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master's thesis
Genotipizacija izolata virusa hepatitisa A iz epidemije u Hrvatskoj 2017.
Zagreb: University of Zagreb, Faculty of Science, 2019. urn:nbn:hr:217:940941
Ujević, Josip
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Ujević, J. (2019). Genotipizacija izolata virusa hepatitisa A iz epidemije u Hrvatskoj 2017. (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:940941

Ujević, Josip. "Genotipizacija izolata virusa hepatitisa A iz epidemije u Hrvatskoj 2017.." Master's thesis, University of Zagreb, Faculty of Science, 2019. https://urn.nsk.hr/urn:nbn:hr:217:940941

Ujević, Josip. "Genotipizacija izolata virusa hepatitisa A iz epidemije u Hrvatskoj 2017.." Master's thesis, University of Zagreb, Faculty of Science, 2019. https://urn.nsk.hr/urn:nbn:hr:217:940941

Ujević, J. (2019). 'Genotipizacija izolata virusa hepatitisa A iz epidemije u Hrvatskoj 2017.', Master's thesis, University of Zagreb, Faculty of Science, accessed 29 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:940941

Ujević J. Genotipizacija izolata virusa hepatitisa A iz epidemije u Hrvatskoj 2017. [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2019 [cited 2024 November 29] Available at: https://urn.nsk.hr/urn:nbn:hr:217:940941

J. Ujević, "Genotipizacija izolata virusa hepatitisa A iz epidemije u Hrvatskoj 2017.", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:217:940941

Metadata
TitleGenotipizacija izolata virusa hepatitisa A iz epidemije u Hrvatskoj 2017.
Title (english)Molecular typing of hepatitis A virus isolates from epidemic in 2017. in Croatia
AuthorJosip Ujević
MentorIvana Grgić (mentor)
MentorSilvija Černi (mentor)
Committee memberSilvija Černi (predsjednik povjerenstva)
Committee memberZlatko Liber (član povjerenstva)
Committee memberMaja Matulić (član povjerenstva)
Committee memberIvana Maguire (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2019-02-18, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Virus hepatitisa A (HAV) uzročnik je hepatitisa A, akutnog oboljenja jetre. Virus se prenosi fekalno-oralnim putem, direktnim kontaktom sa zaraženom osobom ili konzumacijom zaražene hrane. Analizom genomske regije VP1-2A razlikuje se šest HAV-genotipova. Genotipovi koji inficiraju čovjeka su I, II i III s podtipovima A i B. Različiti genotipovi pokazuju različitu geografsku distribuciju. Prema podacima Europskog centra za sprječavanje i kontrolu bolesti u 2017. broj oboljelih od hepatitisa A u Europi neočekivano je porastao. U Hrvatskoj je krajem iste godine zabilježen porast broja zaraženih, prvo unutar populacije muškaraca koji imaju spolne odnose s muškarcima (MSM), a zatim i u općoj populaciji. Sekvenciranjem dijela VP1/P2A regije Sangerovom metodom te analizom sekvencija identificirano je ukupno sedam sojeva, koji pripadaju genotipu IA i IB. Iz rezultata istraživanja može se zaključiti da je u Hrvatskoj bilo ukupno sedam nezavisnih izvora zaraze. Dva soja virusa UK/Spain i Europride, koja su povezana s europskom epidemijom, dominiraju u zaraženih osoba u Hrvatskoj te su pretežito prisutna u MSM-populaciji. U općoj populaciji je identificirano pet sojeva virusa, za koji način prijenosa nije utvrđen. Dobiveni podaci o genotipovima i podtipovima analiziranih izolata mogu poslužiti za molekularno epidemiološku studiju rekonstrukcije puta rasprostranjivanja virusa hepatitis A unutar i između zaraženih populacija.
Abstract (english)
Hepatitis A virus (HAV) causes hepatitis A, an acute liver disease. It is transmitted by fecal-oral route, by direct contact with infected person or by contaminated food consumption. Six genotypes are distinguished by analyzing the genomic region VP1-2A. Genotypes, I, II and III, divided into subtypes A and B, infect humans. Different genotypes show different geographical distribution. According to the European Center for Disease Prevention and Control data, the number of hepatitis A cases in Europe unexpectedly increased in 2017. By the end of the same year, there was an increase in the number of infected in Croatia, first noticed in population of men who have sex with the men (MSM) and then in general population. Seven virus outbreak strains were detected by Sanger sequencing of VP1/P2A region followed by sequence analysis. Results suggest seven independent sources of infection in Croatia. Two virus strains, UK/Spain and EuroPride, linked to epidemic in Europe, dominate in Croatia and are predominantly present in MSM population. In general population, five strains of virus have been detected, for which method of transmission has not been elucidated. The observed results can be used for molecular epidemiological reconstruction of the virus transmission between and within infected population.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:940941
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2019-04-02 08:55:38