prikaz prve stranice dokumenta Razvoj metode tekućinske kromatografije ultravisoke djelotvornosti za određivanje onečišćenja u ceritinibu
  Download
PDF 1.23 MB
master's thesis
Razvoj metode tekućinske kromatografije ultravisoke djelotvornosti za određivanje onečišćenja u ceritinibu
Zagreb: University of Zagreb, Faculty of Science, 2018. urn:nbn:hr:217:853147
Mašić, Borna
University of Zagreb
Faculty of Science
Department of Chemistry

Institutional repository: Repository of the Faculty of Science

Cite this document

Mašić, B. (2018). Razvoj metode tekućinske kromatografije ultravisoke djelotvornosti za određivanje onečišćenja u ceritinibu (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:853147

Mašić, Borna. "Razvoj metode tekućinske kromatografije ultravisoke djelotvornosti za određivanje onečišćenja u ceritinibu." Master's thesis, University of Zagreb, Faculty of Science, 2018. https://urn.nsk.hr/urn:nbn:hr:217:853147

Mašić, Borna. "Razvoj metode tekućinske kromatografije ultravisoke djelotvornosti za određivanje onečišćenja u ceritinibu." Master's thesis, University of Zagreb, Faculty of Science, 2018. https://urn.nsk.hr/urn:nbn:hr:217:853147

Mašić, B. (2018). 'Razvoj metode tekućinske kromatografije ultravisoke djelotvornosti za određivanje onečišćenja u ceritinibu', Master's thesis, University of Zagreb, Faculty of Science, accessed 20 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:853147

Mašić B. Razvoj metode tekućinske kromatografije ultravisoke djelotvornosti za određivanje onečišćenja u ceritinibu [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2018 [cited 2024 November 20] Available at: https://urn.nsk.hr/urn:nbn:hr:217:853147

B. Mašić, "Razvoj metode tekućinske kromatografije ultravisoke djelotvornosti za određivanje onečišćenja u ceritinibu", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:217:853147

Metadata
TitleRazvoj metode tekućinske kromatografije ultravisoke djelotvornosti za određivanje onečišćenja u ceritinibu
Title (english)Development of ultra performance liquid chromatographic method for determination of impurities in the ceritinib
AuthorBorna Mašić
MentorNives Galić (mentor)
Committee memberNives Galić (predsjednik povjerenstva)
Committee memberŽeljka Soldin (član povjerenstva)
Committee memberMorana Dulić (član povjerenstva)
Committee memberSnežana Miljanić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Chemistry)
Zagreb
Defense date and country2018-09-28, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
U okviru diplomskog rada razvijena je metoda za kvantitativno određivanje onečišćenja ceritiniba primjenom kromatografije ultravisoke djelotvornosti uz korištenje programa Drylab za optimizaciju parametara. Konačna metoda prilagođena je vezanom sustavu kromatografije visoke djelotovornosti spregnutom sa spektrometrijom masa kako bi se potvrdila masa onečišćenja. Pri razvoju metode proveden je niz eksperimenata na dvije različite kolone, C18 i BEH Phenyl, pri temperaturama od 30 °C i 60 °C, tri pH-vrijednosti 2,0; 4,0; 6,0 te dva različita gradijenta u trajanju 13 i 25 minute. Apsorpcija je praćena pri valnim duljinama od 220 nm, 240 nm i 270 nm. Uz navedene parametre također su korištene različite kombinacije vodenih i organskih faza. Rezultati eksperimenata su analizirani uz pomoć DryLab programa i prilagođeni uvjetima realnog sustava kako bi se dobili konačni uvjeti metode. Odabrana kolona je BEH Phenyl, temperatura 55 °C, gradijent u trajanju 17 minuta, valna duljina 210 nm, pufer KH2PO4 pH vrijednosti 4,3. Kao organska faza odabran je acetonitril.
Abstract (english)
In this diploma thesis, a quantitative method for determination of ceritinib impurities by ultra performance liquid chromatography using Drylab program for parameter optimization was developed. The method was adapted for use in high-performance chromatography systems coupled with mass spectrometry to confirm the mass of the aforementioned impurities. The process of parameter optimization was performed via series of experiments in which two types of columns were used, C18 and BEH Phenyl, at temperatures of 30 °C and 60 °C, three pH values 2.0; 4.0; 6.0; at two different gradients in duration of 13 and 25 minutes. Chromatograms were recorded at wavelengths of 220 nm, 240 nm and 270 nm. Different combinations of organic and water phases were also used. The results of the experiments were analyzed with DryLab software and the final conditions of the method were established: BEH Phenyl column, system temperature of 55 °C, 17 minute gradient, a wavelength of 210 nm, buffer KH2PO4 pH value 4.3. Acetonitrile was used as the organic phase.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:853147
Study programmeTitle: Graduate university studies in chemistry; specializations in: Research programme
Course: Research programme
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2020-09-28
Terms of useLicence In copyright icon
Created on2018-11-26 10:48:55