prikaz prve stranice dokumenta Karakterizacija adhesoma u stanicama karcinoma pločastog epitela jezika Cal27 nakon de novo ekspresije integrina αvß3
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master's thesis
Karakterizacija adhesoma u stanicama karcinoma pločastog epitela jezika Cal27 nakon de novo ekspresije integrina αvß3
Zagreb: University of Zagreb, Faculty of Science, 2018. urn:nbn:hr:217:212111
Čuljak, Kristina
University of Zagreb
Faculty of Science
Department of Chemistry

Institutional repository: Repository of the Faculty of Science

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Čuljak, K. (2018). Karakterizacija adhesoma u stanicama karcinoma pločastog epitela jezika Cal27 nakon de novo ekspresije integrina αvß3 (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:212111

Čuljak, Kristina. "Karakterizacija adhesoma u stanicama karcinoma pločastog epitela jezika Cal27 nakon de novo ekspresije integrina αvß3." Master's thesis, University of Zagreb, Faculty of Science, 2018. https://urn.nsk.hr/urn:nbn:hr:217:212111

Čuljak, Kristina. "Karakterizacija adhesoma u stanicama karcinoma pločastog epitela jezika Cal27 nakon de novo ekspresije integrina αvß3." Master's thesis, University of Zagreb, Faculty of Science, 2018. https://urn.nsk.hr/urn:nbn:hr:217:212111

Čuljak, K. (2018). 'Karakterizacija adhesoma u stanicama karcinoma pločastog epitela jezika Cal27 nakon de novo ekspresije integrina αvß3', Master's thesis, University of Zagreb, Faculty of Science, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:212111

Čuljak K. Karakterizacija adhesoma u stanicama karcinoma pločastog epitela jezika Cal27 nakon de novo ekspresije integrina αvß3 [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2018 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:217:212111

K. Čuljak, "Karakterizacija adhesoma u stanicama karcinoma pločastog epitela jezika Cal27 nakon de novo ekspresije integrina αvß3", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:217:212111

Metadata
TitleKarakterizacija adhesoma u stanicama karcinoma pločastog epitela jezika Cal27 nakon de novo ekspresije integrina αvß3
Title (english)Characterisation of adhesome in tongue squamous carcinoma cells Cal27 upon de novo expression of integrin αvß3
AuthorKristina Čuljak
MentorAndreja Ambriović Ristov (mentor)
Committee memberMorana Dulić (predsjednik povjerenstva)
Committee memberŽeljka Soldin (član povjerenstva)
Committee memberIva Juranović Cindrić (član povjerenstva)
Committee memberBiserka Prugovečki (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Chemistry)
Zagreb
Defense date and country2018-09-26, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
Vezanjem na izvanstanični matriks, integrini regrutiraju raznovrsne proteine oko citoplazmatskog repa stvarajući strukture poznate kao fokalne adhezije. Skup svih molekula povezanih s adhezijom integrina naziva se adhesom integrina. U ovom smo radu okarakterizirali adhesome stanica pločastog epitela jezika Cal27 i klona Cal27-2B1, dobivenog transfekcijom stanica Cal27 plazmidom koji sadrži cDNA za podjedinicu integrina β3 i za kojeg je pokazana otpornost na nekoliko protutumorskih lijekova i povećana pokretljivost. Izolirani su kompleksi adhezije integrina stanica Cal27 i Cal27-2B1 i analizirani pomoću spektrometrije masa. Otkriveno je 639 različitih proteina od kojih je 19 dio konsenzus adhesoma. U Cal27-2B1 otkriveno je 17 proteina koji se nalaze u većim količinama nego u stanicama Cal27. Ovaj rad pruža vrijedan izvor informacija o sastavu adhesoma karcinoma pločastog epitela jezika i promjenama u signalnim putevima integrina koje su potencijalno uključene u fenotip povećane otpornosti na protutumorske lijekove i povećanu pokretljivost čime bi se mogle otkriti nove mogućnosti za terapiju tumora.
Abstract (english)
Upon ligation to the extracellular matrix, integrins recruit various proteins to form structures known as focal adhesions. The entire collective of molecules associated with integrin adhesions was termed the integrin adhesome. In the present study we characterised adhesomes of tongue squamous carcinoma cells Cal27 and Cal27-derived clone Cal27-2B1, obtained by transfection of plasmid containing integrin subunit β3 cDNA and showing resistance to several antitumor drugs, increased migration and invasion. The integrin adehesion complexes from Cal27 and Cal27-2B1 were isolated and analysed using mass spectrometry identifying 639 different proteins with 19 out of 60 consensus adhesome proteins. In clone Cal27-2B1, 17 proteins were detected at much higher levels compared with Cal27 cells providing a valuable resource of information that might reveal novel opportunities for therapeutic intervention in tumors.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:212111
Study programmeTitle: Graduate university studies in chemistry; specializations in: Research programme
Course: Research programme
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2020-09-26
Terms of useLicence In copyright icon
Created on2018-11-19 13:00:48