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master's thesis
Oksidacijski stres u biljkama duhana (Nicotiana tabacum L.) izloženim nanočesticama srebra
Zagreb: University of Zagreb, Faculty of Science, 2017. urn:nbn:hr:217:667937
Bajan, Željka
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Bajan, Ž. (2017). Oksidacijski stres u biljkama duhana (Nicotiana tabacum L.) izloženim nanočesticama srebra (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:667937

Bajan, Željka. "Oksidacijski stres u biljkama duhana (Nicotiana tabacum L.) izloženim nanočesticama srebra." Master's thesis, University of Zagreb, Faculty of Science, 2017. https://urn.nsk.hr/urn:nbn:hr:217:667937

Bajan, Željka. "Oksidacijski stres u biljkama duhana (Nicotiana tabacum L.) izloženim nanočesticama srebra." Master's thesis, University of Zagreb, Faculty of Science, 2017. https://urn.nsk.hr/urn:nbn:hr:217:667937

Bajan, Ž. (2017). 'Oksidacijski stres u biljkama duhana (Nicotiana tabacum L.) izloženim nanočesticama srebra', Master's thesis, University of Zagreb, Faculty of Science, accessed 25 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:667937

Bajan Ž. Oksidacijski stres u biljkama duhana (Nicotiana tabacum L.) izloženim nanočesticama srebra [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2017 [cited 2024 November 25] Available at: https://urn.nsk.hr/urn:nbn:hr:217:667937

Ž. Bajan, "Oksidacijski stres u biljkama duhana (Nicotiana tabacum L.) izloženim nanočesticama srebra", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:217:667937

Metadata
TitleOksidacijski stres u biljkama duhana (Nicotiana tabacum L.) izloženim nanočesticama srebra
Title (english)Oxidative stress in tobacco plants (Nicotiana tabacum L.) exposed to silver nanoparticles
AuthorŽeljka Bajan
MentorBiljana Balen (mentor)
Committee memberBiljana Balen (predsjednik povjerenstva)
Committee memberInes Radanović (član povjerenstva)
Committee memberVesna Petrović Peroković (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2017-02-24, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Nanočestice srebra (AgNP) dominiraju među vrstama dostupnih nanomaterijala zbog dobro poznatog protubakterijskog i protugljivičnog učinka srebra. Stoga se danas intenzivno ispituje njihov učinak na okoliš i žive organizme. U ovom radu je istražen utjecaj AgNP na pojavu oksidacijskog stresa u odraslim biljkama duhana (Nicotiana tabacum L.), a učinci nanosrebra uspoređeni su s učinkom ionskog srebra (AgNO3) istih koncentracija. Tretmani s AgNP nisu izazvali pojavu oksidacijskog stresa u korijenu i listu duhana jer nije zabilježena promjena u sadržaju reaktivnih oblika kisika (ROS), malondialdehida, proteinskih karbonila i oštećenju molekule DNA. Oksidacijski stres zabillježen je nakon tretmana biljaka s AgNO3 u korijenu prema svim ispitanim parametrima, a u listu prema većini parametara, što je bilo osobito izraženo pri najvećim ispitanim koncentracijama. Veće promjene u aktivnosti i ekspresiji antioksidacijskih enzima SOD, PPX, APX i CAT izmjerene su u korijenju i listovima duhana nakon izlaganja tretmanima s AgNO3 u odnosu na AgNP. Ionsko srebro pokazalo se toksičnije za biljke duhana od nanosrebra, a jači učinci zabilježeni su u korijenju u odnosu na listove.
Abstract (english)
Silver nanoparticles (AgNP) dominate among the types of available nanomaterials because of the well-known antibacterial and antifungal effects of silver. Therefore, their impact on the environment and living organisms intensively studied. This diploma thesis investigates the impact of AgNPs on the appereance of oxidative stress in adult tobacco plants (Nicotiana tabacum L.). The effects of nanosilver are compared with the effects of ionic silver (AgNO3), applied in the same concentration. AgNPs didn't induce oxidative stress since no changes were recorded in the content of reactive oxygen species (ROS), malondialdehyde, protein carbonyls and DNA damage in the roots and leaves of tobacco plants. However oxidative stress was recorded after the AgNO3 treatments, in the roots according to all tested parameters, and in the leaves according to majority of investigated parameters, which was particularly pronounced at the highest applied concentrations. Greater changes in the activity and expression of antioxidant enzymes SOD, PPX, APX and CAT were measured in the roots and leaves of tobacco after exposure to treatments with AgNO3 in relation to AgNP. Ionic silver proved to be more toxic to the tobacco plants compared to nanosilver and its stronger effects have been found in the roots compared to the leaves.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:667937
Study programmeTitle: Integrated undergraduate and graduate university study of Biology and Chemistry Education; specializations in: Integrated undergraduate and graduate university study of Biology and Chemistry Education
Course: Integrated undergraduate and graduate university study of Biology and Chemistry Education
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: magistar/magistra edukacije biologije i kemije (magistar/magistra edukacije biologije i kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-04-20 10:26:21