Committee memberInes Radanović (član povjerenstva)
Committee memberJasmina Rokov Plavec (član povjerenstva)
Committee memberBranimir Bertoša (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
GranterUniversity of Zagreb
Faculty of Science
(Department of Chemistry)
Zagreb
Defense date and country2018-10-01, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Ekspanzijska mikroskopija je nova metoda mikroskopije u kojoj se kemijskim postupcima fizički povećava fiksirani uzorak te dozvoljava da strukture bliže od granice difrakcije svjetlosti (250 nm) postanu razlučive. To je tehnika u kojoj su fluorofori na fiksiranom uzorku vezani za polimer koji ima mogućnost izotropnog ekspandiranja te kada se polimer ekspandira, omogućava super-rezolucijsku mikroskopiju sa standardnim mikroskopom. Cilj ovog rada bio je uz pomoć razvijene metode ekspanzijske mikroskopije, proučiti arhitekturu diobenog vretena, molekularne strukture odgovorne za jednaku raspodjelu kromosoma među stanicama kćerima. Vizualizirane i analizirane su RPE1 i U2OS stanice. U ovom radu razvijen je protokol za ekspanzijsku mikroskopiju, te je dobivena slika cjelovitog diobenog vretena u ljudskim stanicama uz upotrebu ekspanzijske mikroskopije. Pokazano je da snimke ekspandiranih diobenih vretena nude mnoštvo informacija o arhitekturi vretena te je u budućnosti potrebno optimizirati protokol za bilo koji protein, koji se želi vizualizirati u diobenom vretenu.
Abstract (english)
Expansion microscopy is a newly discovered microscopy technique that uses physical expansion of fixed specimens to allow features closer than the diffraction limit of light (250 nm) to become resolvable in the expanded specimen. It is a technique in which fluorophores on fixed specimens are linked to a swellable polymer that is physically expanded to enable super-resolution microscopy with ordinary microscopes. The goal of this thesis was, with the help od the developed expansion microscopy technique, to study the architecture od the mitotic spindle, a molecular machine responsible for equal distribution of chromosomes between the daughter cells. RPE1 and U2OS cells were visualized and analyzed. In this thesis the protocol for expansion micoscropy was developed and used for imaging the whole mitotic spindle in human cells. It was shown that the images of the expanded mitotic spindles offer plenty information about the architecture of the spindle and in the future it would be interesting to optimize the protocol for visualising any protein in the mitotic spindle with the expansion microscopy.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:502098
Study programmeTitle: Integrated undergraduate and graduate university study of Biology and Chemistry Education; specializations in: Integrated undergraduate and graduate university study of Biology and Chemistry Education
Course: Integrated undergraduate and graduate university study of Biology and Chemistry Education
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: magistar/magistra edukacije biologije i kemije (magistar/magistra edukacije biologije i kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2020-10-01
Terms of useLicence In copyright icon
Created on2018-10-29 12:04:50