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master's thesis
Utjecaj transkripcijskih regulatora bakterije Escherichia coli na učestalost formiranja čistina nakon infekcije bakteriofagom T4
Zagreb: University of Zagreb, Faculty of Science, 2019. urn:nbn:hr:217:607257
Srakočić, Sanja
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Srakočić, S. (2019). Utjecaj transkripcijskih regulatora bakterije Escherichia coli na učestalost formiranja čistina nakon infekcije bakteriofagom T4 (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:607257

Srakočić, Sanja. "Utjecaj transkripcijskih regulatora bakterije Escherichia coli na učestalost formiranja čistina nakon infekcije bakteriofagom T4." Master's thesis, University of Zagreb, Faculty of Science, 2019. https://urn.nsk.hr/urn:nbn:hr:217:607257

Srakočić, Sanja. "Utjecaj transkripcijskih regulatora bakterije Escherichia coli na učestalost formiranja čistina nakon infekcije bakteriofagom T4." Master's thesis, University of Zagreb, Faculty of Science, 2019. https://urn.nsk.hr/urn:nbn:hr:217:607257

Srakočić, S. (2019). 'Utjecaj transkripcijskih regulatora bakterije Escherichia coli na učestalost formiranja čistina nakon infekcije bakteriofagom T4', Master's thesis, University of Zagreb, Faculty of Science, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:607257

Srakočić S. Utjecaj transkripcijskih regulatora bakterije Escherichia coli na učestalost formiranja čistina nakon infekcije bakteriofagom T4 [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2019 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:217:607257

S. Srakočić, "Utjecaj transkripcijskih regulatora bakterije Escherichia coli na učestalost formiranja čistina nakon infekcije bakteriofagom T4", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:217:607257

Metadata
TitleUtjecaj transkripcijskih regulatora bakterije Escherichia coli na učestalost formiranja čistina nakon infekcije bakteriofagom T4
Title (english)The effect of Escherichia coli transcriptional regulators on efficiency of plaque formation of T4 bacteriophage
AuthorSanja Srakočić
MentorIvana Ivančić Baće (mentor)
Committee memberIvana Ivančić Baće (predsjednik povjerenstva)
Committee memberMartina Šeruga Musić (član povjerenstva)
Committee memberDuje Lisičić (član povjerenstva)
Committee memberNataša Bauer (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2019-02-15, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Proteini H-NS i StpA su globalni transkripcijski represori iz bakterije Escherichia coli koji samostalno i zajedno reguliraju ekspresiju oko 20% gena. Njihova ekspresija je različito regulirana, gdje jedan drugog negativno reguliraju, a ekspresija im ovisi i o temperaturi inkubiranja. Geni čija je ekspresija regulirana samo proteinom H-NS se zovu unilateralni geni, a geni čija je ekspresija regulirana proteinima H-NS i StpA se zovu epistatski geni. Iz dosadašnjih istraživanja uočeno je da bakteriofag T4 iz nepoznatih razloga ne može formirati čistine u inficiranim bakterijskim stanicama Δhns pri temperaturi inkubacije od 30 oC. Cilj ovog rada bio je istražiti ulogu proteina StpA, nekoliko epistatskih gena, te proteaze Lon na uspješnost formiranja čistina bakteriofaga T4 u stanicama Δhns. Dobiveni rezultati ukazuju da nastanak čistina faga T4 u stanicama hns ovisi o proteinu StpA. Nedostatak proteaze Lon nije uspostavio nastanak čistina, kao niti jedan od istraživanih epistatskih gena cbl, cspA, dnaK, groL, htpG, rnc i sodA. Čistine faga T4 su uočene u soju E. coli B Δhns koji je prirodni mutant lon, što ukazuje da formiranje čistina faga T4 ovisi o nepoznatom razlikovnom genu između E. coli K-12 i E. coli B.
Abstract (english)
H-NS and StpA proteins are global transcriptional repressors of Escherichia coli that independently and together regulate the expression of about 20% of the genes. Their expression is regulated differently, they negatively regulate one another, and their expression also depends on the temperature of incubation. The genes whose expression is regulated by H-NS alone are called unilateral genes, and the genes whose expression is regulated by H-NS and StpA proteins are called epistatic genes. From recent studies, it has been found that bacteriophage T4 can not form plaques in infected Δhns cells incubated at a 30 °C for an unknown reasons. The aim of this study was to investigate the role of StpA protein, several epistatic genes and Lon protease on the efficiency of T4 bacteriophage plaque formation in Δhns cells. The obtained results indicate that T4 bacteriophage plaque formation depends on the StpA protein. The lack of protease Lon did not revert the plaque formation, as none of the investigated epistatic genes cbl, cspA, dnaK, groL, htpG, rnc and sodA. T4 bacteriophage plaques were observed in the E. coli B Δhns cells that is a natural lon mutant, indicating that plaque formation depends on the unidentified distinct gene between E. coli K-12 and E. coli B.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:607257
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2020-02-15
Terms of useLicence In copyright icon
Created on2019-04-02 09:02:25