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master's thesis
Utjecaj salinomicina na N-glikom u modelu tumorskih matičnih stanica
Zagreb: University of Zagreb, Faculty of Science, 2018. urn:nbn:hr:217:497797
Kliček, Filip
University of Zagreb
Faculty of Science
Department of Chemistry

Institutional repository: Repository of the Faculty of Science

Cite this document

Kliček, F. (2018). Utjecaj salinomicina na N-glikom u modelu tumorskih matičnih stanica (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:497797

Kliček, Filip. "Utjecaj salinomicina na N-glikom u modelu tumorskih matičnih stanica." Master's thesis, University of Zagreb, Faculty of Science, 2018. https://urn.nsk.hr/urn:nbn:hr:217:497797

Kliček, Filip. "Utjecaj salinomicina na N-glikom u modelu tumorskih matičnih stanica." Master's thesis, University of Zagreb, Faculty of Science, 2018. https://urn.nsk.hr/urn:nbn:hr:217:497797

Kliček, F. (2018). 'Utjecaj salinomicina na N-glikom u modelu tumorskih matičnih stanica', Master's thesis, University of Zagreb, Faculty of Science, accessed 09 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:497797

Kliček F. Utjecaj salinomicina na N-glikom u modelu tumorskih matičnih stanica [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2018 [cited 2024 November 09] Available at: https://urn.nsk.hr/urn:nbn:hr:217:497797

F. Kliček, "Utjecaj salinomicina na N-glikom u modelu tumorskih matičnih stanica", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:217:497797

Metadata
TitleUtjecaj salinomicina na N-glikom u modelu tumorskih matičnih stanica
Title (english)Effect of salinomycin on N-glycome in a cancer stem cell model
AuthorFilip Kliček
MentorGordan Lauc (mentor)
Committee memberMorana Dulić (predsjednik povjerenstva)
Committee memberNives Galić (član povjerenstva)
Committee memberBiserka Prugovečki (član povjerenstva)
Committee memberMarko Močibob (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Chemistry)
Zagreb
Defense date and country2018-12-12, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
N-glikozilacija je post-translacijska modifikacija proteina koja se odvija u endoplazmatskom retikulumu i Golgijevom aparatu. Modifikacije N-glikana u Golgijevom aparatu su pH ovisne te je narušavanje homeostaze pH u Golgijevom aparatu vidljivo u promjeni struktura Nglikana. Efekt salinomicina istraživan je na profilu N-glikana na modelu TMS (HMLE-pBp i HMLE-Twist). Za salinomicin je prethodno pokazano selektivno djelovanje na tumorske matične stanice (TMS). Profil N-glikoma sekretiranih proteina se promijenio nakon tretmana salinomicinom u obje stanične linije. Najuočljivija razlika u profilima nakon tretmana je izostanak razgranatih kompleksnih glikanskih struktura te relativno povećanje jednostavnih glikana, što upućuje na to da salinomicin narušava homeostazu u Golgijevom aparatu dok endoplazmatski retikulum ostaje nepromijenjen. Nadalje, primijetili samo da je efekt naglašeniji na HMLE-Twist nego na HMLE-pBp staničnoj liniji.
Abstract (english)
N-glycosilation is a post-translational modification of proteins that occurs in endoplasmatic reticulum and Golgi apparatus. Modifications of N-glycans in the Golgi apparatus are pH dependent and a disruption of pH homeostasis in Golgi apparatus is visible in the change of N-glycan structures. We investigated the effect of salinomycin, a cancer stem cell (CSC) targeting compound, on N-glycan profile of a CSC model (HMLE-pBp and HMLE-Twist cell lines). N-glycome profile of secreted proteins changed in both cell lines after treatment with salinomycin. The most evident difference in profiles after treatment is the loss of highly branched complex glycan structures and the relative increase of simpler glycans, indicating that salinomycin disrupts the Golgi apparatus homeostasis while the endoplasmatic reticulum remains intact. Moreover, we noticed that the effect of is more prominent on HMLE-Twist than on HMLE-pBp cell line.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:497797
Study programmeTitle: Graduate university studies in chemistry; specializations in: Research programme
Course: Research programme
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2020-12-12
Terms of useLicence In copyright icon
Created on2018-12-14 13:18:39