prikaz prve stranice dokumenta Karakterizacija adhesoma u stanicama trostruko negativnog karcinoma dojke MDA-MB-231 nakon stabilnog utišavanja gena za integrin alfav
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master's thesis
Karakterizacija adhesoma u stanicama trostruko negativnog karcinoma dojke MDA-MB-231 nakon stabilnog utišavanja gena za integrin alfav
Zagreb: University of Zagreb, Faculty of Science, 2018. urn:nbn:hr:217:784433
Vitković, Zrinka
University of Zagreb
Faculty of Science
Department of Chemistry

Institutional repository: Repository of the Faculty of Science

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Vitković, Z. (2018). Karakterizacija adhesoma u stanicama trostruko negativnog karcinoma dojke MDA-MB-231 nakon stabilnog utišavanja gena za integrin alfav (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:784433

Vitković, Zrinka. "Karakterizacija adhesoma u stanicama trostruko negativnog karcinoma dojke MDA-MB-231 nakon stabilnog utišavanja gena za integrin alfav." Master's thesis, University of Zagreb, Faculty of Science, 2018. https://urn.nsk.hr/urn:nbn:hr:217:784433

Vitković, Zrinka. "Karakterizacija adhesoma u stanicama trostruko negativnog karcinoma dojke MDA-MB-231 nakon stabilnog utišavanja gena za integrin alfav." Master's thesis, University of Zagreb, Faculty of Science, 2018. https://urn.nsk.hr/urn:nbn:hr:217:784433

Vitković, Z. (2018). 'Karakterizacija adhesoma u stanicama trostruko negativnog karcinoma dojke MDA-MB-231 nakon stabilnog utišavanja gena za integrin alfav', Master's thesis, University of Zagreb, Faculty of Science, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:784433

Vitković Z. Karakterizacija adhesoma u stanicama trostruko negativnog karcinoma dojke MDA-MB-231 nakon stabilnog utišavanja gena za integrin alfav [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2018 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:217:784433

Z. Vitković, "Karakterizacija adhesoma u stanicama trostruko negativnog karcinoma dojke MDA-MB-231 nakon stabilnog utišavanja gena za integrin alfav", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:217:784433

Metadata
TitleKarakterizacija adhesoma u stanicama trostruko negativnog karcinoma dojke MDA-MB-231 nakon stabilnog utišavanja gena za integrin alfav
Title (english)Adhesome characterisation in triple negative breast carcinoma cell line MDA-MB-231 upon stable silencing of integrin subbunit alphav
AuthorZrinka Vitković
MentorAndreja Ambriović Ristov (mentor)
Committee memberMarko Močibob (predsjednik povjerenstva)
Committee memberBiserka Prugovečki (član povjerenstva)
Committee memberIva Juranović Cindrić (član povjerenstva)
Committee memberMorana Dulić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Chemistry)
Zagreb
Defense date and country2018-10-29, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
Adhesom integrina je skup proteina koje čine kompleksi adhezije integrina neke stanice. Ovi kompleksi se nazivaju i fokalne adhezije, a nastaju kada se integrini, transmembranski receptori sastavljeni od α i β podjedinice, vežu na izvanstanični matriks, što dovodi do nakupljanja proteina vezanjem na njihovu citoplazmatsku domenu. Cilj je ovog rada bio metodom spektrometrije masa istražiti adhesom stanica trostruko negativnog karcinoma dojke MDAMB- 231 i usporediti ga s adhesomom stanica klona 231shαv u kojima je, pomoću shRNA specifične za podjedinicu integrina αv, postignuto stabilno utišavanje te podjedinice. Nakon izolacije proteina fokalnih adhezija, spektrometrijom masa identificirano je 426 proteina, a bioinformatičkom analizom utvrđeno je da je njih 107 povezano s fokalnim adhezijama, a 95 s izvanstaničnim matriksom. Smanjena količina nekih od identificiranih proteina potvrđena je metodom western blot. U ovome radu definiran je adhesom stanica MDA-MB-231 ovisan o integrinu αv i doprinjet će otkrivanju mehanizma promjene osjetljivosti ovih stanica na protutumorske lijekove, cisplatinu i paklitaksel, kao i promjene u sposobnosti migracije i invazije ovih stanica nakon utišavanja integrina αv.
Abstract (english)
The integrin adhesome is a collection of proteins composed of integrin adhesion complexes in a given cell. These complexes are also known as focal adhesions which are formed when integrins, transmembrane receptors comprising an α and β subunit, bind the extracellular matrix and lead to accumulation of mutimolecular complex of proteins through binding to the cytoplasmic domains of integrins. The aim of this project was to explore by mass spectrometry, the adhesome of triple negative breast cancer cells MDA-MB-231 and compare it to the adhesome of 231shαv cells in which the αv integrin subunit has been silenced by the use of αv specific shRNA. After focal adhesion isolation, 426 proteins were identified using mass spectrometry, 107 of which were found to be related to focal adhesions, while 95 of them were found to be related to the extracellular matrix. Western blots were used to validate the expression of some of the identified proteins. This project has identified the adhesome of MDAMB- 231 cells and will contribute to research of the mechanism by which the sensitivity of these cells to cisplatin and paclitaxel is modulated, as well as their ability to modulate migration and invasion upon aα integrin knockdown.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:784433
Study programmeTitle: Graduate university studies in chemistry; specializations in: Research programme
Course: Research programme
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2020-10-29
Terms of useLicence In copyright icon
Created on2018-11-27 09:15:53