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master's thesis
Analiza nadekspresorskih linija uročnjaka (Arabidopsis thaliana L.) za auksin-amidohidrolazu AtILL2
Zagreb: University of Zagreb, Faculty of Science, 2019. urn:nbn:hr:217:865119
Mikelić, Ana
University of Zagreb
Faculty of Science
Department of Chemistry

Institutional repository: Repository of the Faculty of Science

Cite this document

Mikelić, A. (2019). Analiza nadekspresorskih linija uročnjaka (Arabidopsis thaliana L.) za auksin-amidohidrolazu AtILL2 (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:865119

Mikelić, Ana. "Analiza nadekspresorskih linija uročnjaka (Arabidopsis thaliana L.) za auksin-amidohidrolazu AtILL2." Master's thesis, University of Zagreb, Faculty of Science, 2019. https://urn.nsk.hr/urn:nbn:hr:217:865119

Mikelić, Ana. "Analiza nadekspresorskih linija uročnjaka (Arabidopsis thaliana L.) za auksin-amidohidrolazu AtILL2." Master's thesis, University of Zagreb, Faculty of Science, 2019. https://urn.nsk.hr/urn:nbn:hr:217:865119

Mikelić, A. (2019). 'Analiza nadekspresorskih linija uročnjaka (Arabidopsis thaliana L.) za auksin-amidohidrolazu AtILL2', Master's thesis, University of Zagreb, Faculty of Science, accessed 14 May 2024, https://urn.nsk.hr/urn:nbn:hr:217:865119

Mikelić A. Analiza nadekspresorskih linija uročnjaka (Arabidopsis thaliana L.) za auksin-amidohidrolazu AtILL2 [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2019 [cited 2024 May 14] Available at: https://urn.nsk.hr/urn:nbn:hr:217:865119

A. Mikelić, "Analiza nadekspresorskih linija uročnjaka (Arabidopsis thaliana L.) za auksin-amidohidrolazu AtILL2", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:217:865119

Metadata
TitleAnaliza nadekspresorskih linija uročnjaka (Arabidopsis thaliana L.) za auksin-amidohidrolazu AtILL2
Title (english)Analysis of auxin amidohydrolase AtILL2 overexpressor lines in Arabidopsis thaliana L.
AuthorAna Mikelić
MentorBranka Salopek Sondi (mentor)
Committee memberAleksandra Maršavelski (predsjednik povjerenstva)
Committee memberIva Juranović Cindrić (član povjerenstva)
Committee memberDavor Kovačević (član povjerenstva)
Committee memberMorana Dulić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Chemistry)
Zagreb
Defense date and country2019-02-27, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
Biljni hormoni auksini imaju važnu ulogu u biljnom rastu i razvitku te u odgovoru biljaka na čimbenike stresa. Aktivan hormon oslobađa se iz konjugata s aminokiselinama djelovanjem enzima auksin-amidohidrolaza koje sudjeluju u homeostazi auksina. Svrha ovog rada bila je istražiti ulogu auksin-amidohidrolaze AtILL2 iz uročnjaka (Arabidopsis thaliana) u odgovoru biljaka na solni i osmotski stres. Analizirane su četiri homozigotne linije A. thaliana ekotipa Ws potencijalnih nadekspresora za fuzijski protein AtILL2 s GFP- i/ili His-privjeskom. Prisutnost transgena provjerena je na razini insercije u genomsku DNA, prisutnosti RNA transkripta i rekombinantnog proteina korištenjem slijedećih metoda: PCR, RT-PCR, SDS-PAGE, afinitetna kromatografija i western-hibridizacijska analiza. U tri linije potvrđena je prisutnost transgena na razini insercije u genomsku DNA i transkripcije u RNA iako nije dokazana prisutnost rekombinantnih proteina niti u jednoj liniji. Testom inhibicije rasta korijena dokazana je povećana otpornost jedne linije na povišeni salinitet i osmolarnost.
Abstract (english)
Phytohormones auxins play an important role in plant growth development and abiotic stress responses. The active auxin may be released from the amino acid conjugates by the action of auxin-amidohydrolase enzymes, which thereby participate in the auxin homeostasis. The aim of this study was to investigate the role of auxin-amidohydrolase AtILL2 from Arabidopsis thaliana in the plant response to salinity and osmotic stress. Four homozygous lines of A. thaliana ecotypes Ws of potential overexpressors for AtILL2 fusion protein with GFP and / or His tags were analyzed. The presence of transgene was verified as insertion into DNA, RNA transcript, and recombinant protein by using PCR, RT-PCR, SDS-PAGE, affinity chromatography and western-hybridization assay. In three lines, the presence of transgene in genomic DNA and RNA transcript was confirmed, although the recombinant protein was not demonstrated in any of the lines. Root growth bioassay confirmed the increased resistance of one line to the examined stressors.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:865119
Study programmeTitle: Graduate university studies in chemistry; specializations in: Research programme
Course: Research programme
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2019-03-13 16:56:29