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master's thesis
Replikacija DNA u tkivu zahvaćenom folikularnim limfomom
Zagreb: University of Zagreb, Faculty of Science, 2015. urn:nbn:hr:217:338604
Bašić Palković, Pamela
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Bašić Palković, P. (2015). Replikacija DNA u tkivu zahvaćenom folikularnim limfomom (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:338604

Bašić Palković, Pamela. "Replikacija DNA u tkivu zahvaćenom folikularnim limfomom." Master's thesis, University of Zagreb, Faculty of Science, 2015. https://urn.nsk.hr/urn:nbn:hr:217:338604

Bašić Palković, Pamela. "Replikacija DNA u tkivu zahvaćenom folikularnim limfomom." Master's thesis, University of Zagreb, Faculty of Science, 2015. https://urn.nsk.hr/urn:nbn:hr:217:338604

Bašić Palković, P. (2015). 'Replikacija DNA u tkivu zahvaćenom folikularnim limfomom', Master's thesis, University of Zagreb, Faculty of Science, accessed 25 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:338604

Bašić Palković P. Replikacija DNA u tkivu zahvaćenom folikularnim limfomom [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2015 [cited 2024 November 25] Available at: https://urn.nsk.hr/urn:nbn:hr:217:338604

P. Bašić Palković, "Replikacija DNA u tkivu zahvaćenom folikularnim limfomom", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2015. Available at: https://urn.nsk.hr/urn:nbn:hr:217:338604

Metadata
TitleReplikacija DNA u tkivu zahvaćenom folikularnim limfomom
Title (english)DNA replication in follicular lymphoma tissue
AuthorPamela Bašić Palković
MentorPetra Korać (mentor)
Committee memberPetra Korać (predsjednik povjerenstva)
Committee memberZoran Tadić (član povjerenstva)
Committee memberTatjana Bakran-Petricioli (član povjerenstva)
Committee memberMaja Matulić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2015-09-29, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Folikularni limfom predstavlja B–staničnu neoplazmu građenu od stanica porijeklom iz folikularnih centara (germinativnih centara) limfnog čvora koje eksprimiraju BCL6, CD10 i najčešće BCL2. Translokacija t(14;18)(q32;q21), koja dovodi do pojačane ekspresije antiapoptotskog proteina BCL2, predstavlja karakterističnu genetičku promjenu folikularnog limfoma. Folikularni limfom jedini je limfom koji ima graduse - stupnjeve progresije. Jedan od potencijalnih putova razvoja viših stupnjeva progresije (postoje 3 stupnja) narušeni je proces replikacije i ponovne uspostave kromatina nakon replikacije. Cilj ovog istraživanja bio je analizirati količinu transkripta gena ključnih za proces replikacije i ponovnu uspostavu kromatina u tumorskom i netumorskom dijelu tkiva zahvaćenog folikularnim limfomom. Korišteno je 10 uzoraka limfnih čvorova zahvaćenih folikularnim limfomom gradusa 2 i 3. Tumorski i netumorski dijelovi tkiva zahvaćenog folikularnim limfomom izdvojeni su laserskom mikrodisekcijom, izolirana je RNA i reverznom transkripcijom pripremljena za analizu transkripta gena DNMT1, PCNA, MCM2, MCM7, CDT1, EZH2, GMNN i p300 lančanom reakcijom polimerazom u stvarnom vremenu. Dobiveni rezultati nisu pokazali značajne razlike u ekspresiji analiziranih gena između skupina tumorskog i netumorskog tkiva zahvaćenog folikularnim limfomom.
Abstract (english)
Follicular lymphoma is a B–cell neoplasm composed of cells originating from follicular centres (germinal centres) of lymph node that express BCL6, CD10 and, usually, BCL2. The translocation t(14;18)(q32;q21), which leads to enhanced expression of antiapoptotic BCL2 protein, is the characteristic type of genetic alteration in follicular lymphoma. Follicular lymphoma is the only lymphoma that has grades - stages of progression. One of the potential developmental pathways of higher stages of progression ( there are 3 stages) might include the process of replication and the reassembly of chromatin structure after replication. Aim of this research was to analyze the transcript levels of genes which are important for the process of replication and the re–establishment of chromatin in tumor and non–tumor part of tissue affected with the neoplasm. Ten samples of lymph nodes with follicular lymphoma grade 2 and 3 were used. Tumor and non– tumor part of the tissue were selected and gathered using laser–microdissection technology, RNA was isolated and reversely transcribed into cDNA for QPCR analysis of DNMT1, PCNA, MCM2, MCM7, CDT1, EZH2, GMNN and p300 transcripts. The results showed no significant differences in the expression of the analyzed genes between groups of tumor and, surrounding, non–tumor tissue of affected lymph nodes.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:338604
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-04-21 08:59:50