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master's thesis
Glikozilacija imunoglobulina G u osoba s Downovim sindromom
Zagreb: University of Zagreb, Faculty of Science, 2019. urn:nbn:hr:217:978119
Cindrić, Ana
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Cindrić, A. (2019). Glikozilacija imunoglobulina G u osoba s Downovim sindromom (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:978119

Cindrić, Ana. "Glikozilacija imunoglobulina G u osoba s Downovim sindromom." Master's thesis, University of Zagreb, Faculty of Science, 2019. https://urn.nsk.hr/urn:nbn:hr:217:978119

Cindrić, Ana. "Glikozilacija imunoglobulina G u osoba s Downovim sindromom." Master's thesis, University of Zagreb, Faculty of Science, 2019. https://urn.nsk.hr/urn:nbn:hr:217:978119

Cindrić, A. (2019). 'Glikozilacija imunoglobulina G u osoba s Downovim sindromom', Master's thesis, University of Zagreb, Faculty of Science, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:978119

Cindrić A. Glikozilacija imunoglobulina G u osoba s Downovim sindromom [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2019 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:217:978119

A. Cindrić, "Glikozilacija imunoglobulina G u osoba s Downovim sindromom", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:217:978119

Metadata
TitleGlikozilacija imunoglobulina G u osoba s Downovim sindromom
Title (english)Immunoglobulin G glycosylation in people with Down syndrome
AuthorAna Cindrić
MentorGordan Lauc (mentor)
MentorDamjan Franjević (mentor)
Committee memberDamjan Franjević (predsjednik povjerenstva)
Committee memberSven Jelaska (član povjerenstva)
Committee memberBiljana Balen (član povjerenstva)
Committee memberInga Marijanović (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2019-02-19, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Glikozilacija je posttranslacijska modifikacija koja utječe na strukturu i funkciju proteina. Promjene u glikozilaciji imunoglobulina G (IgG), važne efektorske molekule imunološkog sustava, usko koreliraju s kronološkom dobi, a uočene su i kod brojnih bolesti. Jedan od simptoma Downovog sindroma je preuranjeno starenje, koje se posebno očituje u promjenama središnjeg živčanog sustava i imunološkog sustava. Cilj istraživanja bio je utvrditi imaju li osobe s Downovim sindromom različit profil glikozilacije IgG-a u odnosu na njihove zdrave vršnjake te, ukoliko imaju, u smislu IgG-a kao biomarkera starenja, ustanoviti očitava li se preuranjeno starenje karakteristično za osobe s Downovim sindromom u njihovom glikolizacijskom profilu IgG-a. Također, zbog prisutnosti treće kopije gena B3GALT5 u osoba s Downovom sindromom, istraženo je imaju li glikani IgG-a osoba s Downovim sindromom u svom sastavu galaktozu vezanu β-1,3 vezom. Analizom tekućinskom kromatografijom ultravisoke djelotvornosti otkriveno je da u osoba s Downovim sindromom postoje značajne razlike u udjelu pojedinih glikana IgG-a u odnosu na zdrave kontrole, te je ustanovljeno da te razlike odražavaju postojanje preuranjenog starenja u osoba s Downovim sindromom. Nadalje, pomoću digestija glikozidazama u osoba s Downovim sindromom unatoč prisutnosti treće kopije gena B3GALT5 nisu opažene mjerljive količine glikana IgG-a u čijem je sastavu galaktoza vezana β-1,3 vezom.
Abstract (english)
Glycosylation is a posttranslational modification that impacts the structure and function of proteins. Changes in immunoglobulin G (IgG) glycosylation correlate to age and the onset of many diseases. An important symptom of Down syndrome (DS) are premature signs of aging, which have notable consequences for the central nervous system and the immune system. The goal of this research was to determine whether people with DS show a different IgG glycan composition to their healthy peers and, if so, using IgG glycans as biomarkers of age, to see if these changes are connected to the symptom of premature aging. Also, due to the presence of a third copy of the B3GALT5 gene in DS, the existence of β-1,3 linked galactose in DS IgG glycans was researched. Ultra performance liquid chromatography analysis of IgG glycans of people with DS and their healthy peers clarified that there are significant differences in the composition of their IgG glycans, and also that these differences in composition correlate to premature aging. Furthermore, despite the presence of the third copy of the B3GALT5 gene, the use of glycosidase digestions did not detect measurable quantities of glycans containing β-1,3 linked galactose.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:978119
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2021-02-19
Terms of useLicence In copyright icon
Created on2019-04-02 08:23:28