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master's thesis
Učinak antiepileptika valproata na antioksidacijski sustav u neciljnim stanicama i tkivima
Zagreb: University of Zagreb, Faculty of Science, 2018. urn:nbn:hr:217:988982
Marić, Elena
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Marić, E. (2018). Učinak antiepileptika valproata na antioksidacijski sustav u neciljnim stanicama i tkivima (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:988982

Marić, Elena. "Učinak antiepileptika valproata na antioksidacijski sustav u neciljnim stanicama i tkivima." Master's thesis, University of Zagreb, Faculty of Science, 2018. https://urn.nsk.hr/urn:nbn:hr:217:988982

Marić, Elena. "Učinak antiepileptika valproata na antioksidacijski sustav u neciljnim stanicama i tkivima." Master's thesis, University of Zagreb, Faculty of Science, 2018. https://urn.nsk.hr/urn:nbn:hr:217:988982

Marić, E. (2018). 'Učinak antiepileptika valproata na antioksidacijski sustav u neciljnim stanicama i tkivima', Master's thesis, University of Zagreb, Faculty of Science, accessed 23 December 2024, https://urn.nsk.hr/urn:nbn:hr:217:988982

Marić E. Učinak antiepileptika valproata na antioksidacijski sustav u neciljnim stanicama i tkivima [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2018 [cited 2024 December 23] Available at: https://urn.nsk.hr/urn:nbn:hr:217:988982

E. Marić, "Učinak antiepileptika valproata na antioksidacijski sustav u neciljnim stanicama i tkivima", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:217:988982

Metadata
TitleUčinak antiepileptika valproata na antioksidacijski sustav u neciljnim stanicama i tkivima
Title (english)Antiepileptic valproate effect on antioxidative defence system in non-target cells and tissues
AuthorElena Marić
MentorDomagoj Đikić (mentor)
Committee memberDomagoj Đikić (predsjednik povjerenstva)
Committee memberTomislav Ivanković (član povjerenstva)
Committee memberDavor Zanella (član povjerenstva)
Committee memberŽeljka Vidaković-Cifrek (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2018-11-27, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Cilj rada bio je istražiti kako tretman antiepileptikom valproatom utječe na razine antioksidacijskih enzima in vitro i in vivo u jetri i bubregu miševa (C57Bl6) te na staničnim modelima CHO, PK15 i HEPG2 te usporediti rezultate. Nakon tretmana valproatom in vivo i in vitro, u homogenatima bubrega i jetre miševa i u stanicama iz stanične kulture, izmjerena je lipidna peroksidacija odnosno razina malonilaldehida, koncentracija ukupnog glutationa te aktivnost antioksidacijskih enzima superoksid dismutaze i katalaze. U jetri mužjaka i ženki valproat uzrokuje smanjenu koncentraciju malonilaldehida (MDA) odnosno manju lipidnu peroksidaciju u odnosu na pripadajuće kontrolne skupine. Slično je zabilježeno i u staničnom modelu, pri čemu jetrene stanice HEPG2, reagiraju sa smanjenom koncentracijom lipidne peroksidacije nakon 24 satnog tretmana s koncentracijama od 10, 70 i 100 mM valproata u staničnoj kulturi. Stanični model bubrega u kulturi, s obzirom na trend promjene antioksidacijskih parametara uglavnom slijedi slične promjene kao i u in vivo te se zaključno može reći da je PK stanični model oksidacijskog stresa uzrokovanog valproatom dobra zamjena za in vivo mišji model. Za razliku od PK15 stanica, HEPG2 stanice nisu pokazale slične promjene antioksidativnog sustava kao i jetra tretiranih miševa.
Abstract (english)
The aim of the study was to investigate how the antiepileptic valproate treatment affects the levels of antioxidative enzymes in vitro and in vivo in the liver and kidney of the mice (C57Bl6) and on the CHO, PK15 and HEPG2 cellular models and compare the results. After valproate treatment in vivo and, lipid peroxidation, ie malonilaldehyde levels, total glutathione concentration and antioxidant enzyme activity of superoxide dismutase and catalase were measured. In the liver of males and females, valproate caused a decrease in MDA or lower lipid peroxidation relative to the associated control group. Similarly, in the cell model, and the HEPG2 dairy cells reacted with reduced lipid peroxidation concentrations after 24 hours of treatment at concentrations of 10, 70 and 100 mM valproate in cell culture. The cellular model of the kidney in culture, mainly followed the similar changes as in vivo and it can be concluded that the PK cellular model of oxidative stress caused by valproate is a good replacement for an in vivo mouse model. Unlike PK15 cells, HEPG2 cells did not show similar changes in the antioxidative system as liver-treated mice.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:988982
Study programmeTitle: Experimental Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2020-11-27
Terms of useLicence In copyright icon
Created on2019-03-28 11:29:24