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master's thesis
Procjena toksičnosti vode rijeke Save pomoću modelnih organizama
Zagreb: University of Zagreb, Faculty of Science, 2018. urn:nbn:hr:217:082262
Cota, Anja Sima
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Cota, A. S. (2018). Procjena toksičnosti vode rijeke Save pomoću modelnih organizama (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:082262

Cota, Anja Sima. "Procjena toksičnosti vode rijeke Save pomoću modelnih organizama." Master's thesis, University of Zagreb, Faculty of Science, 2018. https://urn.nsk.hr/urn:nbn:hr:217:082262

Cota, Anja Sima. "Procjena toksičnosti vode rijeke Save pomoću modelnih organizama." Master's thesis, University of Zagreb, Faculty of Science, 2018. https://urn.nsk.hr/urn:nbn:hr:217:082262

Cota, A. S. (2018). 'Procjena toksičnosti vode rijeke Save pomoću modelnih organizama', Master's thesis, University of Zagreb, Faculty of Science, accessed 03 July 2024, https://urn.nsk.hr/urn:nbn:hr:217:082262

Cota AS. Procjena toksičnosti vode rijeke Save pomoću modelnih organizama [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2018 [cited 2024 July 03] Available at: https://urn.nsk.hr/urn:nbn:hr:217:082262

A. S. Cota, "Procjena toksičnosti vode rijeke Save pomoću modelnih organizama", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:217:082262

Metadata
TitleProcjena toksičnosti vode rijeke Save pomoću modelnih organizama
Title (english)Toxicity assessment of the water from Sava River using algae, plants and fish
AuthorAnja Sima Cota
MentorGoran Klobučar (mentor)
MentorSanja Babić (mentor)
Committee memberGoran Klobučar (predsjednik povjerenstva)
Committee memberSandra Radić Brkanac (član povjerenstva)
Committee memberDuje Lisičić (član povjerenstva)
Committee memberJasna Lajtner (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2018-12-21, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
With the rapid expansion of modern society in both industrial and technological aspect, it is becoming important to assess the risk of compounds which are released daily in our environment in enormous quantities. For this reason, a broad spectrum of ecotoxicological assays with various model organisms has been developed. In this study, toxicity of the Sava River has been assessed by using different model organisms: freshwater algae Chlorella vulgaris, clover Trifolium repens, common wheat Triticum aestivum and zebrafish Danio rerio embryos. Model organisms have been exposed to filtered samples of the Sava River from five different locations (Jesenice, Jankomir, Hrušćica, Rugvica, and Lukavec). Pollution profiles of samples were assessed by chemical analysis. Exposure of Chlorella vulgaris to samples caused growth inhibition and decreased chlorophyll a concentration. Root and/or sprout elongation of Trifolium repens and Triticum aestivum was observed to have lowest rates after exposure to same samples in both model organisms. Exposing the zebrafish embryos to samples resulted in minor mortality rate and sublethal effects. Activity of ABC transporters was inhibited in all samples. The use of these ecotoxicological bioassays, combined with chemical analysis, has resulted in comprehensive toxicity assessment of water from five different sites in Sava River, offering basis for monitoring of lentic and lotic waters.
Abstract (english)
Uzevši u obzir brzo širenje modernog društva u industrijskom i tehnološkom aspektu, postaje nužno procijeniti rizik tvari koje se svakodnevno ispuštaju u naš okoliš u velikim količinama. U tu svrhu, razvijen je široki spektar ekotoksikoloških testova na različitim modelnim organizmima. Unutar ovog rada, istraživana je toksičnost rijeke Save upotrebom različitih modelnih organizama: slatkovodne alge Chlorella vulgaris, djeteline Trifolium repens, pšenice Triticum aestivum i embrija ribe zebrice Danio rerio. Modelni organizmi izlagani su filtriranim uzorcima rijeke Save s pet lokacija (Jesenice, Jankomir, Hrušćica, Rugvica i Lukavec). Prisutnost toksikanata na ovim postajama utvrđena je kemijskom analizom vode. Izloženost alge Chlorella vulgaris uzorcima uzrokovala je inhibiciju njihovog rasta i smanjenu koncentraciju klorofila a. Produljenje korijenja i/ili izdanka kod vrsta Trifolium repens i Triticum aestivum imalo je najnižu stopu nakon izloženosti istim uzorcima kod oba modelna organizma. Izlaganje embrija zebrica uzorcima rezultiralo je niskom stopom mortaliteta i subletalnih efekata. Aktivnost ABC transportera kod zebrica inhibirana je prilikom izlaganja svim ispitivanim uzorcima. Ovakva upotreba ekotoksikoloških testova u kombinaciji s kemijskom analizom, omogućila je sveobuhvatnu procjenu toksičnosti uzoraka rijeke Save s pet različitih postaja, ali i pružila temelj za praćenje stanja tekućica i stajaćica.
Keywords
Keywords (croatian)
Languageenglish
URN:NBNurn:nbn:hr:217:082262
Study programmeTitle: Experimental Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2020-12-21
Terms of useLicence In copyright icon
Created on2019-03-28 11:03:30