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doctoral thesis
Hidrazino-derivat glutaminske kiseline za derivatizaciju karbonilnih spojeva
Zagreb: University of Zagreb, Faculty of Science, 2018. urn:nbn:hr:217:659583
Brkljačić, Lidija
University of Zagreb
Faculty of Science
Department of Chemistry

Institutional repository: Repository of the Faculty of Science

Cite this document

Brkljačić, L. (2018). Hidrazino-derivat glutaminske kiseline za derivatizaciju karbonilnih spojeva (Doctoral thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:659583

Brkljačić, Lidija. "Hidrazino-derivat glutaminske kiseline za derivatizaciju karbonilnih spojeva." Doctoral thesis, University of Zagreb, Faculty of Science, 2018. https://urn.nsk.hr/urn:nbn:hr:217:659583

Brkljačić, Lidija. "Hidrazino-derivat glutaminske kiseline za derivatizaciju karbonilnih spojeva." Doctoral thesis, University of Zagreb, Faculty of Science, 2018. https://urn.nsk.hr/urn:nbn:hr:217:659583

Brkljačić, L. (2018). 'Hidrazino-derivat glutaminske kiseline za derivatizaciju karbonilnih spojeva', Doctoral thesis, University of Zagreb, Faculty of Science, accessed 21 December 2024, https://urn.nsk.hr/urn:nbn:hr:217:659583

Brkljačić L. Hidrazino-derivat glutaminske kiseline za derivatizaciju karbonilnih spojeva [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Science; 2018 [cited 2024 December 21] Available at: https://urn.nsk.hr/urn:nbn:hr:217:659583

L. Brkljačić, "Hidrazino-derivat glutaminske kiseline za derivatizaciju karbonilnih spojeva", Doctoral thesis, University of Zagreb, Faculty of Science, Zagreb, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:217:659583

Metadata
TitleHidrazino-derivat glutaminske kiseline za derivatizaciju karbonilnih spojeva
Title (english)Glutamic acid-derived hydrazine reagent for derivatization of carbonyl compounds
AuthorLidija Brkljačić
MentorIvanka Jerić (mentor)
Committee memberPredrag Novak (predsjednik povjerenstva)
Committee memberNives Galić (član povjerenstva)
Committee memberIvanka Jerić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Chemistry)
Zagreb
Defense date and country2018-09-26, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Universal decimal classification
(UDC)		54 - Chemistry. Crystallography. Mineralogy
Abstract
U okviru provedenih istraživanja osmišljen je i pripravljen novi reagens za derivatizaciju karbonilnih spojeva, hidrazino-derivat glutaminske kiseline, koji je upotrebljen za određivanje karbonilnih spojeva LC-MS/MS metodom. Istraživanje je obuhvatilo i njegovu karakterizaciju spektroskopskim i spektrometrijskim metodama. Reakcijom hidrazino-derivata glutaminske kiseline i odabranih karbonilnih spojeva dobiveni su Glu-hidrazoni, koji su prema potrebi reducirani u Glu-hidrazide. Nastali produkti su izolirani te okarakterizirani spektroskopskim i spektrometrijskim metodama. U doktorskom radu istražene su fragmentacijske reakcije nastalih hidrazona i hidrazida u MS/MS eksperimentima kako bi se identificirali specifični ioni produkti koji su upotrijebljeni za razvoj LC-MS/MS metode. Istraženi su i optimirani reakcijski uvjeti koji su osigurali kvantitativnost derivatizacijske reakcije. Za usporedbu, sintetizirani su i okarakterizirani produkti odabranih karbonilnih spojeva s najčešće korištenim reagensom za derivatizaciju karbonilnih spojeva, 2,4 dinitrofenilhidrazinom.
Abstract (english)
The scope of the study included the design and preparation of a glutamic acid-related hydrazine reagent as new reagent for the derivatization of carbonyl compounds for LC-MS/MS analysis and its characterization by spectroscopic and spectrometric methods. Reaction of the glutamic acid-related hydrazine reagent and the selected carbonyls resulted in the corresponding Glu-hydrazones which were optionally reduced to Glu-hydrazides. The formed products were isolated and characterized by spectroscopic and spectrometric methods. Fragmentation patterns of prepared hydrazones and hydrazides in MS/MS experiments were also investigated for the identification of “marker ions”, used for the development of LC-MS/MS method. The optimization of reaction conditions was performed to provide a quantitative derivatization. For comparison purposes, products of selected carbonyls with the most exploited derivatization reagent 2,4-dinitrophenylhydrazine, were also synthesized and characterized.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:659583
Study programmeTitle: Doctoral study in chemistry
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje kemija (doktor/doktorica znanosti, područje prirodnih znanosti, polje kemija)
Type of resourceText
Extent108 str. ; 30 cm
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2018-11-27 13:34:49