Bacteria represent a numerous and widespread group of organisms with a important role in evolution. Despite the important role they are still insufficiently researched groups of organisms. For determining the role of bacteria in soil ecosystems, their taxonomy and biodiversity should be known. The aim of this paper is the molecular characterization of bacterial communities in soil samples collected at the Skradinski buk placed in Krka National Park using the sequencing method of the next generations and determine the possible influence of vegetation on the composition of bacterial communities in analyzed soil samples. The hypothesis is that different soil samples show a large diversity of bacterial communities and that the presence of vegetation will cause the presence of certain bacterial families in the tesedt soil. The results of this study are presented in nucleotide sequences which show that different soil samples are characterized by many different bacterial communities. Some families of bacteria appear in all samples in equal shares such as family Verrucomicrobiaceae, as most present in each sample, and Sphingomonadaceae, Bryobacteracea and Zoogloeceae in a smaller proportion. Greater representation of family Verrucomicrobiaceae was shown in soils rich in vegetation which can be interpreted as a result of reciprocal links that bacterial species and plant vegetation and the fact that these bacteria present in soils rich in vegetation such as grassland because of the interconnectedness with eucariotic organisms.