prikaz prve stranice dokumenta The role of bridging fibers in the architecture of the metaphase mitotic spindle
  Download
PDF 21.85 MB
doctoral thesis
The role of bridging fibers in the architecture of the metaphase mitotic spindle
Zagreb: University of Zagreb, Faculty of Science, 2019. urn:nbn:hr:217:642253
Polak, Bruno
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Polak, B. (2019). The role of bridging fibers in the architecture of the metaphase mitotic spindle (Doctoral thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:642253

Polak, Bruno. "The role of bridging fibers in the architecture of the metaphase mitotic spindle." Doctoral thesis, University of Zagreb, Faculty of Science, 2019. https://urn.nsk.hr/urn:nbn:hr:217:642253

Polak, Bruno. "The role of bridging fibers in the architecture of the metaphase mitotic spindle." Doctoral thesis, University of Zagreb, Faculty of Science, 2019. https://urn.nsk.hr/urn:nbn:hr:217:642253

Polak, B. (2019). 'The role of bridging fibers in the architecture of the metaphase mitotic spindle', Doctoral thesis, University of Zagreb, Faculty of Science, accessed 20 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:642253

Polak B. The role of bridging fibers in the architecture of the metaphase mitotic spindle [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Science; 2019 [cited 2024 November 20] Available at: https://urn.nsk.hr/urn:nbn:hr:217:642253

B. Polak, "The role of bridging fibers in the architecture of the metaphase mitotic spindle", Doctoral thesis, University of Zagreb, Faculty of Science, Zagreb, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:217:642253

Metadata
TitleThe role of bridging fibers in the architecture of the metaphase mitotic spindle
Title (croatian)Uloga premošćujućih vlakana u arhitekturi metafaznoga diobenoga vretena
AuthorBruno Polak
MentorInga Marijanović (mentor)
Committee memberJuraj Simunić (predsjednik povjerenstva)
Committee memberMarin Barišić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2019, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Universal decimal classification
(UDC)		577 - Biochemistry. Molecular biology. Biophysics
Abstract
Mitosis is a fundamental process during which the genetic material is transferred to daughter cells and is orchestrated by the mitotic spindle. Between two opposite spindle poles microtubules form bundles that interact with chromosomes. Bundles that bind to kinetochores on the chromosome are called kinetochore fibers, whilst certain microtubules form antiparallel overlap bundles in the central part of the spindle. With this research localization and distribution of antiparallel overlap bundles in metaphase and the spatial organization of these bundles and kinetochore fibers possess will be investigated. By using confocal microscope whole metaphase spindles will be imaged which will enable to determine the number of overlap regions and their association with kinetochore fibers. Further work will be focused on understanding the spatial organization of the whole mitotic spindle. Used approach revealed that nearly all antiparallel fibers are linked with kinetochore fibers and that human mitotic spindle is a chiral structure.
Abstract (croatian)
Mitoza je fundamentalan process kojim se genetička informacija prenosi na stanice kćeri, a struktura koja upravlja ovim procesom je diobeno vreteno, Između dva nasuprotna pola vretena pružaju se vlakna mikrotubula koja stvaraju interakcije sa kromosomima. Vlakna koja se vežu za kinetohore na kromosomima nazivaju se kinetohorna vlakna. Neki od ostalih svežnjeva stvaraju antiparalelne preklapajuće regije u centralnom djelu diobenog vretena. U ovom radu ispitati će se lokalizacija i zastupljenost antiparalelnih preklapajućih regija u metafazi te će se istražiti prostorna organizacija navedenih i kinethornih vlakana. Uz pomoć metode konfokalne mikroskopije snimati će se cijela vretena što će omogućiti utvrđivanje broja antiparalelnih svežnjeva, a odgovoriti će na pitanje njihove povezanosti sa kinetohornim vlaknima. Daljnji rad biti će usmjeren na razumijevanje prostorne organizacije metafaznog diobenog vretena. Uz primjenu navedenog pristupa, otkriveno je da su antiparalelna vlakna povezana sa kinetohornim vlaknima te da je diobeno vreteno u ljudskim stanicama kiralna struktura.
Keywords
Keywords (croatian)
Languageenglish
URN:NBNurn:nbn:hr:217:642253
Study programmeTitle: Biology
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija (doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2019-07-16 09:40:27