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master's thesis
Uloga proteina NuMa u sastavljanju i arhitekturi mitotskog diobenog vretena
Zagreb: University of Zagreb, Faculty of Science, 2018. urn:nbn:hr:217:280143
Jelenić, Stela
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Jelenić, S. (2018). Uloga proteina NuMa u sastavljanju i arhitekturi mitotskog diobenog vretena (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:280143

Jelenić, Stela. "Uloga proteina NuMa u sastavljanju i arhitekturi mitotskog diobenog vretena." Master's thesis, University of Zagreb, Faculty of Science, 2018. https://urn.nsk.hr/urn:nbn:hr:217:280143

Jelenić, Stela. "Uloga proteina NuMa u sastavljanju i arhitekturi mitotskog diobenog vretena." Master's thesis, University of Zagreb, Faculty of Science, 2018. https://urn.nsk.hr/urn:nbn:hr:217:280143

Jelenić, S. (2018). 'Uloga proteina NuMa u sastavljanju i arhitekturi mitotskog diobenog vretena', Master's thesis, University of Zagreb, Faculty of Science, accessed 29 December 2024, https://urn.nsk.hr/urn:nbn:hr:217:280143

Jelenić S. Uloga proteina NuMa u sastavljanju i arhitekturi mitotskog diobenog vretena [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2018 [cited 2024 December 29] Available at: https://urn.nsk.hr/urn:nbn:hr:217:280143

S. Jelenić, "Uloga proteina NuMa u sastavljanju i arhitekturi mitotskog diobenog vretena", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:217:280143

Metadata
TitleUloga proteina NuMa u sastavljanju i arhitekturi mitotskog diobenog vretena
Title (english)The role of NuMA in mitotic spindle assembly and architecture
AuthorStela Jelenić
MentorIva Tolić (mentor)
MentorMaja Matulić (mentor)
Committee memberMaja Matulić (predsjednik povjerenstva)
Committee memberMartina Šeruga Musić (član povjerenstva)
Committee memberSofia Ana Blažević (član povjerenstva)
Committee memberBiljana Balen (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2018-09-17, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Stanica tijekom mitoze stvara diobeno vreteno: dinamičnu i kompleksnu strukturu sastavljenu od mikrotubula, motornih i nemotornih proteina. Nemotorni protein NuMA (eng. nuclear and mitotic apparatus) tijekom mitoze je uglavnom prisutan na polovima vretena gdje sudjeluje u njihovom održavanju i u manjoj mjeri na staničnom korteksu u kompleksu s proteinom LGN gdje sudjeluje u pozicioniranju diobenog vretena unutar stanice. U ovome radu promatrana je njegova uloga u mitozi kada je utišana ekspresija proteina LGN pomoću siRNA u stanicama linije HeLa. Prilikom utišavanja došlo je do promjene arhitekture diobenog vretena i smanjenja heliciteta njegovih mikrotubula, međutim, lokalizacija proteina NuMA i polovi diobenog vretena su ostali očuvani. Također, konstruiran je optogenetički sustav za protein NuMA koji, iako se pokazao funkcionalnim, nije bio dovoljno efikasan da bi se iskoristio za detaljno određivanje njegove funkcije u metafazi mitoze. Ipak, moguće je da bi ovaj sustav bio iznimno moćan alat u drugoj staničnoj liniji ili drugoj fazi mitoze. Na temelju dobivenih rezultata može se zaključiti da protein LGN najvjerojatnije ima dodatnu funkciju tijekom mitoze vezanu uz diobeno vreteno koja vjerojatno ne ovisi o njegovoj interakciji s proteinom NuMA.
Abstract (english)
During mitosis, the cells create a dynamic and complex structure called mitotic spindle made of microtubules, motor and crosslinking proteins. NuMA (Nuclear and Mitotic Apparatus) is a crosslinking protein which is, during mitosis, mostly present on spindle poles maintaining them and in a smaller amount on the cell cortex in complex with LGN protein which is responsible for spindle orientation. In this thesis the role of NuMA in mitosis was investigated in cells in which LGN protein expression had been silenced by siRNA in HeLa cells. The result of silencing was change in spindle architecture and decrease in the helicity of microtubule bundles, but NuMA localization and spindle poles were preserved. Furthermore, an optogenetic system for NuMA was constructed. It was shown to be functional, but not efficient enough to investigate NuMA’s function in the metaphase of mitosis. Nevertheless, the constructed system could be a very powerful tool in a different cell line or in another phase of mitosis. It could be concluded that LGN protein has, beside NuMA regulation, an additional function in mitosis that is connected to the spindle and probably does not depend on interaction with NuMA.
Keywords
Languagecroatian
URN:NBNurn:nbn:hr:217:280143
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2020-09-17
Terms of useLicence In copyright icon
Created on2018-10-29 13:26:11