prikaz prve stranice dokumenta Structural and functional analysis of hybrid proteasome Strukturna i funkcionalna analiza hibridnog proteasoma
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master's thesis
Structural and functional analysis of hybrid proteasome Strukturna i funkcionalna analiza hibridnog proteasoma
Zagreb: University of Zagreb, Faculty of Science, 2018. urn:nbn:hr:217:182054
Belačić, Katarina
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Belačić, K. (2018). Structural and functional analysis of hybrid proteasome Strukturna i funkcionalna analiza hibridnog proteasoma (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:182054

Belačić, Katarina. "Structural and functional analysis of hybrid proteasome Strukturna i funkcionalna analiza hibridnog proteasoma." Master's thesis, University of Zagreb, Faculty of Science, 2018. https://urn.nsk.hr/urn:nbn:hr:217:182054

Belačić, Katarina. "Structural and functional analysis of hybrid proteasome Strukturna i funkcionalna analiza hibridnog proteasoma." Master's thesis, University of Zagreb, Faculty of Science, 2018. https://urn.nsk.hr/urn:nbn:hr:217:182054

Belačić, K. (2018). 'Structural and functional analysis of hybrid proteasome Strukturna i funkcionalna analiza hibridnog proteasoma', Master's thesis, University of Zagreb, Faculty of Science, accessed 03 January 2025, https://urn.nsk.hr/urn:nbn:hr:217:182054

Belačić K. Structural and functional analysis of hybrid proteasome Strukturna i funkcionalna analiza hibridnog proteasoma [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2018 [cited 2025 January 03] Available at: https://urn.nsk.hr/urn:nbn:hr:217:182054

K. Belačić, "Structural and functional analysis of hybrid proteasome Strukturna i funkcionalna analiza hibridnog proteasoma", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:217:182054

Metadata
TitleStructural and functional analysis of hybrid proteasome Strukturna i funkcionalna analiza hibridnog proteasoma
Title (croatian)Strukturna i funkcionalna analiza hibridnog proteasoma
AuthorKatarina Belačić
MentorDavid Haselbach (mentor)
MentorNenad Malenica (mentor)
Committee memberNenad Malenica (predsjednik povjerenstva)
Committee memberAntun Alegro (član povjerenstva)
Committee memberMarin Ježić (član povjerenstva)
Committee memberInga Marijanović (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2018-11-23, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Proteasomes perform the majority of proteolysis that occurs in cells, and thereby, perform crucial roles in regulation of cellular processes such as cell division and death, signal transduction, immune response, metabolism and protein quality control. The catalytic heart of proteasome complexes is the 20S core particle (20S CP) which activity is regulated through association with different activators – 19S regulatory particle (19SRP), PA28ab, PA28g and PA200. The hybrid proteasome complex contains the 19S RP on the one side and PA28 or PA200 on the opposite side of the same 20S CP. The hybrid proteasome complexes can be found under native conditions, or their formation can be induced (e.g., by interferon-γ treatment). The exact function of hybrid proteasomes is still unknown and their structures have never been thoroughly investigated. The structural analysis of hybrid proteasome complexes from bovine testes and interferon-g treated human rectal carcinoma cells by single particle cryo-electron microscopy has resulted only in the structures of the PA200 hybrid proteasome complex, however at a too low resolution for reliable structural insights. Meanwhile, the peptide pattern in the presence of 19S-20S-PA28ab or 19S-20S-PA28g complexes was found to be changed in comparison with the one obtained without PA28 complexes, indicating that PA28 allosterically regulates the cleavage specificities of 19S-20S proteasome complex.
Abstract (croatian)
Proteasom je proteinski kompleks zadužen za razgradnju proteina unutar stanice te je stoga važan za regulaciju staničnih procesa – diobu i smrt stanice, prijenos signala, imunološki odgovor, metabolizam, provjeru kvalitete proteina i razvoj. Katalitički aktivan dio čini 20S kompleks čija je aktivnost regulirana vezanjem različitih aktivatora poput 19S regulatornog kompleksa (19S RP), PA28ab, PA28g te PA200. Hibridni proteasom sadrži 19S RP na jednoj i PA28 ili PA200 na drugoj strani istog 20S kompleksa. Hibridni proteasomi mogu biti prisutni u normalnim uvjetima ili njihov nastanak može biti potaknut (npr. stimulacijom interferonom- γ). Međutim, točna funkcija hibridnih proteasoma je još uvijek nepoznata, a struktura nikada nije detaljno istraživana. Strukturna analiza krio-elektronskom mikroskopijom pojedinačnih čestica hibridnih proteasoma izoliranih iz tkiva goveđih testisa i stanica ljudskog rektalnog karcionoma tretiranih interferonom- γ pokazala je prisutnost samo hibridnih proteasoma koji sadrže PA200 aktivator. Međutim, zbog preniske rezolucije nisu dobivene pouzdane strukturne informacije. Također, pronađeno je da se u prisutnosti kompleksa 19S-20S-PA28ab ili 19S- 20S-PA28g, uzorak peptidnih fragmenata odabranog proteinskog supstrata razlikuje od uzorka dobivenog u odustnosti PA28 aktivatora. Takav rezultat ukazuje na to da PA28 aktivator alosterički regulira specifičnosti proteolitičkog cijepanja 20S-19S kompleksa.
Keywords
Keywords (croatian)
Languageenglish
URN:NBNurn:nbn:hr:217:182054
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2019-11-23
Terms of useLicence In copyright icon
Created on2018-12-04 11:48:12