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master's thesis
Karakterizacija novonastalog tkiva nakon biopsije hrskavice nosne pregrade
Zagreb: University of Zagreb, Faculty of Science, 2019. urn:nbn:hr:217:798467
Brezak, Matea
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Brezak, M. (2019). Karakterizacija novonastalog tkiva nakon biopsije hrskavice nosne pregrade (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:798467

Brezak, Matea. "Karakterizacija novonastalog tkiva nakon biopsije hrskavice nosne pregrade." Master's thesis, University of Zagreb, Faculty of Science, 2019. https://urn.nsk.hr/urn:nbn:hr:217:798467

Brezak, Matea. "Karakterizacija novonastalog tkiva nakon biopsije hrskavice nosne pregrade." Master's thesis, University of Zagreb, Faculty of Science, 2019. https://urn.nsk.hr/urn:nbn:hr:217:798467

Brezak, M. (2019). 'Karakterizacija novonastalog tkiva nakon biopsije hrskavice nosne pregrade', Master's thesis, University of Zagreb, Faculty of Science, accessed 23 December 2024, https://urn.nsk.hr/urn:nbn:hr:217:798467

Brezak M. Karakterizacija novonastalog tkiva nakon biopsije hrskavice nosne pregrade [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2019 [cited 2024 December 23] Available at: https://urn.nsk.hr/urn:nbn:hr:217:798467

M. Brezak, "Karakterizacija novonastalog tkiva nakon biopsije hrskavice nosne pregrade", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:217:798467

Metadata
TitleKarakterizacija novonastalog tkiva nakon biopsije hrskavice nosne pregrade
Title (english)Characterization of newly-formed tissue after nasal septum cartilage biopsy
AuthorMatea Brezak
MentorInga Marijanović (mentor)
Committee memberInga Marijanović (predsjednik povjerenstva)
Committee memberNada Oršolić (član povjerenstva)
Committee memberRomana Gračan (član povjerenstva)
Committee memberTomislav Ivanković (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2019-02-12, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Danas se u liječenju oštećenja zglobne hrskavice ili hrskavice nosne pregrade sve češće koriste tkivni presatci dobiveni metodama tkivnog inženjerstva. Prilikom uzgoja tkivnih presadaka potreban je izvor stanica koje će se nasaditi na nosač, te se u tu svrhu uzima biopsija hrskavice nosne pregrade. Uslijed tog postupka, na mjestu biopsije ostaje praznina koja se tijekom vremena ispuni tkivom nepoznatog porijekla i morfologije. Do sada nisu provedena istraživanja na velikim modelnim organizmima koja bi dokazala posjeduje li novonastalo tkivo svojstva potrebna svojstva da bi se održala normalna funkcija pregrade. Ukoliko se formira novo hrskavično tkivo funkcionalnost ne bi bila ugrožena te bi se omogućilo ponovno uzimanje hondrocita s istoga mjesta. Cilj ovog istraživanja je putem histoloških i imunohistokemijskih bojanja, kao i analizom ekspresije gena metodom qPCR okarakterizirati novonastalo tkivo na mjestu biopsije. Histološka analiza je pokazalaniski regenerativni potencijal hrskavičnog tkiva kao i ulogu perihondrija u stvaranju novog tkiva. Analiza ekspresije gena novonastalog tkiva je potvrdila histološke rezultate.. Dodatno, visoka ekspresija gena COL 1, COL 4 i CRABP 1 potvrdila je značajnu ulogu vanjskog fibroznog sloja perihondrija u nastanku novog tkiva
Abstract (english)
Tissue engineering has become one of the most studied fields with clinical application. When producing cartilage tissue in vitro, nasal septum biopsy is taken in order to harvest chondrocytes that will be seeded on scaffolds. Following this procedure, the nasal septum is left with a perforation that fills in over time. The newly-formed tissue may not possess functional properties characteristic for septum cartilage, whereas if the neocartilage forms it would enable chondrocyte reharvesting and confirm normal septum function. To this time, no one has addressed the issue of tissue formation in the place of the perforation on a large animal model. Therefore, in this study, newly-formed tissue was characterized using histological and immunohistochemical stainings including analysis of cartilage and perichondrium marker gene expression. Low regenerative potential of cartilage was confirmed as no formation of similar tissue was observed over time. Perichondral origin of newly-formed tissue was confirmed both histologically and through gene expression analysis. Additionally, high expression of COL 1, COL 4 and CRABP 1 genes indicate a significant role of outer perichondrium layer in tissue genesis.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:798467
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2020-02-12
Terms of useLicence In copyright icon
Created on2019-04-02 10:20:29