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master's thesis
Utjecaj sirtuina 3 u tretmanu hiperoksijom na tumorske karakteristike trostruko negativnih stanica raka dojke (MDA-MB-231)
Zagreb: University of Zagreb, Faculty of Science, 2019. urn:nbn:hr:217:742016
Marčinko, Dora
University of Zagreb
Faculty of Science
Department of Chemistry

Institutional repository: Repository of the Faculty of Science

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Marčinko, D. (2019). Utjecaj sirtuina 3 u tretmanu hiperoksijom na tumorske karakteristike trostruko negativnih stanica raka dojke (MDA-MB-231) (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:742016

Marčinko, Dora. "Utjecaj sirtuina 3 u tretmanu hiperoksijom na tumorske karakteristike trostruko negativnih stanica raka dojke (MDA-MB-231)." Master's thesis, University of Zagreb, Faculty of Science, 2019. https://urn.nsk.hr/urn:nbn:hr:217:742016

Marčinko, Dora. "Utjecaj sirtuina 3 u tretmanu hiperoksijom na tumorske karakteristike trostruko negativnih stanica raka dojke (MDA-MB-231)." Master's thesis, University of Zagreb, Faculty of Science, 2019. https://urn.nsk.hr/urn:nbn:hr:217:742016

Marčinko, D. (2019). 'Utjecaj sirtuina 3 u tretmanu hiperoksijom na tumorske karakteristike trostruko negativnih stanica raka dojke (MDA-MB-231)', Master's thesis, University of Zagreb, Faculty of Science, accessed 20 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:742016

Marčinko D. Utjecaj sirtuina 3 u tretmanu hiperoksijom na tumorske karakteristike trostruko negativnih stanica raka dojke (MDA-MB-231) [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2019 [cited 2024 November 20] Available at: https://urn.nsk.hr/urn:nbn:hr:217:742016

D. Marčinko, "Utjecaj sirtuina 3 u tretmanu hiperoksijom na tumorske karakteristike trostruko negativnih stanica raka dojke (MDA-MB-231)", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:217:742016

Metadata
TitleUtjecaj sirtuina 3 u tretmanu hiperoksijom na tumorske karakteristike trostruko negativnih stanica raka dojke (MDA-MB-231)
Title (english)The role of Sirtuin 3 upon hyperoxic treatment on tumorigenic characteristics of tripple-negative breast cancer cells (MDA-MB-231)
AuthorDora Marčinko
MentorSandra Sobočanec (mentor)
Committee memberMorana Dulić (predsjednik povjerenstva)
Committee memberIvica Đilović (član povjerenstva)
Committee memberIva Juranović Cindrić (član povjerenstva)
Committee memberAleksandra Maršavelski (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Chemistry)
Zagreb
Defense date and country2019-07-05, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
Sirtuin 3 (Sirt3) je glavna mitohondrijska NAD+-ovisna deacetilaza s ulogom u održavanju energetske homeostaze. U nedostatku Sirt3 dolazi do povišenog oksidacijskog stresa, visokih razina reaktivnih kisikovih vrsta, oštećenja DNA i genomske nestabilnosti te kontinuiranog rasta tumorskih stanica. Sirt3, ovisno o tipu stanica, pokazuje dvojnu ulogu u stanicama raka. Ovo istraživanje je provedeno na trostruko negativnim stanicama raka dojke MDA-MB-231 s nadeksprimiranim Sirt3 u uvjetima normoksije i hiperoksije (95% O2) kako bi se ispitao utjecaj Sirt3 na proliferaciju, metaboličku aktivnost i mitohondrijsku funkciju. Sirt3 je poboljšao funkciju mitohondrija (pojačani metabolizam, mitohondrijska masa i potencijal), ali unatoč aktivnijim antioksidativnim enzimima, uzrokovao je i više mitohondrijskih ROS-ova, veće oštećenje DNA te manju sposobnost stvaranja kolonija, s izraženijim efektom u hiperoksiji. Stoga su Sirt3 i hiperoksija pokazali i tumor-supresorski učinak u MDA-MB-231 stanicama, te bi se trebali proučiti dodatni mehanizmi njihovog utjecaja u svrhu pronalaska potencijalnih terapeutskih meta u liječenju trostruko negativnih tumora dojke.
Abstract (english)
Sirtuin 3 (Sirt3) is the major mitochondrial NAD+-dependent deacetylase with role in maintaining the energetic homeostasis. The absence of Sirt3 causes elevated oxidative stress, high levels of reactive oxygen species (ROS), DNA damage and genomic instability, as well as a continuous tumor cell growth. Sirt3 has a dual role in cancer cells depending on the cell type. This study has been conducted on the triple-negative breast cancer cells MDA-MB-231 with overexpressed Sirt3 in normoxia and hyperoxia (95% O2) to investigate the effect of Sirt3 on proliferation, metabolic activity and mitochondrial function. Sirt3 improved the function of mitochondria (increased metabolism, mitochondrial mass and potential), however, despite more active antioxidant enzymes, it also induced more mitochondrial ROS, greater DNA damage and lower colony forming, with a more pronounced effect in hyperoxia. Therefore Sirt3 and hyperoxia have a tumor-suppressing effect on MDA-MB-231 and the additional mechanism of their effect should be further explored in order to find potential therapeutic targets in the treatment of triple-negative breast cancer.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:742016
Study programmeTitle: Graduate university studies in chemistry; specializations in: Research programme
Course: Research programme
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2021-07-05
Terms of useLicence In copyright icon
Created on2019-07-16 13:40:50