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master's thesis
Raznovrsnost alela lokusa STR_MICA među bolesnicima sa šećernom bolesti tipa 1 i autoimunom bolesti štitnjače
Zagreb: University of Zagreb, Faculty of Science, 2017. urn:nbn:hr:217:420966
Bošnjak, Marina
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Bošnjak, M. (2017). Raznovrsnost alela lokusa STR_MICA među bolesnicima sa šećernom bolesti tipa 1 i autoimunom bolesti štitnjače (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:420966

Bošnjak, Marina. "Raznovrsnost alela lokusa STR_MICA među bolesnicima sa šećernom bolesti tipa 1 i autoimunom bolesti štitnjače." Master's thesis, University of Zagreb, Faculty of Science, 2017. https://urn.nsk.hr/urn:nbn:hr:217:420966

Bošnjak, Marina. "Raznovrsnost alela lokusa STR_MICA među bolesnicima sa šećernom bolesti tipa 1 i autoimunom bolesti štitnjače." Master's thesis, University of Zagreb, Faculty of Science, 2017. https://urn.nsk.hr/urn:nbn:hr:217:420966

Bošnjak, M. (2017). 'Raznovrsnost alela lokusa STR_MICA među bolesnicima sa šećernom bolesti tipa 1 i autoimunom bolesti štitnjače', Master's thesis, University of Zagreb, Faculty of Science, accessed 28 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:420966

Bošnjak M. Raznovrsnost alela lokusa STR_MICA među bolesnicima sa šećernom bolesti tipa 1 i autoimunom bolesti štitnjače [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2017 [cited 2024 November 28] Available at: https://urn.nsk.hr/urn:nbn:hr:217:420966

M. Bošnjak, "Raznovrsnost alela lokusa STR_MICA među bolesnicima sa šećernom bolesti tipa 1 i autoimunom bolesti štitnjače", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:217:420966

Metadata
TitleRaznovrsnost alela lokusa STR_MICA među bolesnicima sa šećernom bolesti tipa 1 i autoimunom bolesti štitnjače
Title (english)The diversity of STR_MICA alleles among patients with type 1 diabetes and autoimmune thyroid disease
AuthorMarina Bošnjak
MentorZorana Grubić (mentor)
MentorAna Galov (mentor)
Committee memberAna Galov (predsjednik povjerenstva)
Committee memberJasna Lajtner (član povjerenstva)
Committee memberZlatko Liber (član povjerenstva)
Committee memberDomagoj Đikić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2017-02-27, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Cilj ovog rada bio je istražiti raspodjelu alela STR_MICA među bolesnicima sa šećernom bolesti tipa 1 (DMT1), autoimunim poliglandularnim sindromom tipa 3(APS3v) i autoimunom bolesti štitnjače (AITD) te utvrditi je li učestalost pojedinih alela STR_MICA ovisna o genima HLA-DRB1 ili je rezultat neravnoteže udruživanja između alela STR_MICA i gena HLA. U istraživanje je bilo uključeno 290 nesrodnih bolesnika (DMT1-86, APS3v-63 i AITD-141) koji su uspoređeni s kontrolnim ispitanicima (N=86) odgovarajućeg spola i dobi. Svim ispitanicima uzeto je 5 ml periferne krvi s EDTA iz koje je izolirana DNA. Uzorci su testirani za alele STR_MICA lančanom reakcijom polimeraze (PCR-STR) i potom analizirani elektroforezom na 6% poliakrilamidnom gelu u aparatu za automatsko određivanje dužine fragmenta DNA. Svim ispitanicama prethodno su određeni geni lokusa HLA-DRB1 metodm PCR-SSO (Sequence Specific Oligos). Najčešće zastupljeni aleli STR_MICA među istraživanim skupinama bolesnika bili su: DMT1-STR_MICA-A6 (33,7%), APS3v-STR_MICA-A5.1 (31,8%) i AITD-STR_MICA-A5.1 i STR_MICA-A6 (26,2%). Značajnu razliku u učestalosti alela STR_MICA između bolesnika i kontrole pokazao je samo alel STR_MICA-A5 među AITD bolesnicima (12,0 vs. 6,4%; p=0,05). Geni HLA-DRB1*04 i HLA-DRB1*03 su statistički značajno češće zastupljeni među bolesnicima s DMT1 i APS3V-om, a samo gen HLA-DRB1*04 među bolesnicima AITD-om. Raspodjela alela STR_MICA u istraživanim bolestima ovisna je o genima HLA-DRB1.
Abstract (english)
The aim of the present study was to investigate the polymorphism of STR_MICA alleles among patients with type 1 diabetes (DMT1), autoimmune polyglandular syndrome type 3 (APS3v) and autoimmune thyroid diseases (AITD), and to determine whether the association between STR_MICA alleles and these diseases is independent or result of linkage disequilibrium with HLA-DRB1 genes. Two hundred and ninety unrelated patients were included (DMT1-86, APS3v-63, and AITD-141). Eighty-six unrelated healthy matched individuals served as control. All samples were tested by polymerase chain reaction (PCR) and ran on 6% polyacrylamide gel in an automated machine for determination of length of alleles, while DRB1 genes were typed by PCR-SSO (Sequence Specific Oligos) method. The most frequent STR_MICA alleles were as follows: DMT1-STR_MICA-A6 (33.7%), APS3v-STR_MICA-A5.1 (31.8%), and AITD-STR_MICA-A5.1 and STR_MICA-A6 (26.2%, each). Only STR_MICA-A5 allele showed significant difference (p=0.05) between AITD patients and controls (12.0 vs. 6.4%). HLA-DRB1*04 and HLA-DRB1*03 genes were significantly more present among DMT1 patients and APS3V patients, while among AITD patients only HLA-DRB1*04 gene reached significant p value. The distribution of STR_MICA alleles in investigated diseases is result of linkage disequilibrium with HLA-DRB1 genes.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:420966
Study programmeTitle: Experimental Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-04-20 10:47:35